Integrones y cassettes genéticos de resistencia a antimicrobianos en cepas de Shigella flexneri

Background: The resistance of Shigella flexneri to antimicrobial agents can be associated to the presence of integrons that may contain and express antimicrobial resistance gene cassettes. Aim: To study antimicrobial resistance and the presence of integrons and antimicrobial gene cassettes in Shigel...

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Autores principales:	Muñoz A,Jeannette, Bello T,Helia, Domínguez Y,Mariana, Mella M,Sergio, Zemelman Z,Raúl, González R,Gerardo
Lenguaje:	Spanish / Castilian
Publicado:	Sociedad Médica de Santiago 2003
Materias:	Drug resistance, microbial Gene library Integrons Shigella flexneri
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872003000700003
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spelling	oai:scielo:S0034-988720030007000032004-12-13Integrones y cassettes genéticos de resistencia a antimicrobianos en cepas de Shigella flexneriMuñoz A,JeannetteBello T,HeliaDomínguez Y,MarianaMella M,SergioZemelman Z,RaúlGonzález R,Gerardo Drug resistance, microbial Gene library Integrons Shigella flexneri Background: The resistance of Shigella flexneri to antimicrobial agents can be associated to the presence of integrons that may contain and express antimicrobial resistance gene cassettes. Aim: To study antimicrobial resistance and the presence of integrons and antimicrobial gene cassettes in Shigella flexneri strains. Material and methods: In vitro susceptibility to 27 antimicrobials was studied in twenty four Shigella flexneri strains isolated from stools. The presence of integrons class 1, 2 and 3 and antimicrobial resistance gene cassettes was investigated by polymerase chain reaction (PCR) using specific primers for each gene. Results: Most strains were resistant to one of the following antimicrobials: ampicillin, sulphonamide, trimethoprim, tetracycline, streptomycin, sulfamethoxazole-trimethoprim or chloramphenicol. Twenty nine percent were simultaneously resistant to all these antimicrobials. Integrons class 1 and 2 were found in 19 strains (79%). Class 3 integrons were not found. Gene cassettes dfrA1 and ant(3")I were associated to integrons class 2 in most strains (15/20, 75%). Genes cat, tetB and blarTEM were detected in 18/24 (75%), 7/24 (29%) and 4/24 (17%) of the strains, respectively and were not associated to any of the studied integrons. Genes that codify enzymes AAC(6')Ib and APH(3')VI were not detected in any strain. Conclusions: The high frequency of integrons found in the studied strains, could partly explain the increasing antimicrobial resistance of Shigella flexneri strains, isolated in Chile (Rev Méd Chile 2003; 131: 727-33)info:eu-repo/semantics/openAccessSociedad Médica de SantiagoRevista médica de Chile v.131 n.7 20032003-07-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872003000700003es10.4067/S0034-98872003000700003
institution	Scielo Chile
collection	Scielo Chile
language	Spanish / Castilian
topic	Drug resistance, microbial Gene library Integrons Shigella flexneri
spellingShingle	Drug resistance, microbial Gene library Integrons Shigella flexneri Muñoz A,Jeannette Bello T,Helia Domínguez Y,Mariana Mella M,Sergio Zemelman Z,Raúl González R,Gerardo Integrones y cassettes genéticos de resistencia a antimicrobianos en cepas de Shigella flexneri
description	Background: The resistance of Shigella flexneri to antimicrobial agents can be associated to the presence of integrons that may contain and express antimicrobial resistance gene cassettes. Aim: To study antimicrobial resistance and the presence of integrons and antimicrobial gene cassettes in Shigella flexneri strains. Material and methods: In vitro susceptibility to 27 antimicrobials was studied in twenty four Shigella flexneri strains isolated from stools. The presence of integrons class 1, 2 and 3 and antimicrobial resistance gene cassettes was investigated by polymerase chain reaction (PCR) using specific primers for each gene. Results: Most strains were resistant to one of the following antimicrobials: ampicillin, sulphonamide, trimethoprim, tetracycline, streptomycin, sulfamethoxazole-trimethoprim or chloramphenicol. Twenty nine percent were simultaneously resistant to all these antimicrobials. Integrons class 1 and 2 were found in 19 strains (79%). Class 3 integrons were not found. Gene cassettes dfrA1 and ant(3")I were associated to integrons class 2 in most strains (15/20, 75%). Genes cat, tetB and blarTEM were detected in 18/24 (75%), 7/24 (29%) and 4/24 (17%) of the strains, respectively and were not associated to any of the studied integrons. Genes that codify enzymes AAC(6')Ib and APH(3')VI were not detected in any strain. Conclusions: The high frequency of integrons found in the studied strains, could partly explain the increasing antimicrobial resistance of Shigella flexneri strains, isolated in Chile (Rev Méd Chile 2003; 131: 727-33)
author	Muñoz A,Jeannette Bello T,Helia Domínguez Y,Mariana Mella M,Sergio Zemelman Z,Raúl González R,Gerardo
author_facet	Muñoz A,Jeannette Bello T,Helia Domínguez Y,Mariana Mella M,Sergio Zemelman Z,Raúl González R,Gerardo
author_sort	Muñoz A,Jeannette
title	Integrones y cassettes genéticos de resistencia a antimicrobianos en cepas de Shigella flexneri
title_short	Integrones y cassettes genéticos de resistencia a antimicrobianos en cepas de Shigella flexneri
title_full	Integrones y cassettes genéticos de resistencia a antimicrobianos en cepas de Shigella flexneri
title_fullStr	Integrones y cassettes genéticos de resistencia a antimicrobianos en cepas de Shigella flexneri
title_full_unstemmed	Integrones y cassettes genéticos de resistencia a antimicrobianos en cepas de Shigella flexneri
title_sort	integrones y cassettes genéticos de resistencia a antimicrobianos en cepas de shigella flexneri
publisher	Sociedad Médica de Santiago
publishDate	2003
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872003000700003
work_keys_str_mv	AT munozajeannette integronesycassettesgeneticosderesistenciaaantimicrobianosencepasdeshigellaflexneri AT bellothelia integronesycassettesgeneticosderesistenciaaantimicrobianosencepasdeshigellaflexneri AT dominguezymariana integronesycassettesgeneticosderesistenciaaantimicrobianosencepasdeshigellaflexneri AT mellamsergio integronesycassettesgeneticosderesistenciaaantimicrobianosencepasdeshigellaflexneri AT zemelmanzraul integronesycassettesgeneticosderesistenciaaantimicrobianosencepasdeshigellaflexneri AT gonzalezrgerardo integronesycassettesgeneticosderesistenciaaantimicrobianosencepasdeshigellaflexneri
_version_	1718436100491444224

Integrones y cassettes genéticos de resistencia a antimicrobianos en cepas de Shigella flexneri

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