Identificación y susceptibilidad antifúngica de Candida spp aisladas de micosis invasora. Influencia del porcentaje de inhibición del crecimiento para la determinación de CIM

Background: An increase in the frequency of resistant strains to antifungal drugs has been detected in the last decade. Aim: To report the minimal inhibitory concentration (MIC) to amphotericin B, fluconazole, ketoconazole and itraconazole. To compare the MIC obtained with 80% and 50% of growth inhi...

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Autores principales:	Alvarado P,Daniel, Díaz J,María C, Silva,Víctor
Lenguaje:	Spanish / Castilian
Publicado:	Sociedad Médica de Santiago 2002
Materias:	Antifungal agents Candida Mycose
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872002000400009
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spelling	oai:scielo:S0034-988720020004000092003-01-24Identificación y susceptibilidad antifúngica de Candida spp aisladas de micosis invasora. Influencia del porcentaje de inhibición del crecimiento para la determinación de CIMAlvarado P,DanielDíaz J,María CSilva,Víctor Antifungal agents Candida Mycose Background: An increase in the frequency of resistant strains to antifungal drugs has been detected in the last decade. Aim: To report the minimal inhibitory concentration (MIC) to amphotericin B, fluconazole, ketoconazole and itraconazole. To compare the MIC obtained with 80% and 50% of growth inhibition to the azoles. Material and methods: Fifty yeast strains isolated between 1998 and 1999, from 17 adults and 33 children with invasive mycosis were studied. Susceptibility was determined by broth microdilution method with RPMI 1640 plus glucose 2% according to the National Committee for Clinical Laboratory Standards (1997). Results: The most frequently isolated strains were C albicans in 27 cases and C parapsilosis in 12. All isolates were susceptible to amphotericin B. According to the MICs obtained with 80% of inhibition, 12 strains had MICs considered as resistant to azoles. Five strains were resistant both to fluconazole and itraconazole. Considering MICs obtained with 50% of inhibition, only five strains were found resistant to azoles (p <0.05). Using this criterion, only one C glabrata strain was found to be simultaneously resistant to fluconazole and itraconazole. Conclusions: Similar results in the pattern of susceptibility of Candida spp to azoles, to those reported abroad, are obtained when the MIC is calculated using 50% inhibition (Rev Méd Chile 2002; 130: 416-423)info:eu-repo/semantics/openAccessSociedad Médica de SantiagoRevista médica de Chile v.130 n.4 20022002-04-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872002000400009es10.4067/S0034-98872002000400009
institution	Scielo Chile
collection	Scielo Chile
language	Spanish / Castilian
topic	Antifungal agents Candida Mycose
spellingShingle	Antifungal agents Candida Mycose Alvarado P,Daniel Díaz J,María C Silva,Víctor Identificación y susceptibilidad antifúngica de Candida spp aisladas de micosis invasora. Influencia del porcentaje de inhibición del crecimiento para la determinación de CIM
description	Background: An increase in the frequency of resistant strains to antifungal drugs has been detected in the last decade. Aim: To report the minimal inhibitory concentration (MIC) to amphotericin B, fluconazole, ketoconazole and itraconazole. To compare the MIC obtained with 80% and 50% of growth inhibition to the azoles. Material and methods: Fifty yeast strains isolated between 1998 and 1999, from 17 adults and 33 children with invasive mycosis were studied. Susceptibility was determined by broth microdilution method with RPMI 1640 plus glucose 2% according to the National Committee for Clinical Laboratory Standards (1997). Results: The most frequently isolated strains were C albicans in 27 cases and C parapsilosis in 12. All isolates were susceptible to amphotericin B. According to the MICs obtained with 80% of inhibition, 12 strains had MICs considered as resistant to azoles. Five strains were resistant both to fluconazole and itraconazole. Considering MICs obtained with 50% of inhibition, only five strains were found resistant to azoles (p <0.05). Using this criterion, only one C glabrata strain was found to be simultaneously resistant to fluconazole and itraconazole. Conclusions: Similar results in the pattern of susceptibility of Candida spp to azoles, to those reported abroad, are obtained when the MIC is calculated using 50% inhibition (Rev Méd Chile 2002; 130: 416-423)
author	Alvarado P,Daniel Díaz J,María C Silva,Víctor
author_facet	Alvarado P,Daniel Díaz J,María C Silva,Víctor
author_sort	Alvarado P,Daniel
title	Identificación y susceptibilidad antifúngica de Candida spp aisladas de micosis invasora. Influencia del porcentaje de inhibición del crecimiento para la determinación de CIM
title_short	Identificación y susceptibilidad antifúngica de Candida spp aisladas de micosis invasora. Influencia del porcentaje de inhibición del crecimiento para la determinación de CIM
title_full	Identificación y susceptibilidad antifúngica de Candida spp aisladas de micosis invasora. Influencia del porcentaje de inhibición del crecimiento para la determinación de CIM
title_fullStr	Identificación y susceptibilidad antifúngica de Candida spp aisladas de micosis invasora. Influencia del porcentaje de inhibición del crecimiento para la determinación de CIM
title_full_unstemmed	Identificación y susceptibilidad antifúngica de Candida spp aisladas de micosis invasora. Influencia del porcentaje de inhibición del crecimiento para la determinación de CIM
title_sort	identificación y susceptibilidad antifúngica de candida spp aisladas de micosis invasora. influencia del porcentaje de inhibición del crecimiento para la determinación de cim
publisher	Sociedad Médica de Santiago
publishDate	2002
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872002000400009
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Identificación y susceptibilidad antifúngica de Candida spp aisladas de micosis invasora. Influencia del porcentaje de inhibición del crecimiento para la determinación de CIM

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