Genotipificación de aislados clínicos de Helicobacter pylori en base a genes asociados a virulencia cagA, vacA y babA2: Primer aislamiento de una cepa babA2 positiva en pacientes chilenos

Background: Helicobacter pylori-associated gastroduodenal diseases depends on host characteristics, environmental conditions and bacterial virulence factors, such as cagA, vacA y babA2 gene products. Moreover, peptic ulcer disease has been related with cagA+, vacAs1m1 strains, while metaplasia and g...

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Autores principales:	García C,Apolinaria, Barra T,Ricardo, Delgado Sch,Carolina, Kawaguchi P,Fernando, Trabal F,Natalia, Montenegro H,Sonia, González C,Carlos
Lenguaje:	Spanish / Castilian
Publicado:	Sociedad Médica de Santiago 2006
Materias:	babA protein, Helicobacter pylori cagA protein, Helicobacter pylori Helicobacter pylori vacA protein, Helicobacter pylori
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872006000800006
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spelling	oai:scielo:S0034-988720060008000062014-01-23Genotipificación de aislados clínicos de Helicobacter pylori en base a genes asociados a virulencia cagA, vacA y babA2: Primer aislamiento de una cepa babA2 positiva en pacientes chilenosGarcía C,ApolinariaBarra T,RicardoDelgado Sch,CarolinaKawaguchi P,FernandoTrabal F,NataliaMontenegro H,SoniaGonzález C,Carlos babA protein, Helicobacter pylori cagA protein, Helicobacter pylori Helicobacter pylori vacA protein, Helicobacter pylori Background: Helicobacter pylori-associated gastroduodenal diseases depends on host characteristics, environmental conditions and bacterial virulence factors, such as cagA, vacA y babA2 gene products. Moreover, peptic ulcer disease has been related with cagA+, vacAs1m1 strains, while metaplasia and gastric cancer has been associated to cagA+, vacAs1 and babA2+ H pylori strains. Gene babA2 has not yet been described in clinical isolates from Chilean patients. Aim: To investigate the presence of cagA, vacA (s and m) and babA2 genes in clinical isolates of H pylori from Chilean patients. Material and Methods: Sixty six isolates from 41 patients were genotyped by PCR, using primers for s1a, s1b, s2, m1, m2, cagA and babA2 genes as previously described. Results: cagA gene was detected in 16 isolates (24.2%) while vacAs1a, vacAs1b, vacAs2, vacAm1 and vacAm2 were detected in 28 (42.4%), 14 (21.2%), 17 (25.8%), 21 (31.8%) and 29 isolates (43.9%), respectively. One isolate (1.5%) was babA2 positive, being the first isolate with this genotype described in Chile. Besides the babA2+ genotype this clinical isolate also presented cagA+ and vacAs1a which has been related with metaplasia or gastric cancer. Five isolates showed an ulcerogenic profile cagA+, vacAs1m1. Conclusions: The results presented indicate the prevalence of vacAs1m1 genotype among the clinical isolates analyzed, and a low frequency of babA2 genotype (Rev Méd Chile 2006; 134: 981-8).info:eu-repo/semantics/openAccessSociedad Médica de SantiagoRevista médica de Chile v.134 n.8 20062006-08-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872006000800006es10.4067/S0034-98872006000800006
institution	Scielo Chile
collection	Scielo Chile
language	Spanish / Castilian
topic	babA protein, Helicobacter pylori cagA protein, Helicobacter pylori Helicobacter pylori vacA protein, Helicobacter pylori
spellingShingle	babA protein, Helicobacter pylori cagA protein, Helicobacter pylori Helicobacter pylori vacA protein, Helicobacter pylori García C,Apolinaria Barra T,Ricardo Delgado Sch,Carolina Kawaguchi P,Fernando Trabal F,Natalia Montenegro H,Sonia González C,Carlos Genotipificación de aislados clínicos de Helicobacter pylori en base a genes asociados a virulencia cagA, vacA y babA2: Primer aislamiento de una cepa babA2 positiva en pacientes chilenos
description	Background: Helicobacter pylori-associated gastroduodenal diseases depends on host characteristics, environmental conditions and bacterial virulence factors, such as cagA, vacA y babA2 gene products. Moreover, peptic ulcer disease has been related with cagA+, vacAs1m1 strains, while metaplasia and gastric cancer has been associated to cagA+, vacAs1 and babA2+ H pylori strains. Gene babA2 has not yet been described in clinical isolates from Chilean patients. Aim: To investigate the presence of cagA, vacA (s and m) and babA2 genes in clinical isolates of H pylori from Chilean patients. Material and Methods: Sixty six isolates from 41 patients were genotyped by PCR, using primers for s1a, s1b, s2, m1, m2, cagA and babA2 genes as previously described. Results: cagA gene was detected in 16 isolates (24.2%) while vacAs1a, vacAs1b, vacAs2, vacAm1 and vacAm2 were detected in 28 (42.4%), 14 (21.2%), 17 (25.8%), 21 (31.8%) and 29 isolates (43.9%), respectively. One isolate (1.5%) was babA2 positive, being the first isolate with this genotype described in Chile. Besides the babA2+ genotype this clinical isolate also presented cagA+ and vacAs1a which has been related with metaplasia or gastric cancer. Five isolates showed an ulcerogenic profile cagA+, vacAs1m1. Conclusions: The results presented indicate the prevalence of vacAs1m1 genotype among the clinical isolates analyzed, and a low frequency of babA2 genotype (Rev Méd Chile 2006; 134: 981-8).
author	García C,Apolinaria Barra T,Ricardo Delgado Sch,Carolina Kawaguchi P,Fernando Trabal F,Natalia Montenegro H,Sonia González C,Carlos
author_facet	García C,Apolinaria Barra T,Ricardo Delgado Sch,Carolina Kawaguchi P,Fernando Trabal F,Natalia Montenegro H,Sonia González C,Carlos
author_sort	García C,Apolinaria
title	Genotipificación de aislados clínicos de Helicobacter pylori en base a genes asociados a virulencia cagA, vacA y babA2: Primer aislamiento de una cepa babA2 positiva en pacientes chilenos
title_short	Genotipificación de aislados clínicos de Helicobacter pylori en base a genes asociados a virulencia cagA, vacA y babA2: Primer aislamiento de una cepa babA2 positiva en pacientes chilenos
title_full	Genotipificación de aislados clínicos de Helicobacter pylori en base a genes asociados a virulencia cagA, vacA y babA2: Primer aislamiento de una cepa babA2 positiva en pacientes chilenos
title_fullStr	Genotipificación de aislados clínicos de Helicobacter pylori en base a genes asociados a virulencia cagA, vacA y babA2: Primer aislamiento de una cepa babA2 positiva en pacientes chilenos
title_full_unstemmed	Genotipificación de aislados clínicos de Helicobacter pylori en base a genes asociados a virulencia cagA, vacA y babA2: Primer aislamiento de una cepa babA2 positiva en pacientes chilenos
title_sort	genotipificación de aislados clínicos de helicobacter pylori en base a genes asociados a virulencia caga, vaca y baba2: primer aislamiento de una cepa baba2 positiva en pacientes chilenos
publisher	Sociedad Médica de Santiago
publishDate	2006
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872006000800006
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Genotipificación de aislados clínicos de Helicobacter pylori en base a genes asociados a virulencia cagA, vacA y babA2: Primer aislamiento de una cepa babA2 positiva en pacientes chilenos

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