Caracterización molecular de mecanismos de resistencia a cloranfenicol en cepas de Shigella flexneri aisladas en niños chilenos con diarrea aguda

Background: Chloramphenicol is one of the therapeutic options for shigellosis, but resistance to this antimicrobial is increasing. Aim: To characterize molecular mechanisms conferring resistance to chloramphenicol (CmR) in Shigella flexneri strains isolated from Chilean children with acute diarrhea....

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Autores principales:	Farfán U,Mauricio, Flores G,Oscar, Navarro G,Niurka, Prado J,Valeria, Mora L,Guido, Toro U,Cecilia
Lenguaje:	Spanish / Castilian
Publicado:	Sociedad Médica de Santiago 2002
Materias:	Chloramphenicol o-acetyl-transferase Chloramphenicol resistance Genes cat Shigella flexneri
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872002000300005
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spelling	oai:scielo:S0034-988720020003000052002-07-24Caracterización molecular de mecanismos de resistencia a cloranfenicol en cepas de Shigella flexneri aisladas en niños chilenos con diarrea agudaFarfán U,MauricioFlores G,OscarNavarro G,NiurkaPrado J,ValeriaMora L,GuidoToro U,Cecilia Chloramphenicol o-acetyl-transferase Chloramphenicol resistance Genes cat Shigella flexneri Background: Chloramphenicol is one of the therapeutic options for shigellosis, but resistance to this antimicrobial is increasing. Aim: To characterize molecular mechanisms conferring resistance to chloramphenicol (CmR) in Shigella flexneri strains isolated from Chilean children with acute diarrhea. Material and methods: Thirty one Shigella filexneri strains, including 22 with the CmR phenotype were analyzed. Strains were tested for antimicrobial susceptibility by plate dilution and for the presence of an internal fragment of the cat gene encoding for chloramphenicol o-acetyl-transferase, by polymerase chain reaction and Southern blot analysis. Results: All CmR strains had a minimal inhibitory concentration over 64 µg/ml and amplified the internal fragment of the cat gene. Southern blot analyses indicated that this gene was located in the bacterial chromosome. Conclusions: Resistance to chloramphenicol in this group of Shigella flexneri strains was mediated by a chromosomally located cat gene (Rev Méd Chile 2002; 130: 275-80)info:eu-repo/semantics/openAccessSociedad Médica de SantiagoRevista médica de Chile v.130 n.3 20022002-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872002000300005es10.4067/S0034-98872002000300005
institution	Scielo Chile
collection	Scielo Chile
language	Spanish / Castilian
topic	Chloramphenicol o-acetyl-transferase Chloramphenicol resistance Genes cat Shigella flexneri
spellingShingle	Chloramphenicol o-acetyl-transferase Chloramphenicol resistance Genes cat Shigella flexneri Farfán U,Mauricio Flores G,Oscar Navarro G,Niurka Prado J,Valeria Mora L,Guido Toro U,Cecilia Caracterización molecular de mecanismos de resistencia a cloranfenicol en cepas de Shigella flexneri aisladas en niños chilenos con diarrea aguda
description	Background: Chloramphenicol is one of the therapeutic options for shigellosis, but resistance to this antimicrobial is increasing. Aim: To characterize molecular mechanisms conferring resistance to chloramphenicol (CmR) in Shigella flexneri strains isolated from Chilean children with acute diarrhea. Material and methods: Thirty one Shigella filexneri strains, including 22 with the CmR phenotype were analyzed. Strains were tested for antimicrobial susceptibility by plate dilution and for the presence of an internal fragment of the cat gene encoding for chloramphenicol o-acetyl-transferase, by polymerase chain reaction and Southern blot analysis. Results: All CmR strains had a minimal inhibitory concentration over 64 µg/ml and amplified the internal fragment of the cat gene. Southern blot analyses indicated that this gene was located in the bacterial chromosome. Conclusions: Resistance to chloramphenicol in this group of Shigella flexneri strains was mediated by a chromosomally located cat gene (Rev Méd Chile 2002; 130: 275-80)
author	Farfán U,Mauricio Flores G,Oscar Navarro G,Niurka Prado J,Valeria Mora L,Guido Toro U,Cecilia
author_facet	Farfán U,Mauricio Flores G,Oscar Navarro G,Niurka Prado J,Valeria Mora L,Guido Toro U,Cecilia
author_sort	Farfán U,Mauricio
title	Caracterización molecular de mecanismos de resistencia a cloranfenicol en cepas de Shigella flexneri aisladas en niños chilenos con diarrea aguda
title_short	Caracterización molecular de mecanismos de resistencia a cloranfenicol en cepas de Shigella flexneri aisladas en niños chilenos con diarrea aguda
title_full	Caracterización molecular de mecanismos de resistencia a cloranfenicol en cepas de Shigella flexneri aisladas en niños chilenos con diarrea aguda
title_fullStr	Caracterización molecular de mecanismos de resistencia a cloranfenicol en cepas de Shigella flexneri aisladas en niños chilenos con diarrea aguda
title_full_unstemmed	Caracterización molecular de mecanismos de resistencia a cloranfenicol en cepas de Shigella flexneri aisladas en niños chilenos con diarrea aguda
title_sort	caracterización molecular de mecanismos de resistencia a cloranfenicol en cepas de shigella flexneri aisladas en niños chilenos con diarrea aguda
publisher	Sociedad Médica de Santiago
publishDate	2002
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872002000300005
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Caracterización molecular de mecanismos de resistencia a cloranfenicol en cepas de Shigella flexneri aisladas en niños chilenos con diarrea aguda

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