Mutación del gen p53 en el cáncer de la vesícula biliar

Background: Gallbladder cancer frequency and mortality renders it one of the most important neoplastic diseases in Chile. P53 tumor suppressor gene has been studied in most types of cancer, but there is scarce information about it in gallbladder cancer. Aim: To study the frequency of P53 gene mutati...

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Autores principales:	Roa E,Iván, Melo,Angélica, Roa S,Juan, Araya O,Juan, Villaseca H,Miguel, de Aretxabala U,Xabier
Lenguaje:	Spanish / Castilian
Publicado:	Sociedad Médica de Santiago 2000
Materias:	Gallbladder neoplasms Genes Suppressor tumor Immunohistochemistry
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872000000300001
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spelling	oai:scielo:S0034-988720000003000012000-06-20Mutación del gen p53 en el cáncer de la vesícula biliarRoa E,IvánMelo,AngélicaRoa S,JuanAraya O,JuanVillaseca H,Miguelde Aretxabala U,Xabier Gallbladder neoplasms Genes Suppressor tumor Immunohistochemistry Background: Gallbladder cancer frequency and mortality renders it one of the most important neoplastic diseases in Chile. P53 tumor suppressor gene has been studied in most types of cancer, but there is scarce information about it in gallbladder cancer. Aim: To study the frequency of P53 gene mutation in gallbladder cancer in the ninth region of Chile. Material and methods: In 25 pathological samples of gallbladder cancer, the direct amplification and sequencing of p53 gene exons 5,6,7,8-8 was possible. Results: Seventeen punctual mutations were observed in 13 cases (52%). There were 10 transitions, 5 transversions, one insertion (codon 194) and one deletion (codon 186). Eight cases had mutations in exon 5, six had mutations in exon 6, two had mutations in exon 7 and one had mutations in exons 8-9. In 14 of 25 cases, gene p53 protein was positive. When immunohistochemical expression of gene p53 protein was positive in more than 20% of cells, there was a high correlation between genetic alterations and immunohistochemical expression of the protein, with a specificity, sensitivity, positive and negative predictive values over 80%. Conclusions: P53 gene mutation is observed in a high proportion of gallbladder cancers at it can be accurately detected with conventional immunohistochemical techniques. The importance of this gene in the genesis of this carcinoma should be determined studying preneoplastic lesions and early carcinomasinfo:eu-repo/semantics/openAccessSociedad Médica de SantiagoRevista médica de Chile v.128 n.3 20002000-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872000000300001es10.4067/S0034-98872000000300001
institution	Scielo Chile
collection	Scielo Chile
language	Spanish / Castilian
topic	Gallbladder neoplasms Genes Suppressor tumor Immunohistochemistry
spellingShingle	Gallbladder neoplasms Genes Suppressor tumor Immunohistochemistry Roa E,Iván Melo,Angélica Roa S,Juan Araya O,Juan Villaseca H,Miguel de Aretxabala U,Xabier Mutación del gen p53 en el cáncer de la vesícula biliar
description	Background: Gallbladder cancer frequency and mortality renders it one of the most important neoplastic diseases in Chile. P53 tumor suppressor gene has been studied in most types of cancer, but there is scarce information about it in gallbladder cancer. Aim: To study the frequency of P53 gene mutation in gallbladder cancer in the ninth region of Chile. Material and methods: In 25 pathological samples of gallbladder cancer, the direct amplification and sequencing of p53 gene exons 5,6,7,8-8 was possible. Results: Seventeen punctual mutations were observed in 13 cases (52%). There were 10 transitions, 5 transversions, one insertion (codon 194) and one deletion (codon 186). Eight cases had mutations in exon 5, six had mutations in exon 6, two had mutations in exon 7 and one had mutations in exons 8-9. In 14 of 25 cases, gene p53 protein was positive. When immunohistochemical expression of gene p53 protein was positive in more than 20% of cells, there was a high correlation between genetic alterations and immunohistochemical expression of the protein, with a specificity, sensitivity, positive and negative predictive values over 80%. Conclusions: P53 gene mutation is observed in a high proportion of gallbladder cancers at it can be accurately detected with conventional immunohistochemical techniques. The importance of this gene in the genesis of this carcinoma should be determined studying preneoplastic lesions and early carcinomas
author	Roa E,Iván Melo,Angélica Roa S,Juan Araya O,Juan Villaseca H,Miguel de Aretxabala U,Xabier
author_facet	Roa E,Iván Melo,Angélica Roa S,Juan Araya O,Juan Villaseca H,Miguel de Aretxabala U,Xabier
author_sort	Roa E,Iván
title	Mutación del gen p53 en el cáncer de la vesícula biliar
title_short	Mutación del gen p53 en el cáncer de la vesícula biliar
title_full	Mutación del gen p53 en el cáncer de la vesícula biliar
title_fullStr	Mutación del gen p53 en el cáncer de la vesícula biliar
title_full_unstemmed	Mutación del gen p53 en el cáncer de la vesícula biliar
title_sort	mutación del gen p53 en el cáncer de la vesícula biliar
publisher	Sociedad Médica de Santiago
publishDate	2000
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872000000300001
work_keys_str_mv	AT roaeivan mutaciondelgenp53enelcancerdelavesiculabiliar AT meloangelica mutaciondelgenp53enelcancerdelavesiculabiliar AT roasjuan mutaciondelgenp53enelcancerdelavesiculabiliar AT arayaojuan mutaciondelgenp53enelcancerdelavesiculabiliar AT villasecahmiguel mutaciondelgenp53enelcancerdelavesiculabiliar AT dearetxabalauxabier mutaciondelgenp53enelcancerdelavesiculabiliar
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Mutación del gen p53 en el cáncer de la vesícula biliar

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