El colágeno de la reestenosis post angioplastia con stent: ¿se origina en la íntima o en la adventicia?

Background: Restenosis post stenting is due to the deposit of extracellular matrix, mainly collagen in the neointima. Controversy exists regarding if collagen is generated locally or by immigration from the adventitia. Aim: To study the fibrocellular response after stent implantation in rabbit iliac...

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Autores principales:	Guarda S,Eduardo, Fajuri N,Alejandro, Martínez S,Alejandro, Marchant D,Eugenio, Vecchiola C,Andrea, Valenzuela S,Edith, Lazen V,Rosa
Lenguaje:	Spanish / Castilian
Publicado:	Sociedad Médica de Santiago 2001
Materias:	Angioplasty Arterial occlusive diseases Collagen
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872001001100002
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spelling	oai:scielo:S0034-988720010011000022002-06-12El colágeno de la reestenosis post angioplastia con stent: ¿se origina en la íntima o en la adventicia?Guarda S,EduardoFajuri N,AlejandroMartínez S,AlejandroMarchant D,EugenioVecchiola C,AndreaValenzuela S,EdithLazen V,Rosa Angioplasty Arterial occlusive diseases Collagen Background: Restenosis post stenting is due to the deposit of extracellular matrix, mainly collagen in the neointima. Controversy exists regarding if collagen is generated locally or by immigration from the adventitia. Aim: To study the fibrocellular response after stent implantation in rabbit iliac arteries. To observe, by immunohistochemistry and in situ hybridization, if collagen type I mRNA is expressed in the neointima, in the media or in the adventitia. Material and methods: Thirty eight white rabbits (New Zealand) of 4 kg received an hypercholesterolemic diet during 1 month. After this period, in all but 6 of them, an angioplasty with stent implantation was performed via right carotid artery in both iliac arteries, using a 1:1.3 relationship regarding the reference vessel. Angiograms were performed at day 0, 4, 21, and 40, followed by paraffin fixation of the injured segments, immunohistochemistry for a-actin and in situ hybridization to detect procollagen type I (alpha1R1) mRNA. Results: No hybridization was observed in non injured arteries or at day 0 (n= 6). Expression of a1R1 mRNA was observed in the neointima starting at day 4 after stenting (n= 8). At day 21 (n= 8) hybridization of procollagen type I was not only observed in the neointima, but also in the media, which became equally intense in both areas. At day 40 (n= 6) hybridization was observed similarly in the media and adventitia. Conclusions: In this model, hybridization of procollagen type I started in the neointima, then involved the media and finally the adventitia. This finding might be useful for designing therapies to be delivered locally at the end of an angioplasty to prevent collagen deposition in the neointima (Rev Méd Chile 2001; 129: 1241-7)info:eu-repo/semantics/openAccessSociedad Médica de SantiagoRevista médica de Chile v.129 n.11 20012001-11-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872001001100002es10.4067/S0034-98872001001100002
institution	Scielo Chile
collection	Scielo Chile
language	Spanish / Castilian
topic	Angioplasty Arterial occlusive diseases Collagen
spellingShingle	Angioplasty Arterial occlusive diseases Collagen Guarda S,Eduardo Fajuri N,Alejandro Martínez S,Alejandro Marchant D,Eugenio Vecchiola C,Andrea Valenzuela S,Edith Lazen V,Rosa El colágeno de la reestenosis post angioplastia con stent: ¿se origina en la íntima o en la adventicia?
description	Background: Restenosis post stenting is due to the deposit of extracellular matrix, mainly collagen in the neointima. Controversy exists regarding if collagen is generated locally or by immigration from the adventitia. Aim: To study the fibrocellular response after stent implantation in rabbit iliac arteries. To observe, by immunohistochemistry and in situ hybridization, if collagen type I mRNA is expressed in the neointima, in the media or in the adventitia. Material and methods: Thirty eight white rabbits (New Zealand) of 4 kg received an hypercholesterolemic diet during 1 month. After this period, in all but 6 of them, an angioplasty with stent implantation was performed via right carotid artery in both iliac arteries, using a 1:1.3 relationship regarding the reference vessel. Angiograms were performed at day 0, 4, 21, and 40, followed by paraffin fixation of the injured segments, immunohistochemistry for a-actin and in situ hybridization to detect procollagen type I (alpha1R1) mRNA. Results: No hybridization was observed in non injured arteries or at day 0 (n= 6). Expression of a1R1 mRNA was observed in the neointima starting at day 4 after stenting (n= 8). At day 21 (n= 8) hybridization of procollagen type I was not only observed in the neointima, but also in the media, which became equally intense in both areas. At day 40 (n= 6) hybridization was observed similarly in the media and adventitia. Conclusions: In this model, hybridization of procollagen type I started in the neointima, then involved the media and finally the adventitia. This finding might be useful for designing therapies to be delivered locally at the end of an angioplasty to prevent collagen deposition in the neointima (Rev Méd Chile 2001; 129: 1241-7)
author	Guarda S,Eduardo Fajuri N,Alejandro Martínez S,Alejandro Marchant D,Eugenio Vecchiola C,Andrea Valenzuela S,Edith Lazen V,Rosa
author_facet	Guarda S,Eduardo Fajuri N,Alejandro Martínez S,Alejandro Marchant D,Eugenio Vecchiola C,Andrea Valenzuela S,Edith Lazen V,Rosa
author_sort	Guarda S,Eduardo
title	El colágeno de la reestenosis post angioplastia con stent: ¿se origina en la íntima o en la adventicia?
title_short	El colágeno de la reestenosis post angioplastia con stent: ¿se origina en la íntima o en la adventicia?
title_full	El colágeno de la reestenosis post angioplastia con stent: ¿se origina en la íntima o en la adventicia?
title_fullStr	El colágeno de la reestenosis post angioplastia con stent: ¿se origina en la íntima o en la adventicia?
title_full_unstemmed	El colágeno de la reestenosis post angioplastia con stent: ¿se origina en la íntima o en la adventicia?
title_sort	el colágeno de la reestenosis post angioplastia con stent: ¿se origina en la íntima o en la adventicia?
publisher	Sociedad Médica de Santiago
publishDate	2001
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872001001100002
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El colágeno de la reestenosis post angioplastia con stent: ¿se origina en la íntima o en la adventicia?

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