Detección de los genes de las exotoxinas pirogénicas SpeA, SpeB y SpeC en cepas chilenas de Streptococcus pyogenes y su asociación con la clínica

Background: The virulence of Streptococcus pyogenes is determined by a variety of structural molecules, toxins and complex enzymes. Pyrogenic exotoxins cause fever, erythematous reactions, cytotoxic and immunological effects. Aim: To assess the frequency of speA, speB and speC genes in Chilean Strep...

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Autores principales: Ulloa F,María Teresa, Giglio M,María Soledad, Porte T,Lorena, Santa Cruz A,Adriana, McNab M,Paul, Fica C,Alberto, Pinto C,María Eugenia, Kawaguchi G,Keyko, Carmi K,Alejandra
Lenguaje:Spanish / Castilian
Publicado: Sociedad Médica de Santiago 2000
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Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872000000100004
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spelling oai:scielo:S0034-988720000001000042000-05-25Detección de los genes de las exotoxinas pirogénicas SpeA, SpeB y SpeC en cepas chilenas de Streptococcus pyogenes y su asociación con la clínicaUlloa F,María TeresaGiglio M,María SoledadPorte T,LorenaSanta Cruz A,AdrianaMcNab M,PaulFica C,AlbertoPinto C,María EugeniaKawaguchi G,KeykoCarmi K,Alejandra Exotoxins Gene frecuency Polymerase chain reaction Background: The virulence of Streptococcus pyogenes is determined by a variety of structural molecules, toxins and complex enzymes. Pyrogenic exotoxins cause fever, erythematous reactions, cytotoxic and immunological effects. Aim: To assess the frequency of speA, speB and speC genes in Chilean Streptococcus pyogenes strains and their association with the invasiveness of infections. Material and methods: The genes for pyrogenic exotoxins SpeA, SpeB and SpeC were determined by polymerase chain reactions in 114 strains of group A Streptococcus pyogenes isolated from Chilean patients with invasive or non invasive infections. Results: The gene for SpeA was present in 30.7% of isolates, the gene for SpeB was present in 69.3% and the gen for SpeC in 44.7% of isolates. The gene for SpeA was present in 20 of 33 invasive infections and in 15 of 81 non invasive infections (p <0.0001). On the contrary, the gene for SpeC was present in 11 of 33 invasive infections and in 41 of 81 non invasive infections (p <0.05). The frequency of speB was similar in invasive and non invasive infections. Conclusions: There is a clear relationship between the presence of SpeA genes and the severity of infections caused by Streptococcus pyogenes. (Rev Méd Chile 2000; 128: 27-34)info:eu-repo/semantics/openAccessSociedad Médica de SantiagoRevista médica de Chile v.128 n.1 20002000-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872000000100004es10.4067/S0034-98872000000100004
institution Scielo Chile
collection Scielo Chile
language Spanish / Castilian
topic Exotoxins
Gene frecuency
Polymerase chain reaction
spellingShingle Exotoxins
Gene frecuency
Polymerase chain reaction
Ulloa F,María Teresa
Giglio M,María Soledad
Porte T,Lorena
Santa Cruz A,Adriana
McNab M,Paul
Fica C,Alberto
Pinto C,María Eugenia
Kawaguchi G,Keyko
Carmi K,Alejandra
Detección de los genes de las exotoxinas pirogénicas SpeA, SpeB y SpeC en cepas chilenas de Streptococcus pyogenes y su asociación con la clínica
description Background: The virulence of Streptococcus pyogenes is determined by a variety of structural molecules, toxins and complex enzymes. Pyrogenic exotoxins cause fever, erythematous reactions, cytotoxic and immunological effects. Aim: To assess the frequency of speA, speB and speC genes in Chilean Streptococcus pyogenes strains and their association with the invasiveness of infections. Material and methods: The genes for pyrogenic exotoxins SpeA, SpeB and SpeC were determined by polymerase chain reactions in 114 strains of group A Streptococcus pyogenes isolated from Chilean patients with invasive or non invasive infections. Results: The gene for SpeA was present in 30.7% of isolates, the gene for SpeB was present in 69.3% and the gen for SpeC in 44.7% of isolates. The gene for SpeA was present in 20 of 33 invasive infections and in 15 of 81 non invasive infections (p <0.0001). On the contrary, the gene for SpeC was present in 11 of 33 invasive infections and in 41 of 81 non invasive infections (p <0.05). The frequency of speB was similar in invasive and non invasive infections. Conclusions: There is a clear relationship between the presence of SpeA genes and the severity of infections caused by Streptococcus pyogenes. (Rev Méd Chile 2000; 128: 27-34)
author Ulloa F,María Teresa
Giglio M,María Soledad
Porte T,Lorena
Santa Cruz A,Adriana
McNab M,Paul
Fica C,Alberto
Pinto C,María Eugenia
Kawaguchi G,Keyko
Carmi K,Alejandra
author_facet Ulloa F,María Teresa
Giglio M,María Soledad
Porte T,Lorena
Santa Cruz A,Adriana
McNab M,Paul
Fica C,Alberto
Pinto C,María Eugenia
Kawaguchi G,Keyko
Carmi K,Alejandra
author_sort Ulloa F,María Teresa
title Detección de los genes de las exotoxinas pirogénicas SpeA, SpeB y SpeC en cepas chilenas de Streptococcus pyogenes y su asociación con la clínica
title_short Detección de los genes de las exotoxinas pirogénicas SpeA, SpeB y SpeC en cepas chilenas de Streptococcus pyogenes y su asociación con la clínica
title_full Detección de los genes de las exotoxinas pirogénicas SpeA, SpeB y SpeC en cepas chilenas de Streptococcus pyogenes y su asociación con la clínica
title_fullStr Detección de los genes de las exotoxinas pirogénicas SpeA, SpeB y SpeC en cepas chilenas de Streptococcus pyogenes y su asociación con la clínica
title_full_unstemmed Detección de los genes de las exotoxinas pirogénicas SpeA, SpeB y SpeC en cepas chilenas de Streptococcus pyogenes y su asociación con la clínica
title_sort detección de los genes de las exotoxinas pirogénicas spea, speb y spec en cepas chilenas de streptococcus pyogenes y su asociación con la clínica
publisher Sociedad Médica de Santiago
publishDate 2000
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872000000100004
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