Actividad supresora del millerenólido sobre células mononucleares de sangre periférica humana

Background: Natural products are used in the production of therapeutic drugs due to their wide diversity and excellent adaptability to biological structures. Sesquiterpene ¡aciones are the active constituents of several plants from the Asteraceae family. Aim: To assess the in vitro effect of a sesqu...

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Autores principales:	Dupuy L,Ornar A, Murillo,Renato, Bonilla V,José A
Lenguaje:	Spanish / Castilian
Publicado:	Sociedad Médica de Santiago 2008
Materias:	Immunosuppression Lymphocytes Sesquiterpenes
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872008000100008
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spelling	oai:scielo:S0034-988720080001000082008-04-30Actividad supresora del millerenólido sobre células mononucleares de sangre periférica humanaDupuy L,Ornar AMurillo,RenatoBonilla V,José A Immunosuppression Lymphocytes Sesquiterpenes Background: Natural products are used in the production of therapeutic drugs due to their wide diversity and excellent adaptability to biological structures. Sesquiterpene ¡aciones are the active constituents of several plants from the Asteraceae family. Aim: To assess the in vitro effect of a sesquiterpene lactone (millerenolide). Material and methods: The drug effect was assessed measuring the proliferation of lymphocytes using the 2,3-bis (2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino) carbonylJ-2H-tetrazolium hydroxide (XTT) technique. Changes on the cell cycle were analyzed on a FACSort flow cytometer The effect of millerenolide on the production of nitric oxide (NO) by macrophages was evaluated using the Griess reagent. Additionally, phagocytosis of latex particles and nitroblue tetrazolium (NBT) reduction by macrophages were evaluated microscopically. Results: Treatment of human peripheral blood mononuclear cells (PBMC) with millerenolide decreases the proliferation of lymphocytes, decreases the percentage of cells in S, and G2/Mphases, and increases the proportion of cells in GO/Gl phase. Treatment of macrophages with millerenolide, reduces the production of NO, the phagocytic capacity and the number of cells able to reduce NBT. Cytotoxic effects of the lactone on human PBMC were only observed when the concentration was increased to 6 fig/ml. Conclusions: Millerenolide could be considered as a potential therapeutic agent with immunosuppressiveproperties (RevMéd Chile 2007; 135: 64-72)info:eu-repo/semantics/openAccessSociedad Médica de SantiagoRevista médica de Chile v.136 n.1 20082008-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872008000100008es10.4067/S0034-98872008000100008
institution	Scielo Chile
collection	Scielo Chile
language	Spanish / Castilian
topic	Immunosuppression Lymphocytes Sesquiterpenes
spellingShingle	Immunosuppression Lymphocytes Sesquiterpenes Dupuy L,Ornar A Murillo,Renato Bonilla V,José A Actividad supresora del millerenólido sobre células mononucleares de sangre periférica humana
description	Background: Natural products are used in the production of therapeutic drugs due to their wide diversity and excellent adaptability to biological structures. Sesquiterpene ¡aciones are the active constituents of several plants from the Asteraceae family. Aim: To assess the in vitro effect of a sesquiterpene lactone (millerenolide). Material and methods: The drug effect was assessed measuring the proliferation of lymphocytes using the 2,3-bis (2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino) carbonylJ-2H-tetrazolium hydroxide (XTT) technique. Changes on the cell cycle were analyzed on a FACSort flow cytometer The effect of millerenolide on the production of nitric oxide (NO) by macrophages was evaluated using the Griess reagent. Additionally, phagocytosis of latex particles and nitroblue tetrazolium (NBT) reduction by macrophages were evaluated microscopically. Results: Treatment of human peripheral blood mononuclear cells (PBMC) with millerenolide decreases the proliferation of lymphocytes, decreases the percentage of cells in S, and G2/Mphases, and increases the proportion of cells in GO/Gl phase. Treatment of macrophages with millerenolide, reduces the production of NO, the phagocytic capacity and the number of cells able to reduce NBT. Cytotoxic effects of the lactone on human PBMC were only observed when the concentration was increased to 6 fig/ml. Conclusions: Millerenolide could be considered as a potential therapeutic agent with immunosuppressiveproperties (RevMéd Chile 2007; 135: 64-72)
author	Dupuy L,Ornar A Murillo,Renato Bonilla V,José A
author_facet	Dupuy L,Ornar A Murillo,Renato Bonilla V,José A
author_sort	Dupuy L,Ornar A
title	Actividad supresora del millerenólido sobre células mononucleares de sangre periférica humana
title_short	Actividad supresora del millerenólido sobre células mononucleares de sangre periférica humana
title_full	Actividad supresora del millerenólido sobre células mononucleares de sangre periférica humana
title_fullStr	Actividad supresora del millerenólido sobre células mononucleares de sangre periférica humana
title_full_unstemmed	Actividad supresora del millerenólido sobre células mononucleares de sangre periférica humana
title_sort	actividad supresora del millerenólido sobre células mononucleares de sangre periférica humana
publisher	Sociedad Médica de Santiago
publishDate	2008
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872008000100008
work_keys_str_mv	AT dupuylornara actividadsupresoradelmillerenolidosobrecelulasmononuclearesdesangreperifericahumana AT murillorenato actividadsupresoradelmillerenolidosobrecelulasmononuclearesdesangreperifericahumana AT bonillavjosea actividadsupresoradelmillerenolidosobrecelulasmononuclearesdesangreperifericahumana
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Actividad supresora del millerenólido sobre células mononucleares de sangre periférica humana

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