Contribución del neuropéptido y a la fisiología de la co-transmisión simpática humana.: Estudios en biopsias de vena safena

Background: It is known that the sympathetic varicosities co-store and co-release norepinephrine (NE) together with adenosine S-triphosphate (ATP) and neuropeptide Y (NPY). Aim: To describe the chemical characterisation of stored and released NPY from the varicosities of sympathetic nerve terminals...

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Autores principales:	Donoso G,María Verónica, Miranda J,Ramiro, Irarrázaval Ll,Manuel José, Morán V,Sergio, Zalaquett S,Ricardo, Huidobro-Toro,Juan Pablo
Lenguaje:	Spanish / Castilian
Publicado:	Sociedad Médica de Santiago 2000
Materias:	Biogenic amine neurotransmitters Neuropeptide Y Saphenous vein
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872000000800001
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spelling	oai:scielo:S0034-988720000008000012005-11-29Contribución del neuropéptido y a la fisiología de la co-transmisión simpática humana.: Estudios en biopsias de vena safenaDonoso G,María VerónicaMiranda J,RamiroIrarrázaval Ll,Manuel JoséMorán V,SergioZalaquett S,RicardoHuidobro-Toro,Juan Pablo Biogenic amine neurotransmitters Neuropeptide Y Saphenous vein Background: It is known that the sympathetic varicosities co-store and co-release norepinephrine (NE) together with adenosine S-triphosphate (ATP) and neuropeptide Y (NPY). Aim: To describe the chemical characterisation of stored and released NPY from the varicosities of sympathetic nerve terminals surrounding segments of the human saphenous vein, and the vasomotor activity of rings electrically depolarized or contracted by the exogenous application of the co-transmitters. Material and methods: Saphenous vein tissues were obtained from patients undergoing elective cardiac revascularization surgery. Results: The chromatographic profile of NPY extracted from biopsies is identical to a chemical standard of human NPY. Upon electrical depolarisation of the perivascular sympathetic nerve terminals, we demonstrated the release of NPY to the superfusion media, which did not exceed a 1% of its stored content. The release of the peptide is sensitive to guanethidine, and to extracellular calcium, suggesting that the mechanism of its release is exocytotic in nature. The electrically evoked release of NPY is dependent on the frequency and duration of the electrical pulses. Phenoxybenzamine reduces the electrically evoked release of NPY. Exogenous application of NE and ATP contract saphenous vein rings; the simultaneous application of NE plus ATP causes a synergic response, effect which is further potentiated by the joint co-application of 10 nM NPY. Conclusions: Present results highlight the role of NPY as a sympathetic co-transmitter in the regulation of human vascular tone. (Rev Méd Chile 2000; 128: 829-38).info:eu-repo/semantics/openAccessSociedad Médica de SantiagoRevista médica de Chile v.128 n.8 20002000-08-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872000000800001es10.4067/S0034-98872000000800001
institution	Scielo Chile
collection	Scielo Chile
language	Spanish / Castilian
topic	Biogenic amine neurotransmitters Neuropeptide Y Saphenous vein
spellingShingle	Biogenic amine neurotransmitters Neuropeptide Y Saphenous vein Donoso G,María Verónica Miranda J,Ramiro Irarrázaval Ll,Manuel José Morán V,Sergio Zalaquett S,Ricardo Huidobro-Toro,Juan Pablo Contribución del neuropéptido y a la fisiología de la co-transmisión simpática humana.: Estudios en biopsias de vena safena
description	Background: It is known that the sympathetic varicosities co-store and co-release norepinephrine (NE) together with adenosine S-triphosphate (ATP) and neuropeptide Y (NPY). Aim: To describe the chemical characterisation of stored and released NPY from the varicosities of sympathetic nerve terminals surrounding segments of the human saphenous vein, and the vasomotor activity of rings electrically depolarized or contracted by the exogenous application of the co-transmitters. Material and methods: Saphenous vein tissues were obtained from patients undergoing elective cardiac revascularization surgery. Results: The chromatographic profile of NPY extracted from biopsies is identical to a chemical standard of human NPY. Upon electrical depolarisation of the perivascular sympathetic nerve terminals, we demonstrated the release of NPY to the superfusion media, which did not exceed a 1% of its stored content. The release of the peptide is sensitive to guanethidine, and to extracellular calcium, suggesting that the mechanism of its release is exocytotic in nature. The electrically evoked release of NPY is dependent on the frequency and duration of the electrical pulses. Phenoxybenzamine reduces the electrically evoked release of NPY. Exogenous application of NE and ATP contract saphenous vein rings; the simultaneous application of NE plus ATP causes a synergic response, effect which is further potentiated by the joint co-application of 10 nM NPY. Conclusions: Present results highlight the role of NPY as a sympathetic co-transmitter in the regulation of human vascular tone. (Rev Méd Chile 2000; 128: 829-38).
author	Donoso G,María Verónica Miranda J,Ramiro Irarrázaval Ll,Manuel José Morán V,Sergio Zalaquett S,Ricardo Huidobro-Toro,Juan Pablo
author_facet	Donoso G,María Verónica Miranda J,Ramiro Irarrázaval Ll,Manuel José Morán V,Sergio Zalaquett S,Ricardo Huidobro-Toro,Juan Pablo
author_sort	Donoso G,María Verónica
title	Contribución del neuropéptido y a la fisiología de la co-transmisión simpática humana.: Estudios en biopsias de vena safena
title_short	Contribución del neuropéptido y a la fisiología de la co-transmisión simpática humana.: Estudios en biopsias de vena safena
title_full	Contribución del neuropéptido y a la fisiología de la co-transmisión simpática humana.: Estudios en biopsias de vena safena
title_fullStr	Contribución del neuropéptido y a la fisiología de la co-transmisión simpática humana.: Estudios en biopsias de vena safena
title_full_unstemmed	Contribución del neuropéptido y a la fisiología de la co-transmisión simpática humana.: Estudios en biopsias de vena safena
title_sort	contribución del neuropéptido y a la fisiología de la co-transmisión simpática humana.: estudios en biopsias de vena safena
publisher	Sociedad Médica de Santiago
publishDate	2000
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872000000800001
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Contribución del neuropéptido y a la fisiología de la co-transmisión simpática humana.: Estudios en biopsias de vena safena

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