Epidemiología clínica y molecular de las infecciones por Shigella spp en niños de la Región Metropolitana durante el verano 2004-2005

Background: Shigella spp is a frequent cause of diarrhea in children. Antimicrobials decrease the duration of diarrhea and pathogen excretion. However, the increasing resistance limits their therapeutic value. Aim: To study Shigella serotype distribution in the Metropolitan Region in Chile, and its...

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Autores principales:	Hamilton-West M,Christopher, Prado J,Valeria, Hormazábal O,Juan Carlos, Lagos Z,Rosanna, Benadof F,Dona, Mendoza N,Carmen, Elgueta N,Andrea, Tapia P,Cecilia, C¡fuentes D,Marcela, Alvarez A,Isabel, Hernández E,Mariluz
Lenguaje:	Spanish / Castilian
Publicado:	Sociedad Médica de Santiago 2007
Materias:	Anti-bacterial agents Diarrhea, infantile Shigella
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872007001100004
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spelling	oai:scielo:S0034-988720070011000042008-02-08Epidemiología clínica y molecular de las infecciones por Shigella spp en niños de la Región Metropolitana durante el verano 2004-2005Hamilton-West M,ChristopherPrado J,ValeriaHormazábal O,Juan CarlosLagos Z,RosannaBenadof F,DonaMendoza N,CarmenElgueta N,AndreaTapia P,CeciliaC¡fuentes D,MarcelaAlvarez A,IsabelHernández E,Mariluz Anti-bacterial agents Diarrhea, infantile Shigella Background: Shigella spp is a frequent cause of diarrhea in children. Antimicrobials decrease the duration of diarrhea and pathogen excretion. However, the increasing resistance limits their therapeutic value. Aim: To study Shigella serotype distribution in the Metropolitan Region in Chile, and its relationship with severity of disease, antimicrobial resistance pattern and clonality. Material and methods: During summer 2004-2005, stool samples from children with diarrhea were collected in Cary Blair transpon medium and cultured. Shigella isolates were serotyped using monoclonal and polyclonal commercial antibodies. In vitro activity of ampicillin, amoxicillin/clavulanic acid, chloramphenicol, cotrimoxazol, nalidixic acid, ciprofloxacin, ceftriaxone and azythromycin was determined by minimal inhibitory concentration (MIC). Clonality was studied by pulsed-field gel electrophoresis (PFGE) using Xbal as restriction enzyme. Results: One hundred thirty nine Shigella strains were isolated (77 S sonnei and 62 S flexneri). S sonnei and S flexneri 2a serotypes were responsible for 95% of episodes. Children aged 2-4 years, showed a greater incidence of Shigella infections and 77% of episodes were treated on an ambulatory basis. High resistance levels were observed for ampicillin, cotrimoxazole, amoxicillin-clavulanic acid and chloramphenicol (67%, 60%, 56% and 45%, respectively). We found 11 resistance patterns and 61,2% of strains were multiresistant. There were multiple clones without a strict relationship with resistance patterns. Conclusions: Shigella infections in Metropolitan Region in Chile are associated to a restricted number of serotypes, representing a clonal expansion associated to different antimicrobial resistant patternsinfo:eu-repo/semantics/openAccessSociedad Médica de SantiagoRevista médica de Chile v.135 n.11 20072007-11-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872007001100004es10.4067/S0034-98872007001100004
institution	Scielo Chile
collection	Scielo Chile
language	Spanish / Castilian
topic	Anti-bacterial agents Diarrhea, infantile Shigella
spellingShingle	Anti-bacterial agents Diarrhea, infantile Shigella Hamilton-West M,Christopher Prado J,Valeria Hormazábal O,Juan Carlos Lagos Z,Rosanna Benadof F,Dona Mendoza N,Carmen Elgueta N,Andrea Tapia P,Cecilia C¡fuentes D,Marcela Alvarez A,Isabel Hernández E,Mariluz Epidemiología clínica y molecular de las infecciones por Shigella spp en niños de la Región Metropolitana durante el verano 2004-2005
description	Background: Shigella spp is a frequent cause of diarrhea in children. Antimicrobials decrease the duration of diarrhea and pathogen excretion. However, the increasing resistance limits their therapeutic value. Aim: To study Shigella serotype distribution in the Metropolitan Region in Chile, and its relationship with severity of disease, antimicrobial resistance pattern and clonality. Material and methods: During summer 2004-2005, stool samples from children with diarrhea were collected in Cary Blair transpon medium and cultured. Shigella isolates were serotyped using monoclonal and polyclonal commercial antibodies. In vitro activity of ampicillin, amoxicillin/clavulanic acid, chloramphenicol, cotrimoxazol, nalidixic acid, ciprofloxacin, ceftriaxone and azythromycin was determined by minimal inhibitory concentration (MIC). Clonality was studied by pulsed-field gel electrophoresis (PFGE) using Xbal as restriction enzyme. Results: One hundred thirty nine Shigella strains were isolated (77 S sonnei and 62 S flexneri). S sonnei and S flexneri 2a serotypes were responsible for 95% of episodes. Children aged 2-4 years, showed a greater incidence of Shigella infections and 77% of episodes were treated on an ambulatory basis. High resistance levels were observed for ampicillin, cotrimoxazole, amoxicillin-clavulanic acid and chloramphenicol (67%, 60%, 56% and 45%, respectively). We found 11 resistance patterns and 61,2% of strains were multiresistant. There were multiple clones without a strict relationship with resistance patterns. Conclusions: Shigella infections in Metropolitan Region in Chile are associated to a restricted number of serotypes, representing a clonal expansion associated to different antimicrobial resistant patterns
author	Hamilton-West M,Christopher Prado J,Valeria Hormazábal O,Juan Carlos Lagos Z,Rosanna Benadof F,Dona Mendoza N,Carmen Elgueta N,Andrea Tapia P,Cecilia C¡fuentes D,Marcela Alvarez A,Isabel Hernández E,Mariluz
author_facet	Hamilton-West M,Christopher Prado J,Valeria Hormazábal O,Juan Carlos Lagos Z,Rosanna Benadof F,Dona Mendoza N,Carmen Elgueta N,Andrea Tapia P,Cecilia C¡fuentes D,Marcela Alvarez A,Isabel Hernández E,Mariluz
author_sort	Hamilton-West M,Christopher
title	Epidemiología clínica y molecular de las infecciones por Shigella spp en niños de la Región Metropolitana durante el verano 2004-2005
title_short	Epidemiología clínica y molecular de las infecciones por Shigella spp en niños de la Región Metropolitana durante el verano 2004-2005
title_full	Epidemiología clínica y molecular de las infecciones por Shigella spp en niños de la Región Metropolitana durante el verano 2004-2005
title_fullStr	Epidemiología clínica y molecular de las infecciones por Shigella spp en niños de la Región Metropolitana durante el verano 2004-2005
title_full_unstemmed	Epidemiología clínica y molecular de las infecciones por Shigella spp en niños de la Región Metropolitana durante el verano 2004-2005
title_sort	epidemiología clínica y molecular de las infecciones por shigella spp en niños de la región metropolitana durante el verano 2004-2005
publisher	Sociedad Médica de Santiago
publishDate	2007
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872007001100004
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Epidemiología clínica y molecular de las infecciones por Shigella spp en niños de la Región Metropolitana durante el verano 2004-2005

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