Presencia de integrones y su relación con la resistencia a cefalosporinas de tercera generación en cepas de Acinetobacter baumannii de origen nosocomial

Background: Acinetobacter baumannii is an important etiological agent causing nosocomial infections. High level of resistance for different kind of antimicrobials has been observed, including ß-lactam antibiotics. This feature, chromosomal or plasmid encoded, has been associated to integrons harbour...

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Autores principales:	Ramírez G,César, Pino I,Carolina, González R,Gerardo, Bello T,Helia, Domínguez Y,Mariana, Mella M,Sergio, Zemelman Z,Raúl, K Young,Hilary, GB Amyes,Sebastian
Lenguaje:	Spanish / Castilian
Publicado:	Sociedad Médica de Santiago 2000
Materias:	Acinetobacter infections Antibiotics, lactam Cephalosporins Drug resistance, microbial
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872000000800005
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spelling	oai:scielo:S0034-988720000008000052005-11-29Presencia de integrones y su relación con la resistencia a cefalosporinas de tercera generación en cepas de Acinetobacter baumannii de origen nosocomialRamírez G,CésarPino I,CarolinaGonzález R,GerardoBello T,HeliaDomínguez Y,MarianaMella M,SergioZemelman Z,RaúlK Young,HilaryGB Amyes,Sebastian Acinetobacter infections Antibiotics, lactam Cephalosporins Drug resistance, microbial Background: Acinetobacter baumannii is an important etiological agent causing nosocomial infections. High level of resistance for different kind of antimicrobials has been observed, including ß-lactam antibiotics. This feature, chromosomal or plasmid encoded, has been associated to integrons harbouring antibiotic resistance gene cassettes. Aims: To investigate the presence of integrons among clinical isolates resistant to third generation cephalosporins (3GC). Material and methods: One hundred A. baumannii strains isolated from several Chilean hospitals were included in this study. Minimal inhibitory concentrations (MIC) of 3GC by an agar dilution method were carried out. Integrons class 1, 2 and 3 were investigated by colony blot hybridisation and confirmed by PCR. Results: High level of resistance to all assayed 3GC was observed. On the other hand, integrón class 2 was the most prevalent (77% of isolates) followed by integron class 1 (52%). Forty six percent of isolates hybridised with probes for both of them. However, no positive hybridisation was detected for integron class 3. Conclusions: Nevertheless, most isolates harboured one or both class of integron; there was no direct relationship between the presence of these genetic structures and the resistance to this kind of ß-lactam antibiotics. (Rev Méd Chile 2000; 128: 863-7 ).info:eu-repo/semantics/openAccessSociedad Médica de SantiagoRevista médica de Chile v.128 n.8 20002000-08-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872000000800005es10.4067/S0034-98872000000800005
institution	Scielo Chile
collection	Scielo Chile
language	Spanish / Castilian
topic	Acinetobacter infections Antibiotics, lactam Cephalosporins Drug resistance, microbial
spellingShingle	Acinetobacter infections Antibiotics, lactam Cephalosporins Drug resistance, microbial Ramírez G,César Pino I,Carolina González R,Gerardo Bello T,Helia Domínguez Y,Mariana Mella M,Sergio Zemelman Z,Raúl K Young,Hilary GB Amyes,Sebastian Presencia de integrones y su relación con la resistencia a cefalosporinas de tercera generación en cepas de Acinetobacter baumannii de origen nosocomial
description	Background: Acinetobacter baumannii is an important etiological agent causing nosocomial infections. High level of resistance for different kind of antimicrobials has been observed, including ß-lactam antibiotics. This feature, chromosomal or plasmid encoded, has been associated to integrons harbouring antibiotic resistance gene cassettes. Aims: To investigate the presence of integrons among clinical isolates resistant to third generation cephalosporins (3GC). Material and methods: One hundred A. baumannii strains isolated from several Chilean hospitals were included in this study. Minimal inhibitory concentrations (MIC) of 3GC by an agar dilution method were carried out. Integrons class 1, 2 and 3 were investigated by colony blot hybridisation and confirmed by PCR. Results: High level of resistance to all assayed 3GC was observed. On the other hand, integrón class 2 was the most prevalent (77% of isolates) followed by integron class 1 (52%). Forty six percent of isolates hybridised with probes for both of them. However, no positive hybridisation was detected for integron class 3. Conclusions: Nevertheless, most isolates harboured one or both class of integron; there was no direct relationship between the presence of these genetic structures and the resistance to this kind of ß-lactam antibiotics. (Rev Méd Chile 2000; 128: 863-7 ).
author	Ramírez G,César Pino I,Carolina González R,Gerardo Bello T,Helia Domínguez Y,Mariana Mella M,Sergio Zemelman Z,Raúl K Young,Hilary GB Amyes,Sebastian
author_facet	Ramírez G,César Pino I,Carolina González R,Gerardo Bello T,Helia Domínguez Y,Mariana Mella M,Sergio Zemelman Z,Raúl K Young,Hilary GB Amyes,Sebastian
author_sort	Ramírez G,César
title	Presencia de integrones y su relación con la resistencia a cefalosporinas de tercera generación en cepas de Acinetobacter baumannii de origen nosocomial
title_short	Presencia de integrones y su relación con la resistencia a cefalosporinas de tercera generación en cepas de Acinetobacter baumannii de origen nosocomial
title_full	Presencia de integrones y su relación con la resistencia a cefalosporinas de tercera generación en cepas de Acinetobacter baumannii de origen nosocomial
title_fullStr	Presencia de integrones y su relación con la resistencia a cefalosporinas de tercera generación en cepas de Acinetobacter baumannii de origen nosocomial
title_full_unstemmed	Presencia de integrones y su relación con la resistencia a cefalosporinas de tercera generación en cepas de Acinetobacter baumannii de origen nosocomial
title_sort	presencia de integrones y su relación con la resistencia a cefalosporinas de tercera generación en cepas de acinetobacter baumannii de origen nosocomial
publisher	Sociedad Médica de Santiago
publishDate	2000
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872000000800005
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Presencia de integrones y su relación con la resistencia a cefalosporinas de tercera generación en cepas de Acinetobacter baumannii de origen nosocomial

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