Tipificación del virus papiloma humano (VPH) en lesiones preneoplásicas y carcinoma del cuello uterino en mujeres de la IX Región-Chile

Background: Uterine cervical cancer (UCC) is an important public health problem in Chile. Although HPV infection has been established as the main cause of UCC, little is known of its frequency and distribution in our population. Aim: To determine the presence and frequency of viral genotypes in uter...

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Autores principales:	Melo A,Angélica, Montenegro H,Sonia, Hooper,Terryl, Capurro V,Italo, Roa S,Juan Carlos, Roa E,Iván
Lenguaje:	Spanish / Castilian
Publicado:	Sociedad Médica de Santiago 2003
Materias:	Cervix neoplasms Papillomavirus human
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872003001200004
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spelling	oai:scielo:S0034-988720030012000042004-04-16Tipificación del virus papiloma humano (VPH) en lesiones preneoplásicas y carcinoma del cuello uterino en mujeres de la IX Región-ChileMelo A,AngélicaMontenegro H,SoniaHooper,TerrylCapurro V,ItaloRoa S,Juan CarlosRoa E,Iván Cervix neoplasms Papillomavirus human Background: Uterine cervical cancer (UCC) is an important public health problem in Chile. Although HPV infection has been established as the main cause of UCC, little is known of its frequency and distribution in our population. Aim: To determine the presence and frequency of viral genotypes in uterine cervical specimens with preneoplastic and neoplastic lesions. Material and Methods: Two nested consensus PCRs followed by identification of amplified product by dot-blot hybridization and restriction fragment length polymorphism (RFLP) were used to analyze 175 biopsies. Results: Detection of HPV was 40% in cases without histological lesion, 88% in low grade lesions, 89% in high grade lesions (HGL) and 93.5% in invasive carcinoma. Of all HPV positive cases, 89.5% were classified as high risk and only a 4.9% of HPV cases were of low risk type. Six percent of cases had multiple infections. Distribution of viral genotypes according to RFLP was: HPV33 (25.3%), 16 (18.7%), 52 (13.3%), 31 (12%), 35 (6.6%), 18 (2.7%). Conclusions: Most HPV found in biopsies with HGL and UCC were of high risk genotype. The elevated presence of high risk HPV in patients without cervical lesions may be a factor that explains the high percentage of UCC cases in our region. Most common viral types were: HPV16, 31, 33 and 52. Viral detection and typing may provide valuable information for patient selection and follow up and for allocation of resources (Rev Méd Chile 2003; 131: 1382-90).info:eu-repo/semantics/openAccessSociedad Médica de SantiagoRevista médica de Chile v.131 n.12 20032003-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872003001200004es10.4067/S0034-98872003001200004
institution	Scielo Chile
collection	Scielo Chile
language	Spanish / Castilian
topic	Cervix neoplasms Papillomavirus human
spellingShingle	Cervix neoplasms Papillomavirus human Melo A,Angélica Montenegro H,Sonia Hooper,Terryl Capurro V,Italo Roa S,Juan Carlos Roa E,Iván Tipificación del virus papiloma humano (VPH) en lesiones preneoplásicas y carcinoma del cuello uterino en mujeres de la IX Región-Chile
description	Background: Uterine cervical cancer (UCC) is an important public health problem in Chile. Although HPV infection has been established as the main cause of UCC, little is known of its frequency and distribution in our population. Aim: To determine the presence and frequency of viral genotypes in uterine cervical specimens with preneoplastic and neoplastic lesions. Material and Methods: Two nested consensus PCRs followed by identification of amplified product by dot-blot hybridization and restriction fragment length polymorphism (RFLP) were used to analyze 175 biopsies. Results: Detection of HPV was 40% in cases without histological lesion, 88% in low grade lesions, 89% in high grade lesions (HGL) and 93.5% in invasive carcinoma. Of all HPV positive cases, 89.5% were classified as high risk and only a 4.9% of HPV cases were of low risk type. Six percent of cases had multiple infections. Distribution of viral genotypes according to RFLP was: HPV33 (25.3%), 16 (18.7%), 52 (13.3%), 31 (12%), 35 (6.6%), 18 (2.7%). Conclusions: Most HPV found in biopsies with HGL and UCC were of high risk genotype. The elevated presence of high risk HPV in patients without cervical lesions may be a factor that explains the high percentage of UCC cases in our region. Most common viral types were: HPV16, 31, 33 and 52. Viral detection and typing may provide valuable information for patient selection and follow up and for allocation of resources (Rev Méd Chile 2003; 131: 1382-90).
author	Melo A,Angélica Montenegro H,Sonia Hooper,Terryl Capurro V,Italo Roa S,Juan Carlos Roa E,Iván
author_facet	Melo A,Angélica Montenegro H,Sonia Hooper,Terryl Capurro V,Italo Roa S,Juan Carlos Roa E,Iván
author_sort	Melo A,Angélica
title	Tipificación del virus papiloma humano (VPH) en lesiones preneoplásicas y carcinoma del cuello uterino en mujeres de la IX Región-Chile
title_short	Tipificación del virus papiloma humano (VPH) en lesiones preneoplásicas y carcinoma del cuello uterino en mujeres de la IX Región-Chile
title_full	Tipificación del virus papiloma humano (VPH) en lesiones preneoplásicas y carcinoma del cuello uterino en mujeres de la IX Región-Chile
title_fullStr	Tipificación del virus papiloma humano (VPH) en lesiones preneoplásicas y carcinoma del cuello uterino en mujeres de la IX Región-Chile
title_full_unstemmed	Tipificación del virus papiloma humano (VPH) en lesiones preneoplásicas y carcinoma del cuello uterino en mujeres de la IX Región-Chile
title_sort	tipificación del virus papiloma humano (vph) en lesiones preneoplásicas y carcinoma del cuello uterino en mujeres de la ix región-chile
publisher	Sociedad Médica de Santiago
publishDate	2003
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872003001200004
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Tipificación del virus papiloma humano (VPH) en lesiones preneoplásicas y carcinoma del cuello uterino en mujeres de la IX Región-Chile

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