Epidemiología molecular de un brote de infecciones por Streptococcus pyogenes en una unidad de quemados

Group A Streptococcal (GAS) infections have increased in frequency and severity worldwide. During April 1996, a nosocomial outbreak associated to GAS infections affected seven patients admitted to a pediatric burn unit. The causative organism was likely disseminated from the source patient to anothe...

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Autores principales:	Fica C,Alberto, Fernández O,Jorge, Ebensperger D,Germán, Cona T,Erna, Galanti D,Andrea, Alonso M,Catalina, Ulloa F,María Teresa, Frola M,Ana María, Prat M,Soledad
Lenguaje:	Spanish / Castilian
Publicado:	Sociedad Médica de Santiago 2003
Materias:	Disease outbreaks Molecular probe technics Molecular sequence data
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872003000200003
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spelling	oai:scielo:S0034-988720030002000032005-11-22Epidemiología molecular de un brote de infecciones por Streptococcus pyogenes en una unidad de quemadosFica C,AlbertoFernández O,JorgeEbensperger D,GermánCona T,ErnaGalanti D,AndreaAlonso M,CatalinaUlloa F,María TeresaFrola M,Ana MaríaPrat M,Soledad Disease outbreaks Molecular probe technics Molecular sequence data Group A Streptococcal (GAS) infections have increased in frequency and severity worldwide. During April 1996, a nosocomial outbreak associated to GAS infections affected seven patients admitted to a pediatric burn unit. The causative organism was likely disseminated from the source patient to another child in the emergency room before he was transferred to the burn unit. Patients developed burn infections or invasive disease. One of them died due to a toxic shock syndrome and 3 other lost their skin grafts. Perineal and nasal microbiological surveillance of 42 related health care workers identified only one of them as carrier of S pyogenes. Aim: To report a molecular analysis of an apparently clonal outbreak. Material and methods: The available isolates were analyzed by molecular methods including random amplified polymorphic DNA analysis (RAPD) with 4 different primers, Sma-I pulsed field gel electrophoresis (PFGE) analysis, and speA, speB and speC detection by polymerase chain reaction (PCR). Results: Two phylogenetically distant and sequentially isolated bacterial groups were identified either by RAPD analysis with selected primers or by Smal-PFGE analysis. The first group involved isolates identified in two patients that included the lethal case. The second bacterial group comprised 5 clinical isolates and the perineal and nasal isolates obtained from a health care worker. Only strains belonging to the first group harbored the speA gene and were associated with invasive disease. The second group could be split further in two subgroups according to their speB profile. Conclusions: RAPD analysis with selected primers can reproduce the PFGE-discriminating ability on the epidemiological analysis of GAS infections (Rev Méd Chile 2003; 131: 145-54)info:eu-repo/semantics/openAccessSociedad Médica de SantiagoRevista médica de Chile v.131 n.2 20032003-02-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872003000200003es10.4067/S0034-98872003000200003
institution	Scielo Chile
collection	Scielo Chile
language	Spanish / Castilian
topic	Disease outbreaks Molecular probe technics Molecular sequence data
spellingShingle	Disease outbreaks Molecular probe technics Molecular sequence data Fica C,Alberto Fernández O,Jorge Ebensperger D,Germán Cona T,Erna Galanti D,Andrea Alonso M,Catalina Ulloa F,María Teresa Frola M,Ana María Prat M,Soledad Epidemiología molecular de un brote de infecciones por Streptococcus pyogenes en una unidad de quemados
description	Group A Streptococcal (GAS) infections have increased in frequency and severity worldwide. During April 1996, a nosocomial outbreak associated to GAS infections affected seven patients admitted to a pediatric burn unit. The causative organism was likely disseminated from the source patient to another child in the emergency room before he was transferred to the burn unit. Patients developed burn infections or invasive disease. One of them died due to a toxic shock syndrome and 3 other lost their skin grafts. Perineal and nasal microbiological surveillance of 42 related health care workers identified only one of them as carrier of S pyogenes. Aim: To report a molecular analysis of an apparently clonal outbreak. Material and methods: The available isolates were analyzed by molecular methods including random amplified polymorphic DNA analysis (RAPD) with 4 different primers, Sma-I pulsed field gel electrophoresis (PFGE) analysis, and speA, speB and speC detection by polymerase chain reaction (PCR). Results: Two phylogenetically distant and sequentially isolated bacterial groups were identified either by RAPD analysis with selected primers or by Smal-PFGE analysis. The first group involved isolates identified in two patients that included the lethal case. The second bacterial group comprised 5 clinical isolates and the perineal and nasal isolates obtained from a health care worker. Only strains belonging to the first group harbored the speA gene and were associated with invasive disease. The second group could be split further in two subgroups according to their speB profile. Conclusions: RAPD analysis with selected primers can reproduce the PFGE-discriminating ability on the epidemiological analysis of GAS infections (Rev Méd Chile 2003; 131: 145-54)
author	Fica C,Alberto Fernández O,Jorge Ebensperger D,Germán Cona T,Erna Galanti D,Andrea Alonso M,Catalina Ulloa F,María Teresa Frola M,Ana María Prat M,Soledad
author_facet	Fica C,Alberto Fernández O,Jorge Ebensperger D,Germán Cona T,Erna Galanti D,Andrea Alonso M,Catalina Ulloa F,María Teresa Frola M,Ana María Prat M,Soledad
author_sort	Fica C,Alberto
title	Epidemiología molecular de un brote de infecciones por Streptococcus pyogenes en una unidad de quemados
title_short	Epidemiología molecular de un brote de infecciones por Streptococcus pyogenes en una unidad de quemados
title_full	Epidemiología molecular de un brote de infecciones por Streptococcus pyogenes en una unidad de quemados
title_fullStr	Epidemiología molecular de un brote de infecciones por Streptococcus pyogenes en una unidad de quemados
title_full_unstemmed	Epidemiología molecular de un brote de infecciones por Streptococcus pyogenes en una unidad de quemados
title_sort	epidemiología molecular de un brote de infecciones por streptococcus pyogenes en una unidad de quemados
publisher	Sociedad Médica de Santiago
publishDate	2003
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872003000200003
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Epidemiología molecular de un brote de infecciones por Streptococcus pyogenes en una unidad de quemados

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