Mutación del gen K-ras en el cáncer de la vesícula biliar

Background: Different K-ras mutation frequencies in gallbladder cancer have been reported. Aim: To study the frequency of K-ras gene mutations in advanced gallbladder carcinoma not associated to anomalous junction of pancreatic-biliary duct (AJPBD). Material and methods: 33 formalin fixed paraffin e...

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Autores principales:	Roa S,Juan Carlos, Roa E,Iván, Aretxabala U,Xabier de, Melo A,Angélica, Faría O,Gaspar, Tapia E,Oscar
Lenguaje:	Spanish / Castilian
Publicado:	Sociedad Médica de Santiago 2004
Materias:	Gallbladder neoplasms Genes, ra K-ras gene
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872004000800007
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id	oai:scielo:S0034-98872004000800007
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spelling	oai:scielo:S0034-988720040008000072014-08-14Mutación del gen K-ras en el cáncer de la vesícula biliarRoa S,Juan CarlosRoa E,IvánAretxabala U,Xabier deMelo A,AngélicaFaría O,GasparTapia E,Oscar Gallbladder neoplasms Genes, ra K-ras gene Background: Different K-ras mutation frequencies in gallbladder cancer have been reported. Aim: To study the frequency of K-ras gene mutations in advanced gallbladder carcinoma not associated to anomalous junction of pancreatic-biliary duct (AJPBD). Material and methods: 33 formalin fixed paraffin embedded samples of gallbladder carcinoma (30 women, age range 32-86 years) were selected. Pancreatic cancer tissue with K-ras mutations was used as control. DNA was extracted from the histological section by mean of microdissection and K-ras mutations in codon 12 were detected by polymerase chain reaction and restriction fragment length polymorphism (RFLP), using previously reported technique. Results: Most cases were poorly differentiated adenocarcinomas. K-ras mutation was detected in 10 cases (30%) samples. No differences in K-ras mutation frequency between subserous and serous tumors were detected and no relation between histological features and the mutation was observed. Conclusions: K-ras mutation in codon 12 is present in 30% in our advanced gallbladder carcinomas. The study of K-ras mutation in preneoplastic lesions and early carcinomas will be important to determine the role of this gene in the gallbladder carcinogenesis in Chile (Rev Méd Chile 2004; 132: 955-60)info:eu-repo/semantics/openAccessSociedad Médica de SantiagoRevista médica de Chile v.132 n.8 20042004-08-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872004000800007es10.4067/S0034-98872004000800007
institution	Scielo Chile
collection	Scielo Chile
language	Spanish / Castilian
topic	Gallbladder neoplasms Genes, ra K-ras gene
spellingShingle	Gallbladder neoplasms Genes, ra K-ras gene Roa S,Juan Carlos Roa E,Iván Aretxabala U,Xabier de Melo A,Angélica Faría O,Gaspar Tapia E,Oscar Mutación del gen K-ras en el cáncer de la vesícula biliar
description	Background: Different K-ras mutation frequencies in gallbladder cancer have been reported. Aim: To study the frequency of K-ras gene mutations in advanced gallbladder carcinoma not associated to anomalous junction of pancreatic-biliary duct (AJPBD). Material and methods: 33 formalin fixed paraffin embedded samples of gallbladder carcinoma (30 women, age range 32-86 years) were selected. Pancreatic cancer tissue with K-ras mutations was used as control. DNA was extracted from the histological section by mean of microdissection and K-ras mutations in codon 12 were detected by polymerase chain reaction and restriction fragment length polymorphism (RFLP), using previously reported technique. Results: Most cases were poorly differentiated adenocarcinomas. K-ras mutation was detected in 10 cases (30%) samples. No differences in K-ras mutation frequency between subserous and serous tumors were detected and no relation between histological features and the mutation was observed. Conclusions: K-ras mutation in codon 12 is present in 30% in our advanced gallbladder carcinomas. The study of K-ras mutation in preneoplastic lesions and early carcinomas will be important to determine the role of this gene in the gallbladder carcinogenesis in Chile (Rev Méd Chile 2004; 132: 955-60)
author	Roa S,Juan Carlos Roa E,Iván Aretxabala U,Xabier de Melo A,Angélica Faría O,Gaspar Tapia E,Oscar
author_facet	Roa S,Juan Carlos Roa E,Iván Aretxabala U,Xabier de Melo A,Angélica Faría O,Gaspar Tapia E,Oscar
author_sort	Roa S,Juan Carlos
title	Mutación del gen K-ras en el cáncer de la vesícula biliar
title_short	Mutación del gen K-ras en el cáncer de la vesícula biliar
title_full	Mutación del gen K-ras en el cáncer de la vesícula biliar
title_fullStr	Mutación del gen K-ras en el cáncer de la vesícula biliar
title_full_unstemmed	Mutación del gen K-ras en el cáncer de la vesícula biliar
title_sort	mutación del gen k-ras en el cáncer de la vesícula biliar
publisher	Sociedad Médica de Santiago
publishDate	2004
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872004000800007
work_keys_str_mv	AT roasjuancarlos mutaciondelgenkrasenelcancerdelavesiculabiliar AT roaeivan mutaciondelgenkrasenelcancerdelavesiculabiliar AT aretxabalauxabierde mutaciondelgenkrasenelcancerdelavesiculabiliar AT meloaangelica mutaciondelgenkrasenelcancerdelavesiculabiliar AT fariaogaspar mutaciondelgenkrasenelcancerdelavesiculabiliar AT tapiaeoscar mutaciondelgenkrasenelcancerdelavesiculabiliar
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Mutación del gen K-ras en el cáncer de la vesícula biliar

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