Mutación puntual del gen supresor de tumores TP53 en lesiones preneoplásicas y neoplásicas del estómago: cross- sectional study in a high risk region

Background: In the current model for the development of gastric cancer, regions of multifocal atrophic gastritis give rise to intestinal metaplasia, dysplasia and finally, adenocarcinoma. Aim: To study the frequency and characteristics of TP53 gene mutations in preneoplastic and neoplastic lesions o...

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Autores principales:	Araya O,Juan Carlos, Roa S,Juan Carlos, Villaseca H,Miguel Angel, Roa E,Iván, Guzmán G,Pablo, Alvarado C,César
Lenguaje:	Spanish / Castilian
Publicado:	Sociedad Médica de Santiago 2003
Materias:	Adenocarcinoma Genes supressor tumor Metaplasia
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872003000400002
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spelling	oai:scielo:S0034-988720030004000022004-12-13Mutación puntual del gen supresor de tumores TP53 en lesiones preneoplásicas y neoplásicas del estómago: cross- sectional study in a high risk regionAraya O,Juan CarlosRoa S,Juan CarlosVillaseca H,Miguel AngelRoa E,IvánGuzmán G,PabloAlvarado C,César Adenocarcinoma Genes supressor tumor Metaplasia Background: In the current model for the development of gastric cancer, regions of multifocal atrophic gastritis give rise to intestinal metaplasia, dysplasia and finally, adenocarcinoma. Aim: To study the frequency and characteristics of TP53 gene mutations in preneoplastic and neoplastic lesions of the stomach. Material and methods: DNA sequencing of the TP53 gene was performed in 46 patients with gastric carcinoma. Normal mucosa, intestinal metaplasia and invasive adenocarcinoma tissues were obtained by scraping 6-µm histological sections from formalin-fixed and paraffin-embedded tissue. Results: DNA sequencing of exons 5-9 of the TP53 gene demonstrated a mutation in 31% of patients. These findings were seen both in tumoral tissue (13 cases) and in intestinal metaplasia (2 cases). Most mutations were found in exons 5 and 8, and the majority of them were transitions (10 out of 19 mutations). Discussion: Patients with gastric cancer showed a frequency of TP53 mutations similar to that previously communicated in populations with low gastric cancer risk. Moreover, there was a predominance of transitions, genetic alterations that are identified with carcinogenesis associated with N-nitrosamine compounds. Finally, mutations of TP53 gene were detected in areas of intestinal metaplasia (Rev Méd Chile 2003; 131: 359-65).info:eu-repo/semantics/openAccessSociedad Médica de SantiagoRevista médica de Chile v.131 n.4 20032003-04-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872003000400002es10.4067/S0034-98872003000400002
institution	Scielo Chile
collection	Scielo Chile
language	Spanish / Castilian
topic	Adenocarcinoma Genes supressor tumor Metaplasia
spellingShingle	Adenocarcinoma Genes supressor tumor Metaplasia Araya O,Juan Carlos Roa S,Juan Carlos Villaseca H,Miguel Angel Roa E,Iván Guzmán G,Pablo Alvarado C,César Mutación puntual del gen supresor de tumores TP53 en lesiones preneoplásicas y neoplásicas del estómago: cross- sectional study in a high risk region
description	Background: In the current model for the development of gastric cancer, regions of multifocal atrophic gastritis give rise to intestinal metaplasia, dysplasia and finally, adenocarcinoma. Aim: To study the frequency and characteristics of TP53 gene mutations in preneoplastic and neoplastic lesions of the stomach. Material and methods: DNA sequencing of the TP53 gene was performed in 46 patients with gastric carcinoma. Normal mucosa, intestinal metaplasia and invasive adenocarcinoma tissues were obtained by scraping 6-µm histological sections from formalin-fixed and paraffin-embedded tissue. Results: DNA sequencing of exons 5-9 of the TP53 gene demonstrated a mutation in 31% of patients. These findings were seen both in tumoral tissue (13 cases) and in intestinal metaplasia (2 cases). Most mutations were found in exons 5 and 8, and the majority of them were transitions (10 out of 19 mutations). Discussion: Patients with gastric cancer showed a frequency of TP53 mutations similar to that previously communicated in populations with low gastric cancer risk. Moreover, there was a predominance of transitions, genetic alterations that are identified with carcinogenesis associated with N-nitrosamine compounds. Finally, mutations of TP53 gene were detected in areas of intestinal metaplasia (Rev Méd Chile 2003; 131: 359-65).
author	Araya O,Juan Carlos Roa S,Juan Carlos Villaseca H,Miguel Angel Roa E,Iván Guzmán G,Pablo Alvarado C,César
author_facet	Araya O,Juan Carlos Roa S,Juan Carlos Villaseca H,Miguel Angel Roa E,Iván Guzmán G,Pablo Alvarado C,César
author_sort	Araya O,Juan Carlos
title	Mutación puntual del gen supresor de tumores TP53 en lesiones preneoplásicas y neoplásicas del estómago: cross- sectional study in a high risk region
title_short	Mutación puntual del gen supresor de tumores TP53 en lesiones preneoplásicas y neoplásicas del estómago: cross- sectional study in a high risk region
title_full	Mutación puntual del gen supresor de tumores TP53 en lesiones preneoplásicas y neoplásicas del estómago: cross- sectional study in a high risk region
title_fullStr	Mutación puntual del gen supresor de tumores TP53 en lesiones preneoplásicas y neoplásicas del estómago: cross- sectional study in a high risk region
title_full_unstemmed	Mutación puntual del gen supresor de tumores TP53 en lesiones preneoplásicas y neoplásicas del estómago: cross- sectional study in a high risk region
title_sort	mutación puntual del gen supresor de tumores tp53 en lesiones preneoplásicas y neoplásicas del estómago: cross- sectional study in a high risk region
publisher	Sociedad Médica de Santiago
publishDate	2003
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872003000400002
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Mutación puntual del gen supresor de tumores TP53 en lesiones preneoplásicas y neoplásicas del estómago: cross- sectional study in a high risk region

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