Metodología clásica y molecular en la identificación de especies de Enterococcus spp

Background: Enterococcus is a bacterial genus with low virulence. However, in the last years, the importance of some enterococcus species as nosocomial pathogens has increased, specially due to their resistance to some antimicrobial. Aim: To identify enterococcus strains using classical biochemical...

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Autores principales:	Sepúlveda A,Marcela, Bello T,Helia, Ruiz C,Mery, Hormazábal F,José, Domínguez Y,Mariana, González R,Gerardo, Mella M,Sergio, Zemelman Z,Raúl
Lenguaje:	Spanish / Castilian
Publicado:	Sociedad Médica de Santiago 2002
Materias:	Enterococcus Enterococcus fæcalis Enterococcus fæcium Polymerase chain reaction
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872002000100006
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id	oai:scielo:S0034-98872002000100006
record_format	dspace
spelling	oai:scielo:S0034-988720020001000062002-04-09Metodología clásica y molecular en la identificación de especies de Enterococcus sppSepúlveda A,MarcelaBello T,HeliaRuiz C,MeryHormazábal F,JoséDomínguez Y,MarianaGonzález R,GerardoMella M,SergioZemelman Z,Raúl Enterococcus Enterococcus fæcalis Enterococcus fæcium Polymerase chain reaction Background: Enterococcus is a bacterial genus with low virulence. However, in the last years, the importance of some enterococcus species as nosocomial pathogens has increased, specially due to their resistance to some antimicrobial. Aim: To identify enterococcus strains using classical biochemical techniques and genomic amplification with Polymerase Chain Reaction (PCR). Material and methods: Three hundred and five enterococcus strains, isolated between 1996 and 1999, from different clinical specimens in hospitals and other centers of the VIIIth Region of Chile, were studied. The isolates were identified, to the species level, according to the scheme proposed by Carvalho et al. Identification of some strains was confirmed by PCR. Results: Eighty nine percent of isolates were identified as E fæcalis, 10.2% as E fæcium and 3.3% as other species. Conclusions: PCR is a fast and promising technique, useful in the identification of Enterococcus species (Rev Méd Chile 2002; 130: 45-49)info:eu-repo/semantics/openAccessSociedad Médica de SantiagoRevista médica de Chile v.130 n.1 20022002-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872002000100006es10.4067/S0034-98872002000100006
institution	Scielo Chile
collection	Scielo Chile
language	Spanish / Castilian
topic	Enterococcus Enterococcus fæcalis Enterococcus fæcium Polymerase chain reaction
spellingShingle	Enterococcus Enterococcus fæcalis Enterococcus fæcium Polymerase chain reaction Sepúlveda A,Marcela Bello T,Helia Ruiz C,Mery Hormazábal F,José Domínguez Y,Mariana González R,Gerardo Mella M,Sergio Zemelman Z,Raúl Metodología clásica y molecular en la identificación de especies de Enterococcus spp
description	Background: Enterococcus is a bacterial genus with low virulence. However, in the last years, the importance of some enterococcus species as nosocomial pathogens has increased, specially due to their resistance to some antimicrobial. Aim: To identify enterococcus strains using classical biochemical techniques and genomic amplification with Polymerase Chain Reaction (PCR). Material and methods: Three hundred and five enterococcus strains, isolated between 1996 and 1999, from different clinical specimens in hospitals and other centers of the VIIIth Region of Chile, were studied. The isolates were identified, to the species level, according to the scheme proposed by Carvalho et al. Identification of some strains was confirmed by PCR. Results: Eighty nine percent of isolates were identified as E fæcalis, 10.2% as E fæcium and 3.3% as other species. Conclusions: PCR is a fast and promising technique, useful in the identification of Enterococcus species (Rev Méd Chile 2002; 130: 45-49)
author	Sepúlveda A,Marcela Bello T,Helia Ruiz C,Mery Hormazábal F,José Domínguez Y,Mariana González R,Gerardo Mella M,Sergio Zemelman Z,Raúl
author_facet	Sepúlveda A,Marcela Bello T,Helia Ruiz C,Mery Hormazábal F,José Domínguez Y,Mariana González R,Gerardo Mella M,Sergio Zemelman Z,Raúl
author_sort	Sepúlveda A,Marcela
title	Metodología clásica y molecular en la identificación de especies de Enterococcus spp
title_short	Metodología clásica y molecular en la identificación de especies de Enterococcus spp
title_full	Metodología clásica y molecular en la identificación de especies de Enterococcus spp
title_fullStr	Metodología clásica y molecular en la identificación de especies de Enterococcus spp
title_full_unstemmed	Metodología clásica y molecular en la identificación de especies de Enterococcus spp
title_sort	metodología clásica y molecular en la identificación de especies de enterococcus spp
publisher	Sociedad Médica de Santiago
publishDate	2002
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872002000100006
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Metodología clásica y molecular en la identificación de especies de Enterococcus spp

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