Inmunodiagnóstico de las infecciones por Strongyloides stercoralis en Chile utilizando la prueba de ELISA

Background: Strongyloides stercoralis is a world wide distributed small intestinal nematode parasite. In immunocompetent individuals S stercoralis can produce asymptomatic infections or a moderate clinical picture of diarrhea, some cases become chronic. In immunocompromised patients, a disseminated...

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Autores principales:	Mercado P,Rubén, Jercic L,María Isabel, Torres H,Patricio, Alcayaga U,Sergio, Martins de Paula,Fabiana, Costa-Cruz,Julia María, Ueta,Marlene T
Lenguaje:	Spanish / Castilian
Publicado:	Sociedad Médica de Santiago 2002
Materias:	Enzyme linked immunosorbent assay Nematode infections Strongyloides stercoralis
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872002001200005
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spelling	oai:scielo:S0034-988720020012000052003-02-05Inmunodiagnóstico de las infecciones por Strongyloides stercoralis en Chile utilizando la prueba de ELISAMercado P,RubénJercic L,María IsabelTorres H,PatricioAlcayaga U,SergioMartins de Paula,FabianaCosta-Cruz,Julia MaríaUeta,Marlene T Enzyme linked immunosorbent assay Nematode infections Strongyloides stercoralis Background: Strongyloides stercoralis is a world wide distributed small intestinal nematode parasite. In immunocompetent individuals S stercoralis can produce asymptomatic infections or a moderate clinical picture of diarrhea, some cases become chronic. In immunocompromised patients, a disseminated disease may appear, sometimes fatal. In Chile, there is little epidemiological information about S stercoralis infections and appropriate diagnostic techniques are usually not used. Aim: To evaluate the yield of an ELISA test for the diagnosis of strongyloidiasis in Chilean patients. Material and methods: Ten serum samples from patients with S stercoralis infections confirmed by a positive stool examination, 66 samples from individuals with other infections by tissue helminthes (24 toxocariasis, 15 trichinellosis, 11 hydatidosis, 12 fascioliasis and 4 cysticercosis), 13 samples from subjects with autoimmune diseases and 49 samples from apparently healthy individuals with a normal eosinophil count, were studied. ELISA antigen was prepared using a filariform larval extract obtained from a murine species of Strongyloides, maintained in laboratory animals. Results: Using 0.33 optical density units as a cut off value, 9 of 10 sera of S stercoralis infected individuals, had a positive ELISA test. No cross reactions were observed with sera of patients with other helminthic infections, autoimmune diseases or in healthy individuals. Thus, specificity, positive and negative predictive values were 100%. Conclusions: The results obtained are similar with those found by other investigators. ELISA test for strongyloidiasis is a useful tool for the diagnosis of clinical cases and for seroepidemiological studies of this nematode infection in Chile (Rev Méd Chile 2002; 130: 1358-64)info:eu-repo/semantics/openAccessSociedad Médica de SantiagoRevista médica de Chile v.130 n.12 20022002-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872002001200005es10.4067/S0034-98872002001200005
institution	Scielo Chile
collection	Scielo Chile
language	Spanish / Castilian
topic	Enzyme linked immunosorbent assay Nematode infections Strongyloides stercoralis
spellingShingle	Enzyme linked immunosorbent assay Nematode infections Strongyloides stercoralis Mercado P,Rubén Jercic L,María Isabel Torres H,Patricio Alcayaga U,Sergio Martins de Paula,Fabiana Costa-Cruz,Julia María Ueta,Marlene T Inmunodiagnóstico de las infecciones por Strongyloides stercoralis en Chile utilizando la prueba de ELISA
description	Background: Strongyloides stercoralis is a world wide distributed small intestinal nematode parasite. In immunocompetent individuals S stercoralis can produce asymptomatic infections or a moderate clinical picture of diarrhea, some cases become chronic. In immunocompromised patients, a disseminated disease may appear, sometimes fatal. In Chile, there is little epidemiological information about S stercoralis infections and appropriate diagnostic techniques are usually not used. Aim: To evaluate the yield of an ELISA test for the diagnosis of strongyloidiasis in Chilean patients. Material and methods: Ten serum samples from patients with S stercoralis infections confirmed by a positive stool examination, 66 samples from individuals with other infections by tissue helminthes (24 toxocariasis, 15 trichinellosis, 11 hydatidosis, 12 fascioliasis and 4 cysticercosis), 13 samples from subjects with autoimmune diseases and 49 samples from apparently healthy individuals with a normal eosinophil count, were studied. ELISA antigen was prepared using a filariform larval extract obtained from a murine species of Strongyloides, maintained in laboratory animals. Results: Using 0.33 optical density units as a cut off value, 9 of 10 sera of S stercoralis infected individuals, had a positive ELISA test. No cross reactions were observed with sera of patients with other helminthic infections, autoimmune diseases or in healthy individuals. Thus, specificity, positive and negative predictive values were 100%. Conclusions: The results obtained are similar with those found by other investigators. ELISA test for strongyloidiasis is a useful tool for the diagnosis of clinical cases and for seroepidemiological studies of this nematode infection in Chile (Rev Méd Chile 2002; 130: 1358-64)
author	Mercado P,Rubén Jercic L,María Isabel Torres H,Patricio Alcayaga U,Sergio Martins de Paula,Fabiana Costa-Cruz,Julia María Ueta,Marlene T
author_facet	Mercado P,Rubén Jercic L,María Isabel Torres H,Patricio Alcayaga U,Sergio Martins de Paula,Fabiana Costa-Cruz,Julia María Ueta,Marlene T
author_sort	Mercado P,Rubén
title	Inmunodiagnóstico de las infecciones por Strongyloides stercoralis en Chile utilizando la prueba de ELISA
title_short	Inmunodiagnóstico de las infecciones por Strongyloides stercoralis en Chile utilizando la prueba de ELISA
title_full	Inmunodiagnóstico de las infecciones por Strongyloides stercoralis en Chile utilizando la prueba de ELISA
title_fullStr	Inmunodiagnóstico de las infecciones por Strongyloides stercoralis en Chile utilizando la prueba de ELISA
title_full_unstemmed	Inmunodiagnóstico de las infecciones por Strongyloides stercoralis en Chile utilizando la prueba de ELISA
title_sort	inmunodiagnóstico de las infecciones por strongyloides stercoralis en chile utilizando la prueba de elisa
publisher	Sociedad Médica de Santiago
publishDate	2002
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872002001200005
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Inmunodiagnóstico de las infecciones por Strongyloides stercoralis en Chile utilizando la prueba de ELISA

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