Lack of mutation in exon 10 of p53 gene in thyroid tumors

Background: p53 is a nuclear protein that exerts an important role in the negative control of cellular proliferation, as well as in masterminding signaling cascades important in DNA repair and/or apoptosis. Mutations of p53 have been reported with high frequency in many cancer types and are highly p...

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Autores principales:	Lia Santarosa,Patricia, Granja,Fabiana, Cristina Morari,Elaine, Leite,Janaína Luisa, Vera Montalli da Assumpção,Ligia, Ward,Laura S
Lenguaje:	English
Publicado:	Sociedad Médica de Santiago 2004
Materias:	Genes, p53 Mutation Thyroid gland Tumor protein p53-binding protein
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872004001200009
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spelling	oai:scielo:S0034-988720040012000092006-03-21Lack of mutation in exon 10 of p53 gene in thyroid tumorsLia Santarosa,PatriciaGranja,FabianaCristina Morari,ElaineLeite,Janaína LuisaVera Montalli da Assumpção,LigiaWard,Laura S Genes, p53 Mutation Thyroid gland Tumor protein p53-binding protein Background: p53 is a nuclear protein that exerts an important role in the negative control of cellular proliferation, as well as in masterminding signaling cascades important in DNA repair and/or apoptosis. Mutations of p53 have been reported with high frequency in many cancer types and are highly prevalent in poorly differentiated and undifferentiated thyroid carcinomas, but they are not found in benign tumors and are infrequent in well-differentiated cancer. Most mutations are located in exons 5-8 of the gene. Recently, a germline mutation in the seldom investigated exon 10, on codon 337 of p53 was described in Brazilian children who had adrenocortical tumors. Aim: To study codon 337 of exon 10 of p53 mutation in thyroid tumors. Material and methods: Seventy four thyroid tumors were studied (5 follicular carcinomas including 3 widely invasive, 22 papillary carcinomas including 6 tall cell variants, 11 follicular adenomas, 1 medullary carcinoma and 35 benign goiters). DNA was extracted from a central part of all tumors and contralateral normal thyroid tissue samples or blood from 38 of these patients. The products of PCR for exon 10 of p53 were examined by single strand conformation polymorphism (SSCP) analysis. We sequenced 2 samples suspected of presenting aberrant migrating bands and 3 additional PCR products from tumor samples with normal SSCP patterns but all were wild type. Results: In all samples studied, a wild type sequence was found. Conclusions: Exon 10 of p53 gene does not present mutations in thyroid tumors, suggesting that this mutation is specific of adrenocortical cancers. (Rev Méd Chile 2004; 132: 1513-6)info:eu-repo/semantics/openAccessSociedad Médica de SantiagoRevista médica de Chile v.132 n.12 20042004-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872004001200009en10.4067/S0034-98872004001200009
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Genes, p53 Mutation Thyroid gland Tumor protein p53-binding protein
spellingShingle	Genes, p53 Mutation Thyroid gland Tumor protein p53-binding protein Lia Santarosa,Patricia Granja,Fabiana Cristina Morari,Elaine Leite,Janaína Luisa Vera Montalli da Assumpção,Ligia Ward,Laura S Lack of mutation in exon 10 of p53 gene in thyroid tumors
description	Background: p53 is a nuclear protein that exerts an important role in the negative control of cellular proliferation, as well as in masterminding signaling cascades important in DNA repair and/or apoptosis. Mutations of p53 have been reported with high frequency in many cancer types and are highly prevalent in poorly differentiated and undifferentiated thyroid carcinomas, but they are not found in benign tumors and are infrequent in well-differentiated cancer. Most mutations are located in exons 5-8 of the gene. Recently, a germline mutation in the seldom investigated exon 10, on codon 337 of p53 was described in Brazilian children who had adrenocortical tumors. Aim: To study codon 337 of exon 10 of p53 mutation in thyroid tumors. Material and methods: Seventy four thyroid tumors were studied (5 follicular carcinomas including 3 widely invasive, 22 papillary carcinomas including 6 tall cell variants, 11 follicular adenomas, 1 medullary carcinoma and 35 benign goiters). DNA was extracted from a central part of all tumors and contralateral normal thyroid tissue samples or blood from 38 of these patients. The products of PCR for exon 10 of p53 were examined by single strand conformation polymorphism (SSCP) analysis. We sequenced 2 samples suspected of presenting aberrant migrating bands and 3 additional PCR products from tumor samples with normal SSCP patterns but all were wild type. Results: In all samples studied, a wild type sequence was found. Conclusions: Exon 10 of p53 gene does not present mutations in thyroid tumors, suggesting that this mutation is specific of adrenocortical cancers. (Rev Méd Chile 2004; 132: 1513-6)
author	Lia Santarosa,Patricia Granja,Fabiana Cristina Morari,Elaine Leite,Janaína Luisa Vera Montalli da Assumpção,Ligia Ward,Laura S
author_facet	Lia Santarosa,Patricia Granja,Fabiana Cristina Morari,Elaine Leite,Janaína Luisa Vera Montalli da Assumpção,Ligia Ward,Laura S
author_sort	Lia Santarosa,Patricia
title	Lack of mutation in exon 10 of p53 gene in thyroid tumors
title_short	Lack of mutation in exon 10 of p53 gene in thyroid tumors
title_full	Lack of mutation in exon 10 of p53 gene in thyroid tumors
title_fullStr	Lack of mutation in exon 10 of p53 gene in thyroid tumors
title_full_unstemmed	Lack of mutation in exon 10 of p53 gene in thyroid tumors
title_sort	lack of mutation in exon 10 of p53 gene in thyroid tumors
publisher	Sociedad Médica de Santiago
publishDate	2004
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872004001200009
work_keys_str_mv	AT liasantarosapatricia lackofmutationinexon10ofp53geneinthyroidtumors AT granjafabiana lackofmutationinexon10ofp53geneinthyroidtumors AT cristinamorarielaine lackofmutationinexon10ofp53geneinthyroidtumors AT leitejanainaluisa lackofmutationinexon10ofp53geneinthyroidtumors AT veramontallidaassumpcaoligia lackofmutationinexon10ofp53geneinthyroidtumors AT wardlauras lackofmutationinexon10ofp53geneinthyroidtumors
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Lack of mutation in exon 10 of p53 gene in thyroid tumors

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