Detección de secuencias del gen BCR-ABL mediante RT-PCR en pacientes con leucemia en la IX Región: Chile

Background: The BCR-ABL fusion gene is the molecular expression of the Philadelphia chromosome. This cytogenetic aberration is the most frequent alteration found in leukemias, which is produced by the translocation t(9;22). Two different fusion proteins are produced depending on the break point (210...

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Autores principales:	Artigas A,Carmen Gloria, Melo A,Angélica, Roa S,Juan Carlos, Páez F,Eduardo, Vittini de R,Cecilia, Arriagada M,Mónica, González O,Luis, Pflaumer F,Erna, Roa E,Iván
Lenguaje:	Spanish / Castilian
Publicado:	Sociedad Médica de Santiago 2002
Materias:	Leukemia Oncogene proteins fusion Philadelphia chromosome Reverse transcriptase polymerase chain reaction
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872002000600004
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spelling	oai:scielo:S0034-988720020006000042014-08-20Detección de secuencias del gen BCR-ABL mediante RT-PCR en pacientes con leucemia en la IX Región: ChileArtigas A,Carmen GloriaMelo A,AngélicaRoa S,Juan CarlosPáez F,EduardoVittini de R,CeciliaArriagada M,MónicaGonzález O,LuisPflaumer F,ErnaRoa E,Iván Leukemia Oncogene proteins fusion Philadelphia chromosome Reverse transcriptase polymerase chain reaction Background: The BCR-ABL fusion gene is the molecular expression of the Philadelphia chromosome. This cytogenetic aberration is the most frequent alteration found in leukemias, which is produced by the translocation t(9;22). Two different fusion proteins are produced depending on the break point (210 kD and 190 kD). The detection of this gene has both diagnostic and prognostic importance, associated with poor prognosis in acute lymphoblastic leukemia (ALL). Aim: To detect BCR-ABL gene sequences in patients with leukemia from the IX Region of Chile. Material and methods: We studied 58 patients: 5 chronic myeloid leukemia (CML), 35 ALL, 15 acute myeloid leukemia (AML) and 3 biphenotypic leukemia. The gene sequences were detected using reverse transcriptase polymerase chain reaction (RT-PCR). Results: BRC-ABL gene sequences were positive in all patients with CML, 2 of 35 ALL (one child and one adult). The remaining patients were negative. We found p210 and p190 co-expression in 2 CML and 1 ALL. Conclusions: Our results are in agreement with other reports. The detection of these and other genetic alterations will allow us to have invaluable diagnostic and prognostic information from our patients with leukemia (Rev Méd Chile 2002; 130: 623-30)info:eu-repo/semantics/openAccessSociedad Médica de SantiagoRevista médica de Chile v.130 n.6 20022002-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872002000600004es10.4067/S0034-98872002000600004
institution	Scielo Chile
collection	Scielo Chile
language	Spanish / Castilian
topic	Leukemia Oncogene proteins fusion Philadelphia chromosome Reverse transcriptase polymerase chain reaction
spellingShingle	Leukemia Oncogene proteins fusion Philadelphia chromosome Reverse transcriptase polymerase chain reaction Artigas A,Carmen Gloria Melo A,Angélica Roa S,Juan Carlos Páez F,Eduardo Vittini de R,Cecilia Arriagada M,Mónica González O,Luis Pflaumer F,Erna Roa E,Iván Detección de secuencias del gen BCR-ABL mediante RT-PCR en pacientes con leucemia en la IX Región: Chile
description	Background: The BCR-ABL fusion gene is the molecular expression of the Philadelphia chromosome. This cytogenetic aberration is the most frequent alteration found in leukemias, which is produced by the translocation t(9;22). Two different fusion proteins are produced depending on the break point (210 kD and 190 kD). The detection of this gene has both diagnostic and prognostic importance, associated with poor prognosis in acute lymphoblastic leukemia (ALL). Aim: To detect BCR-ABL gene sequences in patients with leukemia from the IX Region of Chile. Material and methods: We studied 58 patients: 5 chronic myeloid leukemia (CML), 35 ALL, 15 acute myeloid leukemia (AML) and 3 biphenotypic leukemia. The gene sequences were detected using reverse transcriptase polymerase chain reaction (RT-PCR). Results: BRC-ABL gene sequences were positive in all patients with CML, 2 of 35 ALL (one child and one adult). The remaining patients were negative. We found p210 and p190 co-expression in 2 CML and 1 ALL. Conclusions: Our results are in agreement with other reports. The detection of these and other genetic alterations will allow us to have invaluable diagnostic and prognostic information from our patients with leukemia (Rev Méd Chile 2002; 130: 623-30)
author	Artigas A,Carmen Gloria Melo A,Angélica Roa S,Juan Carlos Páez F,Eduardo Vittini de R,Cecilia Arriagada M,Mónica González O,Luis Pflaumer F,Erna Roa E,Iván
author_facet	Artigas A,Carmen Gloria Melo A,Angélica Roa S,Juan Carlos Páez F,Eduardo Vittini de R,Cecilia Arriagada M,Mónica González O,Luis Pflaumer F,Erna Roa E,Iván
author_sort	Artigas A,Carmen Gloria
title	Detección de secuencias del gen BCR-ABL mediante RT-PCR en pacientes con leucemia en la IX Región: Chile
title_short	Detección de secuencias del gen BCR-ABL mediante RT-PCR en pacientes con leucemia en la IX Región: Chile
title_full	Detección de secuencias del gen BCR-ABL mediante RT-PCR en pacientes con leucemia en la IX Región: Chile
title_fullStr	Detección de secuencias del gen BCR-ABL mediante RT-PCR en pacientes con leucemia en la IX Región: Chile
title_full_unstemmed	Detección de secuencias del gen BCR-ABL mediante RT-PCR en pacientes con leucemia en la IX Región: Chile
title_sort	detección de secuencias del gen bcr-abl mediante rt-pcr en pacientes con leucemia en la ix región: chile
publisher	Sociedad Médica de Santiago
publishDate	2002
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872002000600004
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Detección de secuencias del gen BCR-ABL mediante RT-PCR en pacientes con leucemia en la IX Región: Chile

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