Estudio del patrón de metilación génico en el cáncer gástrico en Chile

Background:Promoter genomic DNA methylation is an important inactivation mechanism of tumor suppressor genes. This genetic-molecular pathway for cancer may separate a subset of patients with different prognoses and eventually different responses to specific therapies. Aim: To analyze the methylation...

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Autores principales:	Roa S,Juan Carlos, Anabalón R,Leonardo, Roa E,Iván, Tapia E,Oscar, Melo,Angélica, Villaseca H,Miguel, Araya O,Juan Carlos
Lenguaje:	Spanish / Castilian
Publicado:	Sociedad Médica de Santiago 2005
Materias:	DNA methylation Promoter regions (Genetics) Stomach neoplasms
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872005000800002
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spelling	oai:scielo:S0034-988720050008000022014-08-12Estudio del patrón de metilación génico en el cáncer gástrico en ChileRoa S,Juan CarlosAnabalón R,LeonardoRoa E,IvánTapia E,OscarMelo,AngélicaVillaseca H,MiguelAraya O,Juan Carlos DNA methylation Promoter regions (Genetics) Stomach neoplasms Background:Promoter genomic DNA methylation is an important inactivation mechanism of tumor suppressor genes. This genetic-molecular pathway for cancer may separate a subset of patients with different prognoses and eventually different responses to specific therapies. Aim: To analyze the methylation pattern of important genes related to different carcinogenic mechanisms in patients with gastric cancer (GC) and the relationship with its morphological features and biological behavior. Material and methods: Forty-seven fresh-frozen GC samples were selected. The methylation-specific PCR (MSP) test was used to analyze promoter methylation status for genes MLH1, CDKN2A (p16), APC, CDH1 (Cadherin E) and FHIT. Follow-up and complete morphological features were obtained for all cases. Results: We found methylation in at least one of the genes studied in 83% of the cases. The frequencies of promoter hypermethylation of MLH1, CDKN2A, APC, CDH1 and FHIT were 31%, 43%, 46%, 80% y 62%, respectively. We found a relationship between APC methylation and good histological differentiation (p=0.03); CDH1 methylation with diffuse type by Lauren and 3 or more metastasic lymph nodes (p <0.05); FHIT, CDKN2A and CDH1 methylation and female condition (p <0.04). We also found a non-significant relationship between CDKN2A methylation and better survival (p=0.07). Conclusions: The high frequency promoter methylation found confirms its importance in gastric carcinogenesis. The finding of alterations in the methylation pattern of genes studied and its association with prognostic factors is a helpful tool in the search for new criteria in clinical and therapeutic decision makinginfo:eu-repo/semantics/openAccessSociedad Médica de SantiagoRevista médica de Chile v.133 n.8 20052005-08-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872005000800002es10.4067/S0034-98872005000800002
institution	Scielo Chile
collection	Scielo Chile
language	Spanish / Castilian
topic	DNA methylation Promoter regions (Genetics) Stomach neoplasms
spellingShingle	DNA methylation Promoter regions (Genetics) Stomach neoplasms Roa S,Juan Carlos Anabalón R,Leonardo Roa E,Iván Tapia E,Oscar Melo,Angélica Villaseca H,Miguel Araya O,Juan Carlos Estudio del patrón de metilación génico en el cáncer gástrico en Chile
description	Background:Promoter genomic DNA methylation is an important inactivation mechanism of tumor suppressor genes. This genetic-molecular pathway for cancer may separate a subset of patients with different prognoses and eventually different responses to specific therapies. Aim: To analyze the methylation pattern of important genes related to different carcinogenic mechanisms in patients with gastric cancer (GC) and the relationship with its morphological features and biological behavior. Material and methods: Forty-seven fresh-frozen GC samples were selected. The methylation-specific PCR (MSP) test was used to analyze promoter methylation status for genes MLH1, CDKN2A (p16), APC, CDH1 (Cadherin E) and FHIT. Follow-up and complete morphological features were obtained for all cases. Results: We found methylation in at least one of the genes studied in 83% of the cases. The frequencies of promoter hypermethylation of MLH1, CDKN2A, APC, CDH1 and FHIT were 31%, 43%, 46%, 80% y 62%, respectively. We found a relationship between APC methylation and good histological differentiation (p=0.03); CDH1 methylation with diffuse type by Lauren and 3 or more metastasic lymph nodes (p <0.05); FHIT, CDKN2A and CDH1 methylation and female condition (p <0.04). We also found a non-significant relationship between CDKN2A methylation and better survival (p=0.07). Conclusions: The high frequency promoter methylation found confirms its importance in gastric carcinogenesis. The finding of alterations in the methylation pattern of genes studied and its association with prognostic factors is a helpful tool in the search for new criteria in clinical and therapeutic decision making
author	Roa S,Juan Carlos Anabalón R,Leonardo Roa E,Iván Tapia E,Oscar Melo,Angélica Villaseca H,Miguel Araya O,Juan Carlos
author_facet	Roa S,Juan Carlos Anabalón R,Leonardo Roa E,Iván Tapia E,Oscar Melo,Angélica Villaseca H,Miguel Araya O,Juan Carlos
author_sort	Roa S,Juan Carlos
title	Estudio del patrón de metilación génico en el cáncer gástrico en Chile
title_short	Estudio del patrón de metilación génico en el cáncer gástrico en Chile
title_full	Estudio del patrón de metilación génico en el cáncer gástrico en Chile
title_fullStr	Estudio del patrón de metilación génico en el cáncer gástrico en Chile
title_full_unstemmed	Estudio del patrón de metilación génico en el cáncer gástrico en Chile
title_sort	estudio del patrón de metilación génico en el cáncer gástrico en chile
publisher	Sociedad Médica de Santiago
publishDate	2005
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872005000800002
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Estudio del patrón de metilación génico en el cáncer gástrico en Chile

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