Identificación rápida de micobacterias no tuberculosas mediante análisis de patrones de restricción

Background: The frequency of diseases caused by non tuberculous mycobacteria has increased in the last years. Their clinical diagnosis is difficult, mainly in immunocompromised patients. The identification of these mycobacteria by traditional methods is based on phenotypic characteristics and the re...

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Autores principales:	Araya R,Pamela, Velasco R,Maritza, Fernández O,Jorge
Lenguaje:	Spanish / Castilian
Publicado:	Sociedad Médica de Santiago 2006
Materias:	Mycobacteria, atypical Restriction enzyme analysis RNA, ribosomal
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872006000700010
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spelling	oai:scielo:S0034-988720060007000102006-08-29Identificación rápida de micobacterias no tuberculosas mediante análisis de patrones de restricciónAraya R,PamelaVelasco R,MaritzaFernández O,Jorge Mycobacteria, atypical Restriction enzyme analysis RNA, ribosomal Background: The frequency of diseases caused by non tuberculous mycobacteria has increased in the last years. Their clinical diagnosis is difficult, mainly in immunocompromised patients. The identification of these mycobacteria by traditional methods is based on phenotypic characteristics and the results are obtained two to four weeks after their isolation in primary cultures. Aim: To report a new identification method for non tuberculous mycobacteria. Material and methods: The restriction pattern analysis method was implemented. It is based on the amplification, using polymerase chain reaction (PCR), of a polymorphic region of 440 base pairs that codifies Hsp65 protein, followed by a digestion with BstE II and Hae III restriction enzymes. The results were compared with patterns established for each strain. Results: Sixty four strains of mycobacteria obtained from clinical samples and seven reference mycobacteria, were identified using the traditional methods and restriction pattern analysis. The latter method identified the same strain as the former in 87.5% of cases. In the remainder 12.5% of cases there was no agreement between both methods. In these, the sequencing of a fragment of a gene that codifies 16S ribosomal RNA, confirmed the correct identification by restriction patterns. Conclusions: Restriction pattern analysis is a rapid identification method for non tuberculous mycobaterial strainsinfo:eu-repo/semantics/openAccessSociedad Médica de SantiagoRevista médica de Chile v.134 n.7 20062006-07-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872006000700010es10.4067/S0034-98872006000700010
institution	Scielo Chile
collection	Scielo Chile
language	Spanish / Castilian
topic	Mycobacteria, atypical Restriction enzyme analysis RNA, ribosomal
spellingShingle	Mycobacteria, atypical Restriction enzyme analysis RNA, ribosomal Araya R,Pamela Velasco R,Maritza Fernández O,Jorge Identificación rápida de micobacterias no tuberculosas mediante análisis de patrones de restricción
description	Background: The frequency of diseases caused by non tuberculous mycobacteria has increased in the last years. Their clinical diagnosis is difficult, mainly in immunocompromised patients. The identification of these mycobacteria by traditional methods is based on phenotypic characteristics and the results are obtained two to four weeks after their isolation in primary cultures. Aim: To report a new identification method for non tuberculous mycobacteria. Material and methods: The restriction pattern analysis method was implemented. It is based on the amplification, using polymerase chain reaction (PCR), of a polymorphic region of 440 base pairs that codifies Hsp65 protein, followed by a digestion with BstE II and Hae III restriction enzymes. The results were compared with patterns established for each strain. Results: Sixty four strains of mycobacteria obtained from clinical samples and seven reference mycobacteria, were identified using the traditional methods and restriction pattern analysis. The latter method identified the same strain as the former in 87.5% of cases. In the remainder 12.5% of cases there was no agreement between both methods. In these, the sequencing of a fragment of a gene that codifies 16S ribosomal RNA, confirmed the correct identification by restriction patterns. Conclusions: Restriction pattern analysis is a rapid identification method for non tuberculous mycobaterial strains
author	Araya R,Pamela Velasco R,Maritza Fernández O,Jorge
author_facet	Araya R,Pamela Velasco R,Maritza Fernández O,Jorge
author_sort	Araya R,Pamela
title	Identificación rápida de micobacterias no tuberculosas mediante análisis de patrones de restricción
title_short	Identificación rápida de micobacterias no tuberculosas mediante análisis de patrones de restricción
title_full	Identificación rápida de micobacterias no tuberculosas mediante análisis de patrones de restricción
title_fullStr	Identificación rápida de micobacterias no tuberculosas mediante análisis de patrones de restricción
title_full_unstemmed	Identificación rápida de micobacterias no tuberculosas mediante análisis de patrones de restricción
title_sort	identificación rápida de micobacterias no tuberculosas mediante análisis de patrones de restricción
publisher	Sociedad Médica de Santiago
publishDate	2006
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872006000700010
work_keys_str_mv	AT arayarpamela identificacionrapidademicobacteriasnotuberculosasmedianteanalisisdepatronesderestriccion AT velascormaritza identificacionrapidademicobacteriasnotuberculosasmedianteanalisisdepatronesderestriccion AT fernandezojorge identificacionrapidademicobacteriasnotuberculosasmedianteanalisisdepatronesderestriccion
_version_	1718436270985707520

Identificación rápida de micobacterias no tuberculosas mediante análisis de patrones de restricción

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