Metabolismo del glutatión y desarrollo posnatal del pulmón de la rata

Background: Significant changes in lung antioxidants occur in preparation for birth. Little is known about physiological regulation of antioxidants in the postnatal period. Aim: To study the glutathione system in the lungs during postnatal development. Material and methods: In the lungs of 7, 15, 21...

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Autores principales:	Ramírez,Alejandro, Reyes,Tatiana, Liberona,Leonel, Bustamente,Andrea, Sáez,Claudia, Borzone,Gisella
Lenguaje:	Spanish / Castilian
Publicado:	Sociedad Médica de Santiago 2007
Materias:	Antioxidants Glutathione Growth and development
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872007000700010
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spelling	oai:scielo:S0034-988720070007000102007-09-06Metabolismo del glutatión y desarrollo posnatal del pulmón de la rataRamírez,AlejandroReyes,TatianaLiberona,LeonelBustamente,AndreaSáez,ClaudiaBorzone,Gisella Antioxidants Glutathione Growth and development Background: Significant changes in lung antioxidants occur in preparation for birth. Little is known about physiological regulation of antioxidants in the postnatal period. Aim: To study the glutathione system in the lungs during postnatal development. Material and methods: In the lungs of 7, 15, 21, 50 and 70 days old Sprague-Dawley rats we measured total and oxidized glutathione content as well as the activity of the limiting enzyme in glutathione synthesis (y-GCS) and of the enzymes glutathione peroxidase (GPx) and glutathione reducíase (GRd). Results: Between 7 and 15 days the activities of GPx and GRd increase 32% and 26%, respectively (p <0.001). Whereas GPx activity remains high throughout the rest of the study period, GRd activity decreases progressively reaching adulthood values at 7 days. y-GCS activity shows a gradual increase that reaches significance at 50 days when it doubles values observed at 7 days (p <0.05). A significant correlation was found between GPx and GRd activities over the entire period (r =0.62, p <0.0001). Strength of the correlation is age dependent due to the differences in time course of the enzyme changes. Whereas total GSH does not change, oxidized glutathione decreases from 7% at 7 and 15 days to 4% later on (p <0.01). Conclusions: The activity of several enzymes involved in glutathione metabolism increases during postnatal development of the rat lung. Interpretation of lung responses to injurious agents needs to be done taking into consideration the physiological regulation of antioxidants during postnatal developmentinfo:eu-repo/semantics/openAccessSociedad Médica de SantiagoRevista médica de Chile v.135 n.7 20072007-07-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872007000700010es10.4067/S0034-98872007000700010
institution	Scielo Chile
collection	Scielo Chile
language	Spanish / Castilian
topic	Antioxidants Glutathione Growth and development
spellingShingle	Antioxidants Glutathione Growth and development Ramírez,Alejandro Reyes,Tatiana Liberona,Leonel Bustamente,Andrea Sáez,Claudia Borzone,Gisella Metabolismo del glutatión y desarrollo posnatal del pulmón de la rata
description	Background: Significant changes in lung antioxidants occur in preparation for birth. Little is known about physiological regulation of antioxidants in the postnatal period. Aim: To study the glutathione system in the lungs during postnatal development. Material and methods: In the lungs of 7, 15, 21, 50 and 70 days old Sprague-Dawley rats we measured total and oxidized glutathione content as well as the activity of the limiting enzyme in glutathione synthesis (y-GCS) and of the enzymes glutathione peroxidase (GPx) and glutathione reducíase (GRd). Results: Between 7 and 15 days the activities of GPx and GRd increase 32% and 26%, respectively (p <0.001). Whereas GPx activity remains high throughout the rest of the study period, GRd activity decreases progressively reaching adulthood values at 7 days. y-GCS activity shows a gradual increase that reaches significance at 50 days when it doubles values observed at 7 days (p <0.05). A significant correlation was found between GPx and GRd activities over the entire period (r =0.62, p <0.0001). Strength of the correlation is age dependent due to the differences in time course of the enzyme changes. Whereas total GSH does not change, oxidized glutathione decreases from 7% at 7 and 15 days to 4% later on (p <0.01). Conclusions: The activity of several enzymes involved in glutathione metabolism increases during postnatal development of the rat lung. Interpretation of lung responses to injurious agents needs to be done taking into consideration the physiological regulation of antioxidants during postnatal development
author	Ramírez,Alejandro Reyes,Tatiana Liberona,Leonel Bustamente,Andrea Sáez,Claudia Borzone,Gisella
author_facet	Ramírez,Alejandro Reyes,Tatiana Liberona,Leonel Bustamente,Andrea Sáez,Claudia Borzone,Gisella
author_sort	Ramírez,Alejandro
title	Metabolismo del glutatión y desarrollo posnatal del pulmón de la rata
title_short	Metabolismo del glutatión y desarrollo posnatal del pulmón de la rata
title_full	Metabolismo del glutatión y desarrollo posnatal del pulmón de la rata
title_fullStr	Metabolismo del glutatión y desarrollo posnatal del pulmón de la rata
title_full_unstemmed	Metabolismo del glutatión y desarrollo posnatal del pulmón de la rata
title_sort	metabolismo del glutatión y desarrollo posnatal del pulmón de la rata
publisher	Sociedad Médica de Santiago
publishDate	2007
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872007000700010
work_keys_str_mv	AT ramirezalejandro metabolismodelglutationydesarrolloposnataldelpulmondelarata AT reyestatiana metabolismodelglutationydesarrolloposnataldelpulmondelarata AT liberonaleonel metabolismodelglutationydesarrolloposnataldelpulmondelarata AT bustamenteandrea metabolismodelglutationydesarrolloposnataldelpulmondelarata AT saezclaudia metabolismodelglutationydesarrolloposnataldelpulmondelarata AT borzonegisella metabolismodelglutationydesarrolloposnataldelpulmondelarata
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Metabolismo del glutatión y desarrollo posnatal del pulmón de la rata

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