Genotipos de Staphylococcus aureus con fenotipo meticilino resistente, aislados de pacientes del Hospital Base de Valdivia

Background: Methicillin resistant strains of Staphylococcus aureus (MRSA) are an important cause of nosocomial infections. Aim: To determine the genotypes of MRSA strains. Material and methods: Fifty five strains of MRSA, isolated from patients hospitalized in Hospital Base Valdivia, were studied. T...

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Autores principales:	Wilson S,Myra, Otth L,Carola, Medina S,Gustavo, Otth R,Laura, Fernández J,Heriberto, Arce,María, Zaror C,Angela, Lizama,Víctor, Gil D,Mónica, von Chrismar,Ana María
Lenguaje:	Spanish / Castilian
Publicado:	Sociedad Médica de Santiago 2007
Materias:	Drug resistance, bacterial Methicillin Staphylococcu aureus
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872007000500007
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spelling	oai:scielo:S0034-988720070005000072007-07-09Genotipos de Staphylococcus aureus con fenotipo meticilino resistente, aislados de pacientes del Hospital Base de ValdiviaWilson S,MyraOtth L,CarolaMedina S,GustavoOtth R,LauraFernández J,HeribertoArce,MaríaZaror C,AngelaLizama,VíctorGil D,Mónicavon Chrismar,Ana María Drug resistance, bacterial Methicillin Staphylococcu aureus Background: Methicillin resistant strains of Staphylococcus aureus (MRSA) are an important cause of nosocomial infections. Aim: To determine the genotypes of MRSA strains. Material and methods: Fifty five strains of MRSA, isolated from patients hospitalized in Hospital Base Valdivia, were studied. The phenotype was determined through MicroScan® in all strains and by minimum inhibitory concentration (MIC) in 41. The genotype of the strains was analyzed by a duplex polymerase chain reaction (PCR) of the mecA gene, amplifying eight hypervariable DNA regions associated to such gene. Results: According to MIC, 88% of strains had a pattern of resistance against multiple antimicrobial (penicillin, ampicillin, cephradine, gentamycin, ciprofloxacin, ¡incomycin and erythromycin). Vancomicin resistan strains were not detected. Only 53 strains (96%) had at least one of the eight hypervariable regions and were classified as MRSA. Genotypic patterns types 15 were the most commonly detected in 38% and 34% of strains, respectively. MicroScan® erroneously classified five strains in an incorrect phenotype, according to results obtained with duplex PCR. MIC results did not differ from those of duplex PCR. Conclusions: Duplex- PCR is a useful tool to detect hyper variable regions associated to mecA geneinfo:eu-repo/semantics/openAccessSociedad Médica de SantiagoRevista médica de Chile v.135 n.5 20072007-05-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872007000500007es10.4067/S0034-98872007000500007
institution	Scielo Chile
collection	Scielo Chile
language	Spanish / Castilian
topic	Drug resistance, bacterial Methicillin Staphylococcu aureus
spellingShingle	Drug resistance, bacterial Methicillin Staphylococcu aureus Wilson S,Myra Otth L,Carola Medina S,Gustavo Otth R,Laura Fernández J,Heriberto Arce,María Zaror C,Angela Lizama,Víctor Gil D,Mónica von Chrismar,Ana María Genotipos de Staphylococcus aureus con fenotipo meticilino resistente, aislados de pacientes del Hospital Base de Valdivia
description	Background: Methicillin resistant strains of Staphylococcus aureus (MRSA) are an important cause of nosocomial infections. Aim: To determine the genotypes of MRSA strains. Material and methods: Fifty five strains of MRSA, isolated from patients hospitalized in Hospital Base Valdivia, were studied. The phenotype was determined through MicroScan® in all strains and by minimum inhibitory concentration (MIC) in 41. The genotype of the strains was analyzed by a duplex polymerase chain reaction (PCR) of the mecA gene, amplifying eight hypervariable DNA regions associated to such gene. Results: According to MIC, 88% of strains had a pattern of resistance against multiple antimicrobial (penicillin, ampicillin, cephradine, gentamycin, ciprofloxacin, ¡incomycin and erythromycin). Vancomicin resistan strains were not detected. Only 53 strains (96%) had at least one of the eight hypervariable regions and were classified as MRSA. Genotypic patterns types 15 were the most commonly detected in 38% and 34% of strains, respectively. MicroScan® erroneously classified five strains in an incorrect phenotype, according to results obtained with duplex PCR. MIC results did not differ from those of duplex PCR. Conclusions: Duplex- PCR is a useful tool to detect hyper variable regions associated to mecA gene
author	Wilson S,Myra Otth L,Carola Medina S,Gustavo Otth R,Laura Fernández J,Heriberto Arce,María Zaror C,Angela Lizama,Víctor Gil D,Mónica von Chrismar,Ana María
author_facet	Wilson S,Myra Otth L,Carola Medina S,Gustavo Otth R,Laura Fernández J,Heriberto Arce,María Zaror C,Angela Lizama,Víctor Gil D,Mónica von Chrismar,Ana María
author_sort	Wilson S,Myra
title	Genotipos de Staphylococcus aureus con fenotipo meticilino resistente, aislados de pacientes del Hospital Base de Valdivia
title_short	Genotipos de Staphylococcus aureus con fenotipo meticilino resistente, aislados de pacientes del Hospital Base de Valdivia
title_full	Genotipos de Staphylococcus aureus con fenotipo meticilino resistente, aislados de pacientes del Hospital Base de Valdivia
title_fullStr	Genotipos de Staphylococcus aureus con fenotipo meticilino resistente, aislados de pacientes del Hospital Base de Valdivia
title_full_unstemmed	Genotipos de Staphylococcus aureus con fenotipo meticilino resistente, aislados de pacientes del Hospital Base de Valdivia
title_sort	genotipos de staphylococcus aureus con fenotipo meticilino resistente, aislados de pacientes del hospital base de valdivia
publisher	Sociedad Médica de Santiago
publishDate	2007
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872007000500007
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Genotipos de Staphylococcus aureus con fenotipo meticilino resistente, aislados de pacientes del Hospital Base de Valdivia

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