Efecto de enzimas proteolíticas y de la abstinencia sexual en la cuantificación bioquímica de alfa-glucosidasa en plasma seminal humano

Background: alpha-glucosidase is found in human seminal plasma as an acid form, located in accessory glands, and as a neutral form secreted almost exclusively by the epididymis. Quantification of alpha-glucosidase activity is a marker of the secretory function of the epididymis and indemnity of the...

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Autores principales:	Peña S,Patricio, Sánchez G,Raúl, Vásquez P,Bélgica, Castillo K,Claudia, Miska,Werner
Lenguaje:	Spanish / Castilian
Publicado:	Sociedad Médica de Santiago 2001
Materias:	Epididymis Glucosidases Semen Semen preservation
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872001000500003
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spelling	oai:scielo:S0034-988720010005000032005-11-24Efecto de enzimas proteolíticas y de la abstinencia sexual en la cuantificación bioquímica de alfa-glucosidasa en plasma seminal humanoPeña S,PatricioSánchez G,RaúlVásquez P,BélgicaCastillo K,ClaudiaMiska,Werner Epididymis Glucosidases Semen Semen preservation Background: alpha-glucosidase is found in human seminal plasma as an acid form, located in accessory glands, and as a neutral form secreted almost exclusively by the epididymis. Quantification of alpha-glucosidase activity is a marker of the secretory function of the epididymis and indemnity of the sperm transport pathway Aim: To obtain reference values for alpha-glucosidase in normal samples of seminal plasma, to evaluate its behavior in serial samples and to determine the effect of proteolytic enzymes. Material and methods: Fifty donors, with normal semen analysis according to the criteria of the World Health Organization, were evaluated. For the study with alpha-quimotrypsin, 0.1 to 10 mg/ml of the enzyme was added to the seminal plasma from a group of donors. alpha-glucosidase was also measured in semen obtained from nine patients at different time intervals. Results: Normal alpha-glucosidase values ranged from 14.52 to 25.69 µU/ml. Concentrations up to 10 mg/ml of alpha-quimotrypsin (10 times of that usually used in the liquefaction of the semen) did not alter the quantification of alpha-glucosidase. Serial determinations revealed oscillations in their magnitude, which stayed in each patient's characteristic range. However a subgroup presented a marked reduction of the activity of a-glucosidase as the abstinence diminished (40%). Conclusions: Evaluation of alpha-glucosidase in seminal plasma gives reliable information of the secretor state of the epididymis and especially replaces invasive methods used to evaluate the indemnity of the spermatic transport from the epididymis to the anterior urethra. (Rev Méd Chile 2001; 129: 489-93)info:eu-repo/semantics/openAccessSociedad Médica de SantiagoRevista médica de Chile v.129 n.5 20012001-05-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872001000500003es10.4067/S0034-98872001000500003
institution	Scielo Chile
collection	Scielo Chile
language	Spanish / Castilian
topic	Epididymis Glucosidases Semen Semen preservation
spellingShingle	Epididymis Glucosidases Semen Semen preservation Peña S,Patricio Sánchez G,Raúl Vásquez P,Bélgica Castillo K,Claudia Miska,Werner Efecto de enzimas proteolíticas y de la abstinencia sexual en la cuantificación bioquímica de alfa-glucosidasa en plasma seminal humano
description	Background: alpha-glucosidase is found in human seminal plasma as an acid form, located in accessory glands, and as a neutral form secreted almost exclusively by the epididymis. Quantification of alpha-glucosidase activity is a marker of the secretory function of the epididymis and indemnity of the sperm transport pathway Aim: To obtain reference values for alpha-glucosidase in normal samples of seminal plasma, to evaluate its behavior in serial samples and to determine the effect of proteolytic enzymes. Material and methods: Fifty donors, with normal semen analysis according to the criteria of the World Health Organization, were evaluated. For the study with alpha-quimotrypsin, 0.1 to 10 mg/ml of the enzyme was added to the seminal plasma from a group of donors. alpha-glucosidase was also measured in semen obtained from nine patients at different time intervals. Results: Normal alpha-glucosidase values ranged from 14.52 to 25.69 µU/ml. Concentrations up to 10 mg/ml of alpha-quimotrypsin (10 times of that usually used in the liquefaction of the semen) did not alter the quantification of alpha-glucosidase. Serial determinations revealed oscillations in their magnitude, which stayed in each patient's characteristic range. However a subgroup presented a marked reduction of the activity of a-glucosidase as the abstinence diminished (40%). Conclusions: Evaluation of alpha-glucosidase in seminal plasma gives reliable information of the secretor state of the epididymis and especially replaces invasive methods used to evaluate the indemnity of the spermatic transport from the epididymis to the anterior urethra. (Rev Méd Chile 2001; 129: 489-93)
author	Peña S,Patricio Sánchez G,Raúl Vásquez P,Bélgica Castillo K,Claudia Miska,Werner
author_facet	Peña S,Patricio Sánchez G,Raúl Vásquez P,Bélgica Castillo K,Claudia Miska,Werner
author_sort	Peña S,Patricio
title	Efecto de enzimas proteolíticas y de la abstinencia sexual en la cuantificación bioquímica de alfa-glucosidasa en plasma seminal humano
title_short	Efecto de enzimas proteolíticas y de la abstinencia sexual en la cuantificación bioquímica de alfa-glucosidasa en plasma seminal humano
title_full	Efecto de enzimas proteolíticas y de la abstinencia sexual en la cuantificación bioquímica de alfa-glucosidasa en plasma seminal humano
title_fullStr	Efecto de enzimas proteolíticas y de la abstinencia sexual en la cuantificación bioquímica de alfa-glucosidasa en plasma seminal humano
title_full_unstemmed	Efecto de enzimas proteolíticas y de la abstinencia sexual en la cuantificación bioquímica de alfa-glucosidasa en plasma seminal humano
title_sort	efecto de enzimas proteolíticas y de la abstinencia sexual en la cuantificación bioquímica de alfa-glucosidasa en plasma seminal humano
publisher	Sociedad Médica de Santiago
publishDate	2001
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872001000500003
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Efecto de enzimas proteolíticas y de la abstinencia sexual en la cuantificación bioquímica de alfa-glucosidasa en plasma seminal humano

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