Prueba de Elisa en deposición para detectar infección por Helicobacter pylori

Background: Infection with Helicobacter pylori is frequent in Chile, and a good test for its diagnosis ideally should be non-invasive, fast, easy and inexpensive. Aim: To report the use of an immunoassay test, that detects antigens of Helicobacter pylori in stools. Material and methods: One hundred...

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Autores principales:	Morales B,Antonio, Hurtado H,Carmen, Madrid S,Ana María, Pimentel S,Cristián, Espinosa P,María Nelly
Lenguaje:	Spanish / Castilian
Publicado:	Sociedad Médica de Santiago 2002
Materias:	Enzyme-linked immunosorbent assay Helicobacter infections Helicobacter pylori Immunoassay
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872002000100008
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id	oai:scielo:S0034-98872002000100008
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spelling	oai:scielo:S0034-988720020001000082002-04-09Prueba de Elisa en deposición para detectar infección por Helicobacter pyloriMorales B,AntonioHurtado H,CarmenMadrid S,Ana MaríaPimentel S,CristiánEspinosa P,María Nelly Enzyme-linked immunosorbent assay Helicobacter infections Helicobacter pylori Immunoassay Background: Infection with Helicobacter pylori is frequent in Chile, and a good test for its diagnosis ideally should be non-invasive, fast, easy and inexpensive. Aim: To report the use of an immunoassay test, that detects antigens of Helicobacter pylori in stools. Material and methods: One hundred and four patients that required an upper gastrointestinal endoscopy were studied. Stool samples were analyzed using an enzymatic immunoassay. These results were compared with those obtained with the urease test and microscopic examination of smears taken from biopsies obtained during the diagnostic endoscopy. Results: In 81 of 83 patients considered to have the infection according to the endoscopic tests, the antigen was also present in the stools. The test was negative in 16 of 21 patients considered to be without the infection. The resulting sensitivity and specificity for the immunoassay was thus 97.6 and 76.2% respectively, with positive and negative predictive values of 92.4 and 88.9%, respectively. Conclusions: These results are in agreement with those reported in the literature. Further studies are needed to define its usefulness in massive screening, follow up after antibiotic treatments or the detection of coccoid forms of Helicobacter pylori (Rev Méd Chile 2002; 130: 61-65)info:eu-repo/semantics/openAccessSociedad Médica de SantiagoRevista médica de Chile v.130 n.1 20022002-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872002000100008es10.4067/S0034-98872002000100008
institution	Scielo Chile
collection	Scielo Chile
language	Spanish / Castilian
topic	Enzyme-linked immunosorbent assay Helicobacter infections Helicobacter pylori Immunoassay
spellingShingle	Enzyme-linked immunosorbent assay Helicobacter infections Helicobacter pylori Immunoassay Morales B,Antonio Hurtado H,Carmen Madrid S,Ana María Pimentel S,Cristián Espinosa P,María Nelly Prueba de Elisa en deposición para detectar infección por Helicobacter pylori
description	Background: Infection with Helicobacter pylori is frequent in Chile, and a good test for its diagnosis ideally should be non-invasive, fast, easy and inexpensive. Aim: To report the use of an immunoassay test, that detects antigens of Helicobacter pylori in stools. Material and methods: One hundred and four patients that required an upper gastrointestinal endoscopy were studied. Stool samples were analyzed using an enzymatic immunoassay. These results were compared with those obtained with the urease test and microscopic examination of smears taken from biopsies obtained during the diagnostic endoscopy. Results: In 81 of 83 patients considered to have the infection according to the endoscopic tests, the antigen was also present in the stools. The test was negative in 16 of 21 patients considered to be without the infection. The resulting sensitivity and specificity for the immunoassay was thus 97.6 and 76.2% respectively, with positive and negative predictive values of 92.4 and 88.9%, respectively. Conclusions: These results are in agreement with those reported in the literature. Further studies are needed to define its usefulness in massive screening, follow up after antibiotic treatments or the detection of coccoid forms of Helicobacter pylori (Rev Méd Chile 2002; 130: 61-65)
author	Morales B,Antonio Hurtado H,Carmen Madrid S,Ana María Pimentel S,Cristián Espinosa P,María Nelly
author_facet	Morales B,Antonio Hurtado H,Carmen Madrid S,Ana María Pimentel S,Cristián Espinosa P,María Nelly
author_sort	Morales B,Antonio
title	Prueba de Elisa en deposición para detectar infección por Helicobacter pylori
title_short	Prueba de Elisa en deposición para detectar infección por Helicobacter pylori
title_full	Prueba de Elisa en deposición para detectar infección por Helicobacter pylori
title_fullStr	Prueba de Elisa en deposición para detectar infección por Helicobacter pylori
title_full_unstemmed	Prueba de Elisa en deposición para detectar infección por Helicobacter pylori
title_sort	prueba de elisa en deposición para detectar infección por helicobacter pylori
publisher	Sociedad Médica de Santiago
publishDate	2002
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872002000100008
work_keys_str_mv	AT moralesbantonio pruebadeelisaendeposicionparadetectarinfeccionporhelicobacterpylori AT hurtadohcarmen pruebadeelisaendeposicionparadetectarinfeccionporhelicobacterpylori AT madridsanamaria pruebadeelisaendeposicionparadetectarinfeccionporhelicobacterpylori AT pimentelscristian pruebadeelisaendeposicionparadetectarinfeccionporhelicobacterpylori AT espinosapmarianelly pruebadeelisaendeposicionparadetectarinfeccionporhelicobacterpylori
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Prueba de Elisa en deposición para detectar infección por Helicobacter pylori

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