Inmunofenotipificación de linfocitos en sangre periférica de pacientes chagásicos crónicos chilenos mediante citometría de flujo

Background: Cellular immune mechanisms of the resistance to infection by T cruzi as well as the pathogenesis of Chagas disease are still controversial. Aim: To quantify and analyse the peripheral blood immune cells from chagasic and non chagasic patients by flow cytometry. Patients and methods: Peri...

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Autores principales:	Sánchez,Gittith, Zulantay,Inés, Gajardo,Marta, Venegas,Juan, Villanueva,Roxana, Pérez,Andrés, Liendo,Federico, Apt,Werner, Solari,Aldo
Lenguaje:	Spanish / Castilian
Publicado:	Sociedad Médica de Santiago 2002
Materias:	CD4-positive T-lymphocytes CD4-CD8 ratio CD8-positive T-lymphocytes Chagas disease Flow cytometr
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872002000400002
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spelling	oai:scielo:S0034-988720020004000022003-01-24Inmunofenotipificación de linfocitos en sangre periférica de pacientes chagásicos crónicos chilenos mediante citometría de flujoSánchez,GittithZulantay,InésGajardo,MartaVenegas,JuanVillanueva,RoxanaPérez,AndrésLiendo,FedericoApt,WernerSolari,Aldo CD4-positive T-lymphocytes CD4-CD8 ratio CD8-positive T-lymphocytes Chagas disease Flow cytometr Background: Cellular immune mechanisms of the resistance to infection by T cruzi as well as the pathogenesis of Chagas disease are still controversial. Aim: To quantify and analyse the peripheral blood immune cells from chagasic and non chagasic patients by flow cytometry. Patients and methods: Peripheral blood samples were taken from 21 individuals seropositive for Chagas disease, under no specific treatment. Control samples from 21 healthy blood donors were also obtained. To quantify immune cells populations by flow cytometry, antibodies against CD3, CD4, CD8, CD16/56, CD45/14, CD19 and HLA-DR markers were used. Results: The percentage of CD8+ cells was low and the CD4+/CD8+ ratio was high in chagasic patients, compared to their non infected counterparts. No statistically significant differences in the number of CD4+, NK, B, CD4+HLADR+ and CD8+HLADR+ cells, were observed within the two groups. Conclusions: These results show that Chilean chronic chagasic patients have lower percentage of CD8+ cells and higher CD4+/CD8+ ratio than non infected individuals (Rev Méd Chile 2002; 130: 363-367info:eu-repo/semantics/openAccessSociedad Médica de SantiagoRevista médica de Chile v.130 n.4 20022002-04-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872002000400002es10.4067/S0034-98872002000400002
institution	Scielo Chile
collection	Scielo Chile
language	Spanish / Castilian
topic	CD4-positive T-lymphocytes CD4-CD8 ratio CD8-positive T-lymphocytes Chagas disease Flow cytometr
spellingShingle	CD4-positive T-lymphocytes CD4-CD8 ratio CD8-positive T-lymphocytes Chagas disease Flow cytometr Sánchez,Gittith Zulantay,Inés Gajardo,Marta Venegas,Juan Villanueva,Roxana Pérez,Andrés Liendo,Federico Apt,Werner Solari,Aldo Inmunofenotipificación de linfocitos en sangre periférica de pacientes chagásicos crónicos chilenos mediante citometría de flujo
description	Background: Cellular immune mechanisms of the resistance to infection by T cruzi as well as the pathogenesis of Chagas disease are still controversial. Aim: To quantify and analyse the peripheral blood immune cells from chagasic and non chagasic patients by flow cytometry. Patients and methods: Peripheral blood samples were taken from 21 individuals seropositive for Chagas disease, under no specific treatment. Control samples from 21 healthy blood donors were also obtained. To quantify immune cells populations by flow cytometry, antibodies against CD3, CD4, CD8, CD16/56, CD45/14, CD19 and HLA-DR markers were used. Results: The percentage of CD8+ cells was low and the CD4+/CD8+ ratio was high in chagasic patients, compared to their non infected counterparts. No statistically significant differences in the number of CD4+, NK, B, CD4+HLADR+ and CD8+HLADR+ cells, were observed within the two groups. Conclusions: These results show that Chilean chronic chagasic patients have lower percentage of CD8+ cells and higher CD4+/CD8+ ratio than non infected individuals (Rev Méd Chile 2002; 130: 363-367
author	Sánchez,Gittith Zulantay,Inés Gajardo,Marta Venegas,Juan Villanueva,Roxana Pérez,Andrés Liendo,Federico Apt,Werner Solari,Aldo
author_facet	Sánchez,Gittith Zulantay,Inés Gajardo,Marta Venegas,Juan Villanueva,Roxana Pérez,Andrés Liendo,Federico Apt,Werner Solari,Aldo
author_sort	Sánchez,Gittith
title	Inmunofenotipificación de linfocitos en sangre periférica de pacientes chagásicos crónicos chilenos mediante citometría de flujo
title_short	Inmunofenotipificación de linfocitos en sangre periférica de pacientes chagásicos crónicos chilenos mediante citometría de flujo
title_full	Inmunofenotipificación de linfocitos en sangre periférica de pacientes chagásicos crónicos chilenos mediante citometría de flujo
title_fullStr	Inmunofenotipificación de linfocitos en sangre periférica de pacientes chagásicos crónicos chilenos mediante citometría de flujo
title_full_unstemmed	Inmunofenotipificación de linfocitos en sangre periférica de pacientes chagásicos crónicos chilenos mediante citometría de flujo
title_sort	inmunofenotipificación de linfocitos en sangre periférica de pacientes chagásicos crónicos chilenos mediante citometría de flujo
publisher	Sociedad Médica de Santiago
publishDate	2002
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872002000400002
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Inmunofenotipificación de linfocitos en sangre periférica de pacientes chagásicos crónicos chilenos mediante citometría de flujo

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