Presencia de ADN bacteriano en el tejido valvular de pacientes con cardiopatía reumática crónica

Background: Rheumatic heart disease (RHD) is a delayed consequence of a pharyngeal infection with Group A streptococcus (GAS), usually ascribed to a cross-reactive immune response to the host cardiac tissues. Acute rheumatic fever (ARF) and its ensuing valvular sequelae are thus considered the proto...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Figueroa,Fernando E, Carrión A,Flavio, Valenzuela M,Sylvia, Turner G,Eduardo, Aceitón E,Cristian, Hirigoyen P,Carolina, Bogdanic W,Katherine, Solís D,Claudia, Mansilla A,Karina, Urra G,Soledad
Lenguaje:	Spanish / Castilian
Publicado:	Sociedad Médica de Santiago 2007
Materias:	DNA bacterial Rheumatic heart disease Streptococcus
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872007000800001
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0034-98872007000800001
record_format	dspace
spelling	oai:scielo:S0034-988720070008000012007-10-25Presencia de ADN bacteriano en el tejido valvular de pacientes con cardiopatía reumática crónicaFigueroa,Fernando ECarrión A,FlavioValenzuela M,SylviaTurner G,EduardoAceitón E,CristianHirigoyen P,CarolinaBogdanic W,KatherineSolís D,ClaudiaMansilla A,KarinaUrra G,Soledad DNA bacterial Rheumatic heart disease Streptococcus Background: Rheumatic heart disease (RHD) is a delayed consequence of a pharyngeal infection with Group A streptococcus (GAS), usually ascribed to a cross-reactive immune response to the host cardiac tissues. Acute rheumatic fever (ARF) and its ensuing valvular sequelae are thus considered the prototype of a post-infectious autoimmune disease, with no direct evidence of residual streptococcal antigen in diseased valvular tissues. However, recent studies concerning the antigenic specificity and clonality of intralesional lymphocytes have revealed oligoclonal expansions characteristic of an antigen specific response, that might be related to GAS. Aim: To search for bacterial DNA in valvular tissue from RHD patients and controls. Material and methods: We extracted DNA from surgically excised valve specimens from 15 RHD patients and 6 non RHD controls and tested for the presence of bacterial DNA by Polymerase Chain Reaction (PCR) with primers for 16S rRNA. Results: Eighty percent (12/15) of valve specimens from RHD patients were positive for bacterial DNA, as opposed to none of the valves (n =6) from non RHD controls. Conclusions: These results suggest that GAS might persist in valvular tissue in patients with ARF and contribute to the inflammatory scarring lesion that leads to cardiovascular sequelae (Rev Méd Chile 2007; 135: 959-66)info:eu-repo/semantics/openAccessSociedad Médica de SantiagoRevista médica de Chile v.135 n.8 20072007-08-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872007000800001es10.4067/S0034-98872007000800001
institution	Scielo Chile
collection	Scielo Chile
language	Spanish / Castilian
topic	DNA bacterial Rheumatic heart disease Streptococcus
spellingShingle	DNA bacterial Rheumatic heart disease Streptococcus Figueroa,Fernando E Carrión A,Flavio Valenzuela M,Sylvia Turner G,Eduardo Aceitón E,Cristian Hirigoyen P,Carolina Bogdanic W,Katherine Solís D,Claudia Mansilla A,Karina Urra G,Soledad Presencia de ADN bacteriano en el tejido valvular de pacientes con cardiopatía reumática crónica
description	Background: Rheumatic heart disease (RHD) is a delayed consequence of a pharyngeal infection with Group A streptococcus (GAS), usually ascribed to a cross-reactive immune response to the host cardiac tissues. Acute rheumatic fever (ARF) and its ensuing valvular sequelae are thus considered the prototype of a post-infectious autoimmune disease, with no direct evidence of residual streptococcal antigen in diseased valvular tissues. However, recent studies concerning the antigenic specificity and clonality of intralesional lymphocytes have revealed oligoclonal expansions characteristic of an antigen specific response, that might be related to GAS. Aim: To search for bacterial DNA in valvular tissue from RHD patients and controls. Material and methods: We extracted DNA from surgically excised valve specimens from 15 RHD patients and 6 non RHD controls and tested for the presence of bacterial DNA by Polymerase Chain Reaction (PCR) with primers for 16S rRNA. Results: Eighty percent (12/15) of valve specimens from RHD patients were positive for bacterial DNA, as opposed to none of the valves (n =6) from non RHD controls. Conclusions: These results suggest that GAS might persist in valvular tissue in patients with ARF and contribute to the inflammatory scarring lesion that leads to cardiovascular sequelae (Rev Méd Chile 2007; 135: 959-66)
author	Figueroa,Fernando E Carrión A,Flavio Valenzuela M,Sylvia Turner G,Eduardo Aceitón E,Cristian Hirigoyen P,Carolina Bogdanic W,Katherine Solís D,Claudia Mansilla A,Karina Urra G,Soledad
author_facet	Figueroa,Fernando E Carrión A,Flavio Valenzuela M,Sylvia Turner G,Eduardo Aceitón E,Cristian Hirigoyen P,Carolina Bogdanic W,Katherine Solís D,Claudia Mansilla A,Karina Urra G,Soledad
author_sort	Figueroa,Fernando E
title	Presencia de ADN bacteriano en el tejido valvular de pacientes con cardiopatía reumática crónica
title_short	Presencia de ADN bacteriano en el tejido valvular de pacientes con cardiopatía reumática crónica
title_full	Presencia de ADN bacteriano en el tejido valvular de pacientes con cardiopatía reumática crónica
title_fullStr	Presencia de ADN bacteriano en el tejido valvular de pacientes con cardiopatía reumática crónica
title_full_unstemmed	Presencia de ADN bacteriano en el tejido valvular de pacientes con cardiopatía reumática crónica
title_sort	presencia de adn bacteriano en el tejido valvular de pacientes con cardiopatía reumática crónica
publisher	Sociedad Médica de Santiago
publishDate	2007
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872007000800001
work_keys_str_mv	AT figueroafernandoe presenciadeadnbacterianoeneltejidovalvulardepacientesconcardiopatiareumaticacronica AT carrionaflavio presenciadeadnbacterianoeneltejidovalvulardepacientesconcardiopatiareumaticacronica AT valenzuelamsylvia presenciadeadnbacterianoeneltejidovalvulardepacientesconcardiopatiareumaticacronica AT turnergeduardo presenciadeadnbacterianoeneltejidovalvulardepacientesconcardiopatiareumaticacronica AT aceitonecristian presenciadeadnbacterianoeneltejidovalvulardepacientesconcardiopatiareumaticacronica AT hirigoyenpcarolina presenciadeadnbacterianoeneltejidovalvulardepacientesconcardiopatiareumaticacronica AT bogdanicwkatherine presenciadeadnbacterianoeneltejidovalvulardepacientesconcardiopatiareumaticacronica AT solisdclaudia presenciadeadnbacterianoeneltejidovalvulardepacientesconcardiopatiareumaticacronica AT mansillaakarina presenciadeadnbacterianoeneltejidovalvulardepacientesconcardiopatiareumaticacronica AT urragsoledad presenciadeadnbacterianoeneltejidovalvulardepacientesconcardiopatiareumaticacronica
_version_	1718436331955159040

Presencia de ADN bacteriano en el tejido valvular de pacientes con cardiopatía reumática crónica

Ejemplares similares