Cáncer gástrico y regulación del crecimiento tumoral.: Estudio de marcadores de proliferación celular y del complejo proteínas p53/p21WAF1/CIP1/mdm-2

Background: p53/p21WAF1/CIP1 and mdm-2 proteins play an important role in cell cycle regulation. The study of the pathogenesis of gastric cancer is important to understand how these tumors progress during their natural history. Aim: To study the relationship between the immunohistochemical expressio...

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Autores principales:	Villaseca H,Miguel, Araya O,Juan Carlos, Roa E,Iván, Roa S,Juan Carlos
Lenguaje:	Spanish / Castilian
Publicado:	Sociedad Médica de Santiago 2000
Materias:	Immunohistochemistry Gastrectomy Neoplasm proteins Stomach neoplasms
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872000000200001
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spelling	oai:scielo:S0034-988720000002000012014-05-06Cáncer gástrico y regulación del crecimiento tumoral.: Estudio de marcadores de proliferación celular y del complejo proteínas p53/p21WAF1/CIP1/mdm-2Villaseca H,MiguelAraya O,Juan CarlosRoa E,IvánRoa S,Juan Carlos Immunohistochemistry Gastrectomy Neoplasm proteins Stomach neoplasms Background: p53/p21WAF1/CIP1 and mdm-2 proteins play an important role in cell cycle regulation. The study of the pathogenesis of gastric cancer is important to understand how these tumors progress during their natural history. Aim: To study the relationship between the immunohistochemical expression of p53/p21WAF1/CIP1 and mdm-2 and cell proliferation in gastric cancer. Material and methods: Fifty one gastrectomy specimens with gastric cancer were studied using immunohistochemistry for p53/p21WAF1/CIP1 and mdm-2. Cell proliferation was determined by immunolabelling with PCNA and Ki67 antigens. Mitosis figures were counted in 10 high power microscopic fields. Results: Patients from whom gastric cancer specimens were obtained had a mean age of 59.3 years. Ki67 and mitosis counting showed the highest correlation index with proliferation indexes studied. No correlation was found between the expression of protein complex p53/p21WAF1/CIP1 and mdm-2 and morphological characteristics of gastric tumors. Mdm-2 protein overexpression was the only marker that showed an independent correlation with cell proliferation. Moreover, mdm-2 positive tumors showed the highest proliferation indexes when p53 was not immunohistochemically over-expressed, as determined by PCNA labelling index. Conclusions: In gastric cancer, a direct correlation between mdm-2 overexpression and cell proliferation was observed. Moreover, the fact that mdm-2 positive tumors showed the highest cell proliferation when p53 was not overexpressed, entitles us to hypothesize that mdm-2 overexpression could play a major role in gastric carcinogenesis.info:eu-repo/semantics/openAccessSociedad Médica de SantiagoRevista médica de Chile v.128 n.2 20002000-02-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872000000200001es10.4067/S0034-98872000000200001
institution	Scielo Chile
collection	Scielo Chile
language	Spanish / Castilian
topic	Immunohistochemistry Gastrectomy Neoplasm proteins Stomach neoplasms
spellingShingle	Immunohistochemistry Gastrectomy Neoplasm proteins Stomach neoplasms Villaseca H,Miguel Araya O,Juan Carlos Roa E,Iván Roa S,Juan Carlos Cáncer gástrico y regulación del crecimiento tumoral.: Estudio de marcadores de proliferación celular y del complejo proteínas p53/p21WAF1/CIP1/mdm-2
description	Background: p53/p21WAF1/CIP1 and mdm-2 proteins play an important role in cell cycle regulation. The study of the pathogenesis of gastric cancer is important to understand how these tumors progress during their natural history. Aim: To study the relationship between the immunohistochemical expression of p53/p21WAF1/CIP1 and mdm-2 and cell proliferation in gastric cancer. Material and methods: Fifty one gastrectomy specimens with gastric cancer were studied using immunohistochemistry for p53/p21WAF1/CIP1 and mdm-2. Cell proliferation was determined by immunolabelling with PCNA and Ki67 antigens. Mitosis figures were counted in 10 high power microscopic fields. Results: Patients from whom gastric cancer specimens were obtained had a mean age of 59.3 years. Ki67 and mitosis counting showed the highest correlation index with proliferation indexes studied. No correlation was found between the expression of protein complex p53/p21WAF1/CIP1 and mdm-2 and morphological characteristics of gastric tumors. Mdm-2 protein overexpression was the only marker that showed an independent correlation with cell proliferation. Moreover, mdm-2 positive tumors showed the highest proliferation indexes when p53 was not immunohistochemically over-expressed, as determined by PCNA labelling index. Conclusions: In gastric cancer, a direct correlation between mdm-2 overexpression and cell proliferation was observed. Moreover, the fact that mdm-2 positive tumors showed the highest cell proliferation when p53 was not overexpressed, entitles us to hypothesize that mdm-2 overexpression could play a major role in gastric carcinogenesis.
author	Villaseca H,Miguel Araya O,Juan Carlos Roa E,Iván Roa S,Juan Carlos
author_facet	Villaseca H,Miguel Araya O,Juan Carlos Roa E,Iván Roa S,Juan Carlos
author_sort	Villaseca H,Miguel
title	Cáncer gástrico y regulación del crecimiento tumoral.: Estudio de marcadores de proliferación celular y del complejo proteínas p53/p21WAF1/CIP1/mdm-2
title_short	Cáncer gástrico y regulación del crecimiento tumoral.: Estudio de marcadores de proliferación celular y del complejo proteínas p53/p21WAF1/CIP1/mdm-2
title_full	Cáncer gástrico y regulación del crecimiento tumoral.: Estudio de marcadores de proliferación celular y del complejo proteínas p53/p21WAF1/CIP1/mdm-2
title_fullStr	Cáncer gástrico y regulación del crecimiento tumoral.: Estudio de marcadores de proliferación celular y del complejo proteínas p53/p21WAF1/CIP1/mdm-2
title_full_unstemmed	Cáncer gástrico y regulación del crecimiento tumoral.: Estudio de marcadores de proliferación celular y del complejo proteínas p53/p21WAF1/CIP1/mdm-2
title_sort	cáncer gástrico y regulación del crecimiento tumoral.: estudio de marcadores de proliferación celular y del complejo proteínas p53/p21waf1/cip1/mdm-2
publisher	Sociedad Médica de Santiago
publishDate	2000
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872000000200001
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Cáncer gástrico y regulación del crecimiento tumoral.: Estudio de marcadores de proliferación celular y del complejo proteínas p53/p21WAF1/CIP1/mdm-2

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