Helicobacter pylori: análisis de cagA y genotipificación de vacA en Chile. Detección de una cepa s2/m1

Background: The genes cagA and vacA encode H pylori virulence factors. Aim: To genotype these genes in H pylori strains isolated from patients with upper gastrointestinal symptoms. Material and methods: We studied 50 patients who underwent an upper gastrointestinal endoscopy, with positive culture f...

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Autores principales:	Martínez T,Alejandra, González C,Carlos, Kawaguchi P,Fernando, Montoya,Rolando, Corvalán,Alejandro, Madariaga B,Jaime, Roa S,Jorge, García C,Apolinaria, Salgado,Fernando, Solar,Henry, Palma,Mariana
Lenguaje:	Spanish / Castilian
Publicado:	Sociedad Médica de Santiago 2001
Materias:	Helicobacter pylori Genotype Genoma, bacterial
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872001001000006
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spelling	oai:scielo:S0034-988720010010000062005-11-14Helicobacter pylori: análisis de cagA y genotipificación de vacA en Chile. Detección de una cepa s2/m1Martínez T,AlejandraGonzález C,CarlosKawaguchi P,FernandoMontoya,RolandoCorvalán,AlejandroMadariaga B,JaimeRoa S,JorgeGarcía C,ApolinariaSalgado,FernandoSolar,HenryPalma,Mariana Helicobacter pylori Genotype Genoma, bacterial Background: The genes cagA and vacA encode H pylori virulence factors. Aim: To genotype these genes in H pylori strains isolated from patients with upper gastrointestinal symptoms. Material and methods: We studied 50 patients who underwent an upper gastrointestinal endoscopy, with positive culture for H pylori. Detection of cagA and vacA gerotyping was done using polymerase chain reactions. Results: The gene cagA was detected in 19 samples (38%). Signal sequences s1 and s2 of vacA gene were detected in 16 samples each (32%). There was simultaneous amplification of s1 and s2 in 6 samples and they were not detected in 9 samples. The middle region of vacA was m1 in 9 samples, m2 in 29 samples and there was simultaneous amplification of m1 and m2 in 12 samples. In 16 samples (32%), more than one type of signal sequence or medial region was detected. Of those patients in whom vacA was the only genotype detected, 15 were s2/m2, 7 were s1/m1, 4 were s1/m2 and 1 was s2/m1. Conclusions: In these patients, the infection with cagA- H pylori strains, predominates, the prevalence of infection with s1 or s2 strains is similar and the predominant medial region is m2 (Rev Méd Chile 2001; 129: 1147-53info:eu-repo/semantics/openAccessSociedad Médica de SantiagoRevista médica de Chile v.129 n.10 20012001-10-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872001001000006es10.4067/S0034-98872001001000006
institution	Scielo Chile
collection	Scielo Chile
language	Spanish / Castilian
topic	Helicobacter pylori Genotype Genoma, bacterial
spellingShingle	Helicobacter pylori Genotype Genoma, bacterial Martínez T,Alejandra González C,Carlos Kawaguchi P,Fernando Montoya,Rolando Corvalán,Alejandro Madariaga B,Jaime Roa S,Jorge García C,Apolinaria Salgado,Fernando Solar,Henry Palma,Mariana Helicobacter pylori: análisis de cagA y genotipificación de vacA en Chile. Detección de una cepa s2/m1
description	Background: The genes cagA and vacA encode H pylori virulence factors. Aim: To genotype these genes in H pylori strains isolated from patients with upper gastrointestinal symptoms. Material and methods: We studied 50 patients who underwent an upper gastrointestinal endoscopy, with positive culture for H pylori. Detection of cagA and vacA gerotyping was done using polymerase chain reactions. Results: The gene cagA was detected in 19 samples (38%). Signal sequences s1 and s2 of vacA gene were detected in 16 samples each (32%). There was simultaneous amplification of s1 and s2 in 6 samples and they were not detected in 9 samples. The middle region of vacA was m1 in 9 samples, m2 in 29 samples and there was simultaneous amplification of m1 and m2 in 12 samples. In 16 samples (32%), more than one type of signal sequence or medial region was detected. Of those patients in whom vacA was the only genotype detected, 15 were s2/m2, 7 were s1/m1, 4 were s1/m2 and 1 was s2/m1. Conclusions: In these patients, the infection with cagA- H pylori strains, predominates, the prevalence of infection with s1 or s2 strains is similar and the predominant medial region is m2 (Rev Méd Chile 2001; 129: 1147-53
author	Martínez T,Alejandra González C,Carlos Kawaguchi P,Fernando Montoya,Rolando Corvalán,Alejandro Madariaga B,Jaime Roa S,Jorge García C,Apolinaria Salgado,Fernando Solar,Henry Palma,Mariana
author_facet	Martínez T,Alejandra González C,Carlos Kawaguchi P,Fernando Montoya,Rolando Corvalán,Alejandro Madariaga B,Jaime Roa S,Jorge García C,Apolinaria Salgado,Fernando Solar,Henry Palma,Mariana
author_sort	Martínez T,Alejandra
title	Helicobacter pylori: análisis de cagA y genotipificación de vacA en Chile. Detección de una cepa s2/m1
title_short	Helicobacter pylori: análisis de cagA y genotipificación de vacA en Chile. Detección de una cepa s2/m1
title_full	Helicobacter pylori: análisis de cagA y genotipificación de vacA en Chile. Detección de una cepa s2/m1
title_fullStr	Helicobacter pylori: análisis de cagA y genotipificación de vacA en Chile. Detección de una cepa s2/m1
title_full_unstemmed	Helicobacter pylori: análisis de cagA y genotipificación de vacA en Chile. Detección de una cepa s2/m1
title_sort	helicobacter pylori: análisis de caga y genotipificación de vaca en chile. detección de una cepa s2/m1
publisher	Sociedad Médica de Santiago
publishDate	2001
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872001001000006
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Helicobacter pylori: análisis de cagA y genotipificación de vacA en Chile. Detección de una cepa s2/m1

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