Anticuerpos a proteínas recombinantes Ro60 Kd, Ro52 Kd y La48 Kd, en el síndrome de Sjögren primario: Utilidad de la combinación de métodos analíticos para detectar anticuerpos anti Ro y anti La

Background: The use of new recombinant antigens may increase the sensitivity and specificity of the detection of anti Ro and anti La antibodies in Sjögren's syndrome. Aim: To determine the immune reactivity of sera from patients with Sjögren's syndrome, against fusion recombinant proteins...

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Autores principales:	Aguilera C,Sergio, González B,María Julieta, Pérez R,Paola, Castillo A,Darwin, Gatica R,Héctor
Lenguaje:	Spanish / Castilian
Publicado:	Sociedad Médica de Santiago 2002
Materias:	Antibody specificity Recombinant proteins Sjögren syndrome
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872002000800001
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spelling	oai:scielo:S0034-988720020008000012014-08-21Anticuerpos a proteínas recombinantes Ro60 Kd, Ro52 Kd y La48 Kd, en el síndrome de Sjögren primario: Utilidad de la combinación de métodos analíticos para detectar anticuerpos anti Ro y anti LaAguilera C,SergioGonzález B,María JulietaPérez R,PaolaCastillo A,DarwinGatica R,Héctor Antibody specificity Recombinant proteins Sjögren syndrome Background: The use of new recombinant antigens may increase the sensitivity and specificity of the detection of anti Ro and anti La antibodies in Sjögren's syndrome. Aim: To determine the immune reactivity of sera from patients with Sjögren's syndrome, against fusion recombinant proteins (prf) Ro60 Kd, Ro52 Kd and La48 Kd expressed in E coli and recombinant protein Ro52 Kd, expressed in baculovirus (prb). Material and methods: Serum samples from 46 patients with a diagnosis of Sjögren's syndrome, according to the European criteria of 1997, were studied. Using conventional ELISA assays, 32 patients had positive anti Ro antibodies (group A) and 16 patients had negative anti Ro and anti La antibodies, but had positive antinuclear antibodies or rheumatoid factors (group B). Antibodies against recombinant proteins were measured by ELISA or Western Blot. Results: Reactivity against prf Ro60 was present in 69% of samples from group A patients and in 36% of samples from group B. Reactivity against prf Ro52 was present in 94% of samples from group A and 50% of samples from group B. Reactivity against prb Ro52 was present in 75% of samples from group A and 40% of samples from group B. Reactivity against prf La was present in 78% of samples by ELISA and 97% of samples by Western Blot. In 10 of 14 serum samples from group B patients, there was reactivity against at least one recombinant protein. Conclusions: A high prevalence of reactivity against recombinant Ro and La proteins was detected in serum samples from patients with Sjögren syndrome (Rev Méd Chile 2002; 130: 841-9)info:eu-repo/semantics/openAccessSociedad Médica de SantiagoRevista médica de Chile v.130 n.8 20022002-08-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872002000800001es10.4067/S0034-98872002000800001
institution	Scielo Chile
collection	Scielo Chile
language	Spanish / Castilian
topic	Antibody specificity Recombinant proteins Sjögren syndrome
spellingShingle	Antibody specificity Recombinant proteins Sjögren syndrome Aguilera C,Sergio González B,María Julieta Pérez R,Paola Castillo A,Darwin Gatica R,Héctor Anticuerpos a proteínas recombinantes Ro60 Kd, Ro52 Kd y La48 Kd, en el síndrome de Sjögren primario: Utilidad de la combinación de métodos analíticos para detectar anticuerpos anti Ro y anti La
description	Background: The use of new recombinant antigens may increase the sensitivity and specificity of the detection of anti Ro and anti La antibodies in Sjögren's syndrome. Aim: To determine the immune reactivity of sera from patients with Sjögren's syndrome, against fusion recombinant proteins (prf) Ro60 Kd, Ro52 Kd and La48 Kd expressed in E coli and recombinant protein Ro52 Kd, expressed in baculovirus (prb). Material and methods: Serum samples from 46 patients with a diagnosis of Sjögren's syndrome, according to the European criteria of 1997, were studied. Using conventional ELISA assays, 32 patients had positive anti Ro antibodies (group A) and 16 patients had negative anti Ro and anti La antibodies, but had positive antinuclear antibodies or rheumatoid factors (group B). Antibodies against recombinant proteins were measured by ELISA or Western Blot. Results: Reactivity against prf Ro60 was present in 69% of samples from group A patients and in 36% of samples from group B. Reactivity against prf Ro52 was present in 94% of samples from group A and 50% of samples from group B. Reactivity against prb Ro52 was present in 75% of samples from group A and 40% of samples from group B. Reactivity against prf La was present in 78% of samples by ELISA and 97% of samples by Western Blot. In 10 of 14 serum samples from group B patients, there was reactivity against at least one recombinant protein. Conclusions: A high prevalence of reactivity against recombinant Ro and La proteins was detected in serum samples from patients with Sjögren syndrome (Rev Méd Chile 2002; 130: 841-9)
author	Aguilera C,Sergio González B,María Julieta Pérez R,Paola Castillo A,Darwin Gatica R,Héctor
author_facet	Aguilera C,Sergio González B,María Julieta Pérez R,Paola Castillo A,Darwin Gatica R,Héctor
author_sort	Aguilera C,Sergio
title	Anticuerpos a proteínas recombinantes Ro60 Kd, Ro52 Kd y La48 Kd, en el síndrome de Sjögren primario: Utilidad de la combinación de métodos analíticos para detectar anticuerpos anti Ro y anti La
title_short	Anticuerpos a proteínas recombinantes Ro60 Kd, Ro52 Kd y La48 Kd, en el síndrome de Sjögren primario: Utilidad de la combinación de métodos analíticos para detectar anticuerpos anti Ro y anti La
title_full	Anticuerpos a proteínas recombinantes Ro60 Kd, Ro52 Kd y La48 Kd, en el síndrome de Sjögren primario: Utilidad de la combinación de métodos analíticos para detectar anticuerpos anti Ro y anti La
title_fullStr	Anticuerpos a proteínas recombinantes Ro60 Kd, Ro52 Kd y La48 Kd, en el síndrome de Sjögren primario: Utilidad de la combinación de métodos analíticos para detectar anticuerpos anti Ro y anti La
title_full_unstemmed	Anticuerpos a proteínas recombinantes Ro60 Kd, Ro52 Kd y La48 Kd, en el síndrome de Sjögren primario: Utilidad de la combinación de métodos analíticos para detectar anticuerpos anti Ro y anti La
title_sort	anticuerpos a proteínas recombinantes ro60 kd, ro52 kd y la48 kd, en el síndrome de sjögren primario: utilidad de la combinación de métodos analíticos para detectar anticuerpos anti ro y anti la
publisher	Sociedad Médica de Santiago
publishDate	2002
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872002000800001
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Anticuerpos a proteínas recombinantes Ro60 Kd, Ro52 Kd y La48 Kd, en el síndrome de Sjögren primario: Utilidad de la combinación de métodos analíticos para detectar anticuerpos anti Ro y anti La

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