Determinación de la variabilidad en la condensación de la cromatina espermática y alfa-glucosidasa seminal con relación al espermiograma

Background: Sperm functional tests as an addition to semen analysis have been used to study the fertilization ability of spermatozoa. Besides the usual variability of the seminal analysis an individual variability in the results of functional tests has been recently found. Aim: to evaluate in a thre...

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Autores principales:	Sánchez G,Raúl, Peña S,Patricio, Miska,Werner, Henkel,Ralf
Lenguaje:	Spanish / Castilian
Publicado:	Sociedad Médica de Santiago 2000
Materias:	Semen Spermatozoa Sperm count Sperm maturation
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872000000500005
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spelling	oai:scielo:S0034-988720000005000052004-06-22Determinación de la variabilidad en la condensación de la cromatina espermática y alfa-glucosidasa seminal con relación al espermiogramaSánchez G,RaúlPeña S,PatricioMiska,WernerHenkel,Ralf Semen Spermatozoa Sperm count Sperm maturation Background: Sperm functional tests as an addition to semen analysis have been used to study the fertilization ability of spermatozoa. Besides the usual variability of the seminal analysis an individual variability in the results of functional tests has been recently found. Aim: to evaluate in a three months period, the individual variability of sperm parameters and sperm maturation using the chromatin condensation test and epidydime alpha-glucosidase (that allows to discriminate obstructive processes). Material and method: The evaluation was carried out in two donors (12 samples) apparently in good health. One of them presented evident semen analysis alterations (donor 1) and the other was considered normal under the WHO standards (donor 2). Results: The averages for donor 1 were: Sperm count 24x106 sperm/ml (range 10-58x106 sperm/ml), morphology 31.8% (range 30-35%), total motility 33% (range 20-42%), sperm maturation 38% (range 28-78%), a-glucosidase 8.65 (U/ml (range 5-10 (U/ml). The averages for donor 2 were: Sperm count 96x106 sperm/ml (range 50-140x106 sperm/ml), morphology 32.2% (range 30-35%), total motility 69% (range 58-78%), sperm maturation 17% (range 7-30%), a-glucosidase 36.9 (U/ml (range 20-82 (U/ml). Conclusions: These results show that significant variations can be found in the sperm parameters and in seminal plasma a-glucosidase; however these variations are generally maintained at the normal or abnormal ranges for each individual, except the sperm morphology that was constant and with low variation in both donors. The determination of the chromatin condensation in the semen analysis gives an additional information about the grade of sperm maturation and would be of great value for differentiating between sperm samples that show similar morphology values. (Rev Méd Chile 2000; 128: 483-9)info:eu-repo/semantics/openAccessSociedad Médica de SantiagoRevista médica de Chile v.128 n.5 20002000-05-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872000000500005es10.4067/S0034-98872000000500005
institution	Scielo Chile
collection	Scielo Chile
language	Spanish / Castilian
topic	Semen Spermatozoa Sperm count Sperm maturation
spellingShingle	Semen Spermatozoa Sperm count Sperm maturation Sánchez G,Raúl Peña S,Patricio Miska,Werner Henkel,Ralf Determinación de la variabilidad en la condensación de la cromatina espermática y alfa-glucosidasa seminal con relación al espermiograma
description	Background: Sperm functional tests as an addition to semen analysis have been used to study the fertilization ability of spermatozoa. Besides the usual variability of the seminal analysis an individual variability in the results of functional tests has been recently found. Aim: to evaluate in a three months period, the individual variability of sperm parameters and sperm maturation using the chromatin condensation test and epidydime alpha-glucosidase (that allows to discriminate obstructive processes). Material and method: The evaluation was carried out in two donors (12 samples) apparently in good health. One of them presented evident semen analysis alterations (donor 1) and the other was considered normal under the WHO standards (donor 2). Results: The averages for donor 1 were: Sperm count 24x106 sperm/ml (range 10-58x106 sperm/ml), morphology 31.8% (range 30-35%), total motility 33% (range 20-42%), sperm maturation 38% (range 28-78%), a-glucosidase 8.65 (U/ml (range 5-10 (U/ml). The averages for donor 2 were: Sperm count 96x106 sperm/ml (range 50-140x106 sperm/ml), morphology 32.2% (range 30-35%), total motility 69% (range 58-78%), sperm maturation 17% (range 7-30%), a-glucosidase 36.9 (U/ml (range 20-82 (U/ml). Conclusions: These results show that significant variations can be found in the sperm parameters and in seminal plasma a-glucosidase; however these variations are generally maintained at the normal or abnormal ranges for each individual, except the sperm morphology that was constant and with low variation in both donors. The determination of the chromatin condensation in the semen analysis gives an additional information about the grade of sperm maturation and would be of great value for differentiating between sperm samples that show similar morphology values. (Rev Méd Chile 2000; 128: 483-9)
author	Sánchez G,Raúl Peña S,Patricio Miska,Werner Henkel,Ralf
author_facet	Sánchez G,Raúl Peña S,Patricio Miska,Werner Henkel,Ralf
author_sort	Sánchez G,Raúl
title	Determinación de la variabilidad en la condensación de la cromatina espermática y alfa-glucosidasa seminal con relación al espermiograma
title_short	Determinación de la variabilidad en la condensación de la cromatina espermática y alfa-glucosidasa seminal con relación al espermiograma
title_full	Determinación de la variabilidad en la condensación de la cromatina espermática y alfa-glucosidasa seminal con relación al espermiograma
title_fullStr	Determinación de la variabilidad en la condensación de la cromatina espermática y alfa-glucosidasa seminal con relación al espermiograma
title_full_unstemmed	Determinación de la variabilidad en la condensación de la cromatina espermática y alfa-glucosidasa seminal con relación al espermiograma
title_sort	determinación de la variabilidad en la condensación de la cromatina espermática y alfa-glucosidasa seminal con relación al espermiograma
publisher	Sociedad Médica de Santiago
publishDate	2000
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872000000500005
work_keys_str_mv	AT sanchezgraul determinaciondelavariabilidadenlacondensaciondelacromatinaespermaticayalfaglucosidasaseminalconrelacionalespermiograma AT penaspatricio determinaciondelavariabilidadenlacondensaciondelacromatinaespermaticayalfaglucosidasaseminalconrelacionalespermiograma AT miskawerner determinaciondelavariabilidadenlacondensaciondelacromatinaespermaticayalfaglucosidasaseminalconrelacionalespermiograma AT henkelralf determinaciondelavariabilidadenlacondensaciondelacromatinaespermaticayalfaglucosidasaseminalconrelacionalespermiograma
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Determinación de la variabilidad en la condensación de la cromatina espermática y alfa-glucosidasa seminal con relación al espermiograma

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