Restauración de la inmunidad innata en pacientes con infección por VIH/SIDA después de inicio de terapia antirretroviral

Background: Highly active antiretroviral therapy (HAART) in HIV/AIDS infection induces an important reduction of the viral load (VL) and an immune system reconstitution. CD4+ T lymphocyte count is the immunological measurement commonly used for the follow up of HIV/AIDS patients. Aim: To study prosp...

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Autores principales:	Afani S,Alejandro, Jiusán L,Lorena, Raby A,Pablo, Sitia,Giovanni, Puente P,Javier, Sepúlveda C,Cecilia, Miranda W,Dante, Cabrera C,Roy, Guidotti,Luca, Lanza,Paola
Lenguaje:	Spanish / Castilian
Publicado:	Sociedad Médica de Santiago 2006
Materias:	Antiretroviral therapy, highly active CD4-positive T-lymphocytes HIV immunity, natural
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872006000600003
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spelling	oai:scielo:S0034-988720060006000032006-08-14Restauración de la inmunidad innata en pacientes con infección por VIH/SIDA después de inicio de terapia antirretroviralAfani S,AlejandroJiusán L,LorenaRaby A,PabloSitia,GiovanniPuente P,JavierSepúlveda C,CeciliaMiranda W,DanteCabrera C,RoyGuidotti,LucaLanza,Paola Antiretroviral therapy, highly active CD4-positive T-lymphocytes HIV immunity, natural Background: Highly active antiretroviral therapy (HAART) in HIV/AIDS infection induces an important reduction of the viral load (VL) and an immune system reconstitution. CD4+ T lymphocyte count is the immunological measurement commonly used for the follow up of HIV/AIDS patients. Aim: To study prospectively the restoration of the innate immune system in patients with HIV/AIDS infection during their first year on HAART. Patients and Methods: 25 naive HIV/AIDS patients, from San José Hospital and University of Chile Clinical Hospital, Santiago, Chile, were studied between years 2002-2003. Every 4 months after HAART initiation, CD3+, CD4+, CD8+ T lymphocytes and CD16/56+ natural killer (NK) cells were quantified by flow cytometry. NK cell cytotoxicity was measured using radioactive chrome liberation (Cr51). Tumor necrosis factor alpha (TNF-a) and interleukin-10 (IL-10) were measured in peripheral blood mononuclear cells and viral load was determined using Amplicor HIV-1 from Roche Diagnostics Systems. Results: Thirteen of the 25 patients continued in the study. They were all males, average age 35 years old (23-50). At baseline average CD4+ count was 146 cells/µL (31-362) and average viral load was 82.000 copies/mL (4.000-290.000). A raise in CD3+, CD4+, CD8+, and CD16/56 cells was noted at months 9-12 of therapy. Viral load became undetectable in the same period. NK cell function was decreased at the beginning of the therapy (1-4 months), reaching its highest values at months 9-12. There was no significant change in IL-10. TNF-a increased in six patients during the study. Conclusions: In this group of patients, innate immunity was restored during HAART. These results should be confirmed in studies with a longer follow up period and also measuring cytokines such as MIP-1a, MIP-1ß and RANTESinfo:eu-repo/semantics/openAccessSociedad Médica de SantiagoRevista médica de Chile v.134 n.6 20062006-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872006000600003es10.4067/S0034-98872006000600003
institution	Scielo Chile
collection	Scielo Chile
language	Spanish / Castilian
topic	Antiretroviral therapy, highly active CD4-positive T-lymphocytes HIV immunity, natural
spellingShingle	Antiretroviral therapy, highly active CD4-positive T-lymphocytes HIV immunity, natural Afani S,Alejandro Jiusán L,Lorena Raby A,Pablo Sitia,Giovanni Puente P,Javier Sepúlveda C,Cecilia Miranda W,Dante Cabrera C,Roy Guidotti,Luca Lanza,Paola Restauración de la inmunidad innata en pacientes con infección por VIH/SIDA después de inicio de terapia antirretroviral
description	Background: Highly active antiretroviral therapy (HAART) in HIV/AIDS infection induces an important reduction of the viral load (VL) and an immune system reconstitution. CD4+ T lymphocyte count is the immunological measurement commonly used for the follow up of HIV/AIDS patients. Aim: To study prospectively the restoration of the innate immune system in patients with HIV/AIDS infection during their first year on HAART. Patients and Methods: 25 naive HIV/AIDS patients, from San José Hospital and University of Chile Clinical Hospital, Santiago, Chile, were studied between years 2002-2003. Every 4 months after HAART initiation, CD3+, CD4+, CD8+ T lymphocytes and CD16/56+ natural killer (NK) cells were quantified by flow cytometry. NK cell cytotoxicity was measured using radioactive chrome liberation (Cr51). Tumor necrosis factor alpha (TNF-a) and interleukin-10 (IL-10) were measured in peripheral blood mononuclear cells and viral load was determined using Amplicor HIV-1 from Roche Diagnostics Systems. Results: Thirteen of the 25 patients continued in the study. They were all males, average age 35 years old (23-50). At baseline average CD4+ count was 146 cells/µL (31-362) and average viral load was 82.000 copies/mL (4.000-290.000). A raise in CD3+, CD4+, CD8+, and CD16/56 cells was noted at months 9-12 of therapy. Viral load became undetectable in the same period. NK cell function was decreased at the beginning of the therapy (1-4 months), reaching its highest values at months 9-12. There was no significant change in IL-10. TNF-a increased in six patients during the study. Conclusions: In this group of patients, innate immunity was restored during HAART. These results should be confirmed in studies with a longer follow up period and also measuring cytokines such as MIP-1a, MIP-1ß and RANTES
author	Afani S,Alejandro Jiusán L,Lorena Raby A,Pablo Sitia,Giovanni Puente P,Javier Sepúlveda C,Cecilia Miranda W,Dante Cabrera C,Roy Guidotti,Luca Lanza,Paola
author_facet	Afani S,Alejandro Jiusán L,Lorena Raby A,Pablo Sitia,Giovanni Puente P,Javier Sepúlveda C,Cecilia Miranda W,Dante Cabrera C,Roy Guidotti,Luca Lanza,Paola
author_sort	Afani S,Alejandro
title	Restauración de la inmunidad innata en pacientes con infección por VIH/SIDA después de inicio de terapia antirretroviral
title_short	Restauración de la inmunidad innata en pacientes con infección por VIH/SIDA después de inicio de terapia antirretroviral
title_full	Restauración de la inmunidad innata en pacientes con infección por VIH/SIDA después de inicio de terapia antirretroviral
title_fullStr	Restauración de la inmunidad innata en pacientes con infección por VIH/SIDA después de inicio de terapia antirretroviral
title_full_unstemmed	Restauración de la inmunidad innata en pacientes con infección por VIH/SIDA después de inicio de terapia antirretroviral
title_sort	restauración de la inmunidad innata en pacientes con infección por vih/sida después de inicio de terapia antirretroviral
publisher	Sociedad Médica de Santiago
publishDate	2006
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872006000600003
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Restauración de la inmunidad innata en pacientes con infección por VIH/SIDA después de inicio de terapia antirretroviral

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