P450 arom y microambiente estrogénico en endometrios eutópicos de mujeres con endometriosis

Background: Endometriosis, a common gynecologic disorder characterized by endometrial glands and stroma outside the uterus, is diagnosed by direct visualization of peritoneal and ovarian implants during laparoscopy. Aim: To study the estrogenic microenvironment in eutopic endometria of women with an...

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Autores principales:	Johnson P,M Cecilia, Pinto O,Claudio, Alves L,Alessandra, Palomino A,Alberto, Fuentes G,Ariel, Boric S,M Angélica, Vega B,Margarita
Lenguaje:	Spanish / Castilian
Publicado:	Sociedad Médica de Santiago 2004
Materias:	Aromatase Cytochrome P450 Endometriosis Genital neoplasms female: P450 AROM
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872004001200004
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spelling	oai:scielo:S0034-988720040012000042006-03-21P450 arom y microambiente estrogénico en endometrios eutópicos de mujeres con endometriosisJohnson P,M CeciliaPinto O,ClaudioAlves L,AlessandraPalomino A,AlbertoFuentes G,ArielBoric S,M AngélicaVega B,Margarita Aromatase Cytochrome P450 Endometriosis Genital neoplasms female: P450 AROM Background: Endometriosis, a common gynecologic disorder characterized by endometrial glands and stroma outside the uterus, is diagnosed by direct visualization of peritoneal and ovarian implants during laparoscopy. Aim: To study the estrogenic microenvironment in eutopic endometria of women with and without endometriosis. Patients and methods: Eutopic endometria, obtained during laparoscopy from 23 women with endometriosis and 20 fertile cyclic women undergoing tubal sterilization, was studied. P450Arom mRNA expression (RT-PCR) was measured. Also, P450Arom activity was assessed measuring testosterone conversion to estradiol and the concentration of this last hormone in cultured endometrial explants. Results: Age and body mass index was similar in both groups studied. Seventy nine percent of endometria from women with endometriosis and in 29.4% from control group expressed P450Arom mRNA (p <0.01). The intensity of the band was higher in secretory endometria from women with endometriosis when compared to controls (p <0.01), but it was similar during the proliferative phase. Estradiol secretion to the culture media by proliferative endometria explants from women with endometriosis was 3-fold higher than secretory endometria (p <0.01) and endometria from control women in both phases. P450Arom activity, in the presence of testosterone, was 7-fold higher in endometrial cultures from women with endometriosis, when compare with the basal culture (p <0.01). However, in endometrial explant cultures from control women, this activity was not statistical different. Conclusions: These results indicate that in women with endometriosis, the microenvironment in the endometria is estrogenic as a consequence of an increased expression and activity of the P450 Arom (Rev Méd Chile 2004; 132: 1475-82).info:eu-repo/semantics/openAccessSociedad Médica de SantiagoRevista médica de Chile v.132 n.12 20042004-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872004001200004es10.4067/S0034-98872004001200004
institution	Scielo Chile
collection	Scielo Chile
language	Spanish / Castilian
topic	Aromatase Cytochrome P450 Endometriosis Genital neoplasms female: P450 AROM
spellingShingle	Aromatase Cytochrome P450 Endometriosis Genital neoplasms female: P450 AROM Johnson P,M Cecilia Pinto O,Claudio Alves L,Alessandra Palomino A,Alberto Fuentes G,Ariel Boric S,M Angélica Vega B,Margarita P450 arom y microambiente estrogénico en endometrios eutópicos de mujeres con endometriosis
description	Background: Endometriosis, a common gynecologic disorder characterized by endometrial glands and stroma outside the uterus, is diagnosed by direct visualization of peritoneal and ovarian implants during laparoscopy. Aim: To study the estrogenic microenvironment in eutopic endometria of women with and without endometriosis. Patients and methods: Eutopic endometria, obtained during laparoscopy from 23 women with endometriosis and 20 fertile cyclic women undergoing tubal sterilization, was studied. P450Arom mRNA expression (RT-PCR) was measured. Also, P450Arom activity was assessed measuring testosterone conversion to estradiol and the concentration of this last hormone in cultured endometrial explants. Results: Age and body mass index was similar in both groups studied. Seventy nine percent of endometria from women with endometriosis and in 29.4% from control group expressed P450Arom mRNA (p <0.01). The intensity of the band was higher in secretory endometria from women with endometriosis when compared to controls (p <0.01), but it was similar during the proliferative phase. Estradiol secretion to the culture media by proliferative endometria explants from women with endometriosis was 3-fold higher than secretory endometria (p <0.01) and endometria from control women in both phases. P450Arom activity, in the presence of testosterone, was 7-fold higher in endometrial cultures from women with endometriosis, when compare with the basal culture (p <0.01). However, in endometrial explant cultures from control women, this activity was not statistical different. Conclusions: These results indicate that in women with endometriosis, the microenvironment in the endometria is estrogenic as a consequence of an increased expression and activity of the P450 Arom (Rev Méd Chile 2004; 132: 1475-82).
author	Johnson P,M Cecilia Pinto O,Claudio Alves L,Alessandra Palomino A,Alberto Fuentes G,Ariel Boric S,M Angélica Vega B,Margarita
author_facet	Johnson P,M Cecilia Pinto O,Claudio Alves L,Alessandra Palomino A,Alberto Fuentes G,Ariel Boric S,M Angélica Vega B,Margarita
author_sort	Johnson P,M Cecilia
title	P450 arom y microambiente estrogénico en endometrios eutópicos de mujeres con endometriosis
title_short	P450 arom y microambiente estrogénico en endometrios eutópicos de mujeres con endometriosis
title_full	P450 arom y microambiente estrogénico en endometrios eutópicos de mujeres con endometriosis
title_fullStr	P450 arom y microambiente estrogénico en endometrios eutópicos de mujeres con endometriosis
title_full_unstemmed	P450 arom y microambiente estrogénico en endometrios eutópicos de mujeres con endometriosis
title_sort	p450 arom y microambiente estrogénico en endometrios eutópicos de mujeres con endometriosis
publisher	Sociedad Médica de Santiago
publishDate	2004
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872004001200004
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P450 arom y microambiente estrogénico en endometrios eutópicos de mujeres con endometriosis

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