Mutación del gen K-ras en tumores pancreático-biliares

Background: The ras gene family (H-ras, N-ras and K-ras) are oncogenes that mutate frequently in human cancer, specially in tumors of the biliary tract and pancreas. Aim: To determine the frequency of K-ras gene codon 12 mutation in pancreatic and biliary tumors. Material and Methods: Samples of 35...

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Autores principales:	Roa S,Juan Carlos, Anabalón R,Leonardo, Tapia E,Oscar, Melo A,Angélica, de Aretxabala U,Xabier, Roa E,Iván
Lenguaje:	Spanish / Castilian
Publicado:	Sociedad Médica de Santiago 2005
Materias:	Biliary tract neoplasms Gallbladder neoplasms Genes ras Pancreatic neoplasms
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872005001200004
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spelling	oai:scielo:S0034-988720050012000042006-01-27Mutación del gen K-ras en tumores pancreático-biliaresRoa S,Juan CarlosAnabalón R,LeonardoTapia E,OscarMelo A,Angélicade Aretxabala U,XabierRoa E,Iván Biliary tract neoplasms Gallbladder neoplasms Genes ras Pancreatic neoplasms Background: The ras gene family (H-ras, N-ras and K-ras) are oncogenes that mutate frequently in human cancer, specially in tumors of the biliary tract and pancreas. Aim: To determine the frequency of K-ras gene codon 12 mutation in pancreatic and biliary tumors. Material and Methods: Samples of 35 gallbladder, 15 ampulla of Vater, 10 biliary tract and 9 pancreatic tumors, were analyzed. The tumor tissue was microdissected from paraffin embedded biopsies. The mutation was detected by a combination of polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP). Results: Overall, 46% of samples had K-ras gene mutations. Mutation frequency was 80, 56, 50 and 29% for ampulla of Vater, pancreatic, biliary tract and gallbladder tumors, respectively. When compared with the rest, gallbladder tumors had a significantly lower frequency of the mutation. Median survival for biliary tract tumors was 6 months, compared with 65 months for gallbladder tumors (p <0.05). Conclusions: Gallbladder carcinoma had the lower frequency of K-ras mutation, when compared with pancreatic, biliary tract and ampulla of Vater tumors (Rev Méd Chile 2005; 133: 1434-40)info:eu-repo/semantics/openAccessSociedad Médica de SantiagoRevista médica de Chile v.133 n.12 20052005-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872005001200004es10.4067/S0034-98872005001200004
institution	Scielo Chile
collection	Scielo Chile
language	Spanish / Castilian
topic	Biliary tract neoplasms Gallbladder neoplasms Genes ras Pancreatic neoplasms
spellingShingle	Biliary tract neoplasms Gallbladder neoplasms Genes ras Pancreatic neoplasms Roa S,Juan Carlos Anabalón R,Leonardo Tapia E,Oscar Melo A,Angélica de Aretxabala U,Xabier Roa E,Iván Mutación del gen K-ras en tumores pancreático-biliares
description	Background: The ras gene family (H-ras, N-ras and K-ras) are oncogenes that mutate frequently in human cancer, specially in tumors of the biliary tract and pancreas. Aim: To determine the frequency of K-ras gene codon 12 mutation in pancreatic and biliary tumors. Material and Methods: Samples of 35 gallbladder, 15 ampulla of Vater, 10 biliary tract and 9 pancreatic tumors, were analyzed. The tumor tissue was microdissected from paraffin embedded biopsies. The mutation was detected by a combination of polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP). Results: Overall, 46% of samples had K-ras gene mutations. Mutation frequency was 80, 56, 50 and 29% for ampulla of Vater, pancreatic, biliary tract and gallbladder tumors, respectively. When compared with the rest, gallbladder tumors had a significantly lower frequency of the mutation. Median survival for biliary tract tumors was 6 months, compared with 65 months for gallbladder tumors (p <0.05). Conclusions: Gallbladder carcinoma had the lower frequency of K-ras mutation, when compared with pancreatic, biliary tract and ampulla of Vater tumors (Rev Méd Chile 2005; 133: 1434-40)
author	Roa S,Juan Carlos Anabalón R,Leonardo Tapia E,Oscar Melo A,Angélica de Aretxabala U,Xabier Roa E,Iván
author_facet	Roa S,Juan Carlos Anabalón R,Leonardo Tapia E,Oscar Melo A,Angélica de Aretxabala U,Xabier Roa E,Iván
author_sort	Roa S,Juan Carlos
title	Mutación del gen K-ras en tumores pancreático-biliares
title_short	Mutación del gen K-ras en tumores pancreático-biliares
title_full	Mutación del gen K-ras en tumores pancreático-biliares
title_fullStr	Mutación del gen K-ras en tumores pancreático-biliares
title_full_unstemmed	Mutación del gen K-ras en tumores pancreático-biliares
title_sort	mutación del gen k-ras en tumores pancreático-biliares
publisher	Sociedad Médica de Santiago
publishDate	2005
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872005001200004
work_keys_str_mv	AT roasjuancarlos mutaciondelgenkrasentumorespancreaticobiliares AT anabalonrleonardo mutaciondelgenkrasentumorespancreaticobiliares AT tapiaeoscar mutaciondelgenkrasentumorespancreaticobiliares AT meloaangelica mutaciondelgenkrasentumorespancreaticobiliares AT dearetxabalauxabier mutaciondelgenkrasentumorespancreaticobiliares AT roaeivan mutaciondelgenkrasentumorespancreaticobiliares
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Mutación del gen K-ras en tumores pancreático-biliares

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