Búsqueda de la espiroqueta Borrelia burgdorferi sensu lato mediante PCR en garrapatas ixoideas chilenas silvestres

Background: Lyme disease is a tick-borne human disease caused by the spirochete Borrelia burgdorferi. Main vectors of Lyme disease are ticks of the Ixodes and Amblyomma genera. Cases with clinical manifestations of Lyme disease and favorable responses to antimicrobial agents have been reported in Ch...

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Autor principal:	Osorio A,Gonzalo
Lenguaje:	Spanish / Castilian
Publicado:	Sociedad Médica de Santiago 2001
Materias:	Borrelia burgdorferi Lyme disease Tick-home diseases
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872001000300006
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id	oai:scielo:S0034-98872001000300006
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spelling	oai:scielo:S0034-988720010003000062001-05-22Búsqueda de la espiroqueta Borrelia burgdorferi sensu lato mediante PCR en garrapatas ixoideas chilenas silvestresOsorio A,Gonzalo Borrelia burgdorferi Lyme disease Tick-home diseases Background: Lyme disease is a tick-borne human disease caused by the spirochete Borrelia burgdorferi. Main vectors of Lyme disease are ticks of the Ixodes and Amblyomma genera. Cases with clinical manifestations of Lyme disease and favorable responses to antimicrobial agents have been reported in Chile, some of them with erythema migrans, the hallmark of B burgdorferi infection. Aim: To detect the presence of B burgdorferi in Chilean ticks. Material and methods: A total of 62 ticks were recollected from wild rodents and cervidae in the Southern region of Chile. Infected and non infected ticks of the species Ixodes ricimus, were used as controls. Insects were homogenised and B burgdorferi was detected using classical and nested polymerase chain reactions. Results: B burgdorferi was not detected in the studied ticks. Conclusions: Although all the elements required for the enzootic cycle of B burgdorferi are present in Chile, its direct detection in Chilean ticks using the nested polymerase chain reaction assay was negative. (Rev Méd Chile 2001; 129: 270-6).info:eu-repo/semantics/openAccessSociedad Médica de SantiagoRevista médica de Chile v.129 n.3 20012001-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872001000300006es10.4067/S0034-98872001000300006
institution	Scielo Chile
collection	Scielo Chile
language	Spanish / Castilian
topic	Borrelia burgdorferi Lyme disease Tick-home diseases
spellingShingle	Borrelia burgdorferi Lyme disease Tick-home diseases Osorio A,Gonzalo Búsqueda de la espiroqueta Borrelia burgdorferi sensu lato mediante PCR en garrapatas ixoideas chilenas silvestres
description	Background: Lyme disease is a tick-borne human disease caused by the spirochete Borrelia burgdorferi. Main vectors of Lyme disease are ticks of the Ixodes and Amblyomma genera. Cases with clinical manifestations of Lyme disease and favorable responses to antimicrobial agents have been reported in Chile, some of them with erythema migrans, the hallmark of B burgdorferi infection. Aim: To detect the presence of B burgdorferi in Chilean ticks. Material and methods: A total of 62 ticks were recollected from wild rodents and cervidae in the Southern region of Chile. Infected and non infected ticks of the species Ixodes ricimus, were used as controls. Insects were homogenised and B burgdorferi was detected using classical and nested polymerase chain reactions. Results: B burgdorferi was not detected in the studied ticks. Conclusions: Although all the elements required for the enzootic cycle of B burgdorferi are present in Chile, its direct detection in Chilean ticks using the nested polymerase chain reaction assay was negative. (Rev Méd Chile 2001; 129: 270-6).
author	Osorio A,Gonzalo
author_facet	Osorio A,Gonzalo
author_sort	Osorio A,Gonzalo
title	Búsqueda de la espiroqueta Borrelia burgdorferi sensu lato mediante PCR en garrapatas ixoideas chilenas silvestres
title_short	Búsqueda de la espiroqueta Borrelia burgdorferi sensu lato mediante PCR en garrapatas ixoideas chilenas silvestres
title_full	Búsqueda de la espiroqueta Borrelia burgdorferi sensu lato mediante PCR en garrapatas ixoideas chilenas silvestres
title_fullStr	Búsqueda de la espiroqueta Borrelia burgdorferi sensu lato mediante PCR en garrapatas ixoideas chilenas silvestres
title_full_unstemmed	Búsqueda de la espiroqueta Borrelia burgdorferi sensu lato mediante PCR en garrapatas ixoideas chilenas silvestres
title_sort	búsqueda de la espiroqueta borrelia burgdorferi sensu lato mediante pcr en garrapatas ixoideas chilenas silvestres
publisher	Sociedad Médica de Santiago
publishDate	2001
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872001000300006
work_keys_str_mv	AT osorioagonzalo busquedadelaespiroquetaborreliaburgdorferisensulatomediantepcrengarrapatasixoideaschilenassilvestres
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Búsqueda de la espiroqueta Borrelia burgdorferi sensu lato mediante PCR en garrapatas ixoideas chilenas silvestres

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