Asociación de la región microsatélite AGAT del gen receptor de mineralocorticoides con los niveles de actividad renina en hipertensos esenciales

Background: Hypertensive states could result from constitutive activation of mineralorticoid receptor (MR) that generates salt retention and blood pressure elevation. Moreover, microsatellite regions can be associated to the regulation of the gene expression, producing subtle pathologies. Aim: To de...

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Autores principales:	Muñoz-Brauning,Carlos R, Carvajal,Cristián A, Mosso,Lorena M, Valdivia,Gonzalo, Fardella,Carlos E
Lenguaje:	Spanish / Castilian
Publicado:	Sociedad Médica de Santiago 2005
Materias:	Aldosterone Hypertension Receptors mineralocortidoid Renin
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872005001200002
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spelling	oai:scielo:S0034-988720050012000022006-01-27Asociación de la región microsatélite AGAT del gen receptor de mineralocorticoides con los niveles de actividad renina en hipertensos esencialesMuñoz-Brauning,Carlos RCarvajal,Cristián AMosso,Lorena MValdivia,GonzaloFardella,Carlos E Aldosterone Hypertension Receptors mineralocortidoid Renin Background: Hypertensive states could result from constitutive activation of mineralorticoid receptor (MR) that generates salt retention and blood pressure elevation. Moreover, microsatellite regions can be associated to the regulation of the gene expression, producing subtle pathologies. Aim: To determine the influence of microsatellite marker AGAT of the mineralocorticoid receptor gene in the plasma renin activity (PRA) and serum aldosterone (SA) levels of essential hypertensives (HT). Patients and Methods: We studied 292 HT patients and 57 normotensive (NT) controls. Blood samples were collected for PRA, SA and DNA isolation. Subjects were genotyped according to the length of the tetranucleotide AGAT repeat using polymerase chain reaction and polyacrylamide gel electrophoresis. Based on the normal distribution, we considered 13 to 15 repeats as a habitual (H) length and less than 13 or more than 15 repeats, as non-habitual (non-H). Results: We detected 8 different lengths in the AGAT repeat (allele) in both groups, ranging from 9-17 repeats, where the allele 11 was not detected in either hypertensive or normotensive groups. The allelic distribution was different in both groups (c²=37.57, 4GL, p <0.001). In hypertensive patients, the H group showed higher PRA levels (median (Q1-Q3)) than the non-H group: 1.3 (0-7-3.5) vs 1.0 (0.5-2.3) ng/mLh, p <0.05. The SA levels did not show differences between both groups, but the SAPRA product was higher in the H group than the no-H group: 9.3 (3.0-24.6) vs 6.5 (2.5-14.6) p <0.05. In normotensive patients, no differences were observed in PRA, SA and SA*PRA between both groups. Conclusion: These results show association between the length of the AGAT repeat with the PRA in HT, suggesting a plausible role in the control of the MR gene expression, and secondarily in the regulation of blood pressure (Rev Méd Chile 2005; 133: 1415-23)info:eu-repo/semantics/openAccessSociedad Médica de SantiagoRevista médica de Chile v.133 n.12 20052005-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872005001200002es10.4067/S0034-98872005001200002
institution	Scielo Chile
collection	Scielo Chile
language	Spanish / Castilian
topic	Aldosterone Hypertension Receptors mineralocortidoid Renin
spellingShingle	Aldosterone Hypertension Receptors mineralocortidoid Renin Muñoz-Brauning,Carlos R Carvajal,Cristián A Mosso,Lorena M Valdivia,Gonzalo Fardella,Carlos E Asociación de la región microsatélite AGAT del gen receptor de mineralocorticoides con los niveles de actividad renina en hipertensos esenciales
description	Background: Hypertensive states could result from constitutive activation of mineralorticoid receptor (MR) that generates salt retention and blood pressure elevation. Moreover, microsatellite regions can be associated to the regulation of the gene expression, producing subtle pathologies. Aim: To determine the influence of microsatellite marker AGAT of the mineralocorticoid receptor gene in the plasma renin activity (PRA) and serum aldosterone (SA) levels of essential hypertensives (HT). Patients and Methods: We studied 292 HT patients and 57 normotensive (NT) controls. Blood samples were collected for PRA, SA and DNA isolation. Subjects were genotyped according to the length of the tetranucleotide AGAT repeat using polymerase chain reaction and polyacrylamide gel electrophoresis. Based on the normal distribution, we considered 13 to 15 repeats as a habitual (H) length and less than 13 or more than 15 repeats, as non-habitual (non-H). Results: We detected 8 different lengths in the AGAT repeat (allele) in both groups, ranging from 9-17 repeats, where the allele 11 was not detected in either hypertensive or normotensive groups. The allelic distribution was different in both groups (c²=37.57, 4GL, p <0.001). In hypertensive patients, the H group showed higher PRA levels (median (Q1-Q3)) than the non-H group: 1.3 (0-7-3.5) vs 1.0 (0.5-2.3) ng/mLh, p <0.05. The SA levels did not show differences between both groups, but the SAPRA product was higher in the H group than the no-H group: 9.3 (3.0-24.6) vs 6.5 (2.5-14.6) p <0.05. In normotensive patients, no differences were observed in PRA, SA and SA*PRA between both groups. Conclusion: These results show association between the length of the AGAT repeat with the PRA in HT, suggesting a plausible role in the control of the MR gene expression, and secondarily in the regulation of blood pressure (Rev Méd Chile 2005; 133: 1415-23)
author	Muñoz-Brauning,Carlos R Carvajal,Cristián A Mosso,Lorena M Valdivia,Gonzalo Fardella,Carlos E
author_facet	Muñoz-Brauning,Carlos R Carvajal,Cristián A Mosso,Lorena M Valdivia,Gonzalo Fardella,Carlos E
author_sort	Muñoz-Brauning,Carlos R
title	Asociación de la región microsatélite AGAT del gen receptor de mineralocorticoides con los niveles de actividad renina en hipertensos esenciales
title_short	Asociación de la región microsatélite AGAT del gen receptor de mineralocorticoides con los niveles de actividad renina en hipertensos esenciales
title_full	Asociación de la región microsatélite AGAT del gen receptor de mineralocorticoides con los niveles de actividad renina en hipertensos esenciales
title_fullStr	Asociación de la región microsatélite AGAT del gen receptor de mineralocorticoides con los niveles de actividad renina en hipertensos esenciales
title_full_unstemmed	Asociación de la región microsatélite AGAT del gen receptor de mineralocorticoides con los niveles de actividad renina en hipertensos esenciales
title_sort	asociación de la región microsatélite agat del gen receptor de mineralocorticoides con los niveles de actividad renina en hipertensos esenciales
publisher	Sociedad Médica de Santiago
publishDate	2005
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872005001200002
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Asociación de la región microsatélite AGAT del gen receptor de mineralocorticoides con los niveles de actividad renina en hipertensos esenciales

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