Evaluación de técnicas moleculares e inmunoenzimáticas para la detección de E coli enterohemorrágico en brotes de toxi-infecciones alimentarias

Background: Enterohemorrhagic Escherichia coli (EHEC), is an emergent pathogen that causes sporadic infections and outbreaks of gastroenteritis associated with consumption of contaminated food products. Because detection of EHEC in diarrhea patients is not routinely performed, infection is most prob...

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Autores principales:	Vidal O,Maricel, Carreño C,Mónica, Vidal A,Roberto, Arellano C,Carolina, Solari G,Verónica, Prado J,Valeria
Lenguaje:	Spanish / Castilian
Publicado:	Sociedad Médica de Santiago 2002
Materias:	DNA probes Enzyme-linked immunosorbent assay Escherichia coli infections Escherichia coli O157
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872002000600001
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spelling	oai:scielo:S0034-988720020006000012014-08-20Evaluación de técnicas moleculares e inmunoenzimáticas para la detección de E coli enterohemorrágico en brotes de toxi-infecciones alimentariasVidal O,MaricelCarreño C,MónicaVidal A,RobertoArellano C,CarolinaSolari G,VerónicaPrado J,Valeria DNA probes Enzyme-linked immunosorbent assay Escherichia coli infections Escherichia coli O157 Background: Enterohemorrhagic Escherichia coli (EHEC), is an emergent pathogen that causes sporadic infections and outbreaks of gastroenteritis associated with consumption of contaminated food products. Because detection of EHEC in diarrhea patients is not routinely performed, infection is most probably underestimated. Aim: To compare three techniques to detect EHEC: Colony hybridization with DNA probes, polymerase chain reaction (PCR) for the detection of stx1 and stx2 genes and immunoenzymatic detection by ELISA (Premier EHEC) of Stx1 and Stx2 toxins. Material and methods: Four outbreaks of food-borne gastroenteritis were studied including 16 patients and 78 strains of E coli. Twenty one (26,9%) strains, hybridized with the stx1 probe, 1 (1,3%) hybridized only with the stx2 probe and 36 (46,1%) with both probes. PCR amplification for cytotoxin genes was observed in 6 strains (7,7%) from the second outbreak studied. The immunoenzimatic assay detected the cytotoxins in 18 (23,0%), of the 78 studied strains. Agreement between probes and ELISA was 44,8%, between PCR and probes 34,7% and 82,4% between ELISA and PCR. Conclusions: These results indicate a variable yield among different EHEC detection techniques. Considering PCR as the gold standard, ELISA technique showed a better sensitivity and specificity than probes (Rev Méd Chile 2002; 130: 603-9)info:eu-repo/semantics/openAccessSociedad Médica de SantiagoRevista médica de Chile v.130 n.6 20022002-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872002000600001es10.4067/S0034-98872002000600001
institution	Scielo Chile
collection	Scielo Chile
language	Spanish / Castilian
topic	DNA probes Enzyme-linked immunosorbent assay Escherichia coli infections Escherichia coli O157
spellingShingle	DNA probes Enzyme-linked immunosorbent assay Escherichia coli infections Escherichia coli O157 Vidal O,Maricel Carreño C,Mónica Vidal A,Roberto Arellano C,Carolina Solari G,Verónica Prado J,Valeria Evaluación de técnicas moleculares e inmunoenzimáticas para la detección de E coli enterohemorrágico en brotes de toxi-infecciones alimentarias
description	Background: Enterohemorrhagic Escherichia coli (EHEC), is an emergent pathogen that causes sporadic infections and outbreaks of gastroenteritis associated with consumption of contaminated food products. Because detection of EHEC in diarrhea patients is not routinely performed, infection is most probably underestimated. Aim: To compare three techniques to detect EHEC: Colony hybridization with DNA probes, polymerase chain reaction (PCR) for the detection of stx1 and stx2 genes and immunoenzymatic detection by ELISA (Premier EHEC) of Stx1 and Stx2 toxins. Material and methods: Four outbreaks of food-borne gastroenteritis were studied including 16 patients and 78 strains of E coli. Twenty one (26,9%) strains, hybridized with the stx1 probe, 1 (1,3%) hybridized only with the stx2 probe and 36 (46,1%) with both probes. PCR amplification for cytotoxin genes was observed in 6 strains (7,7%) from the second outbreak studied. The immunoenzimatic assay detected the cytotoxins in 18 (23,0%), of the 78 studied strains. Agreement between probes and ELISA was 44,8%, between PCR and probes 34,7% and 82,4% between ELISA and PCR. Conclusions: These results indicate a variable yield among different EHEC detection techniques. Considering PCR as the gold standard, ELISA technique showed a better sensitivity and specificity than probes (Rev Méd Chile 2002; 130: 603-9)
author	Vidal O,Maricel Carreño C,Mónica Vidal A,Roberto Arellano C,Carolina Solari G,Verónica Prado J,Valeria
author_facet	Vidal O,Maricel Carreño C,Mónica Vidal A,Roberto Arellano C,Carolina Solari G,Verónica Prado J,Valeria
author_sort	Vidal O,Maricel
title	Evaluación de técnicas moleculares e inmunoenzimáticas para la detección de E coli enterohemorrágico en brotes de toxi-infecciones alimentarias
title_short	Evaluación de técnicas moleculares e inmunoenzimáticas para la detección de E coli enterohemorrágico en brotes de toxi-infecciones alimentarias
title_full	Evaluación de técnicas moleculares e inmunoenzimáticas para la detección de E coli enterohemorrágico en brotes de toxi-infecciones alimentarias
title_fullStr	Evaluación de técnicas moleculares e inmunoenzimáticas para la detección de E coli enterohemorrágico en brotes de toxi-infecciones alimentarias
title_full_unstemmed	Evaluación de técnicas moleculares e inmunoenzimáticas para la detección de E coli enterohemorrágico en brotes de toxi-infecciones alimentarias
title_sort	evaluación de técnicas moleculares e inmunoenzimáticas para la detección de e coli enterohemorrágico en brotes de toxi-infecciones alimentarias
publisher	Sociedad Médica de Santiago
publishDate	2002
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872002000600001
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Evaluación de técnicas moleculares e inmunoenzimáticas para la detección de E coli enterohemorrágico en brotes de toxi-infecciones alimentarias

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