Estudio de patrones de metilación génica en tumores del tubo digestivo

Background: The loss of tumor suppresor gene function damages the defensive mechanisms that protect the indemnity of genetic material. Promoter gene methylation is one of the inactivation mechanisms of suppressor genes. Aim: To study the methylation pattern of a group of genes in biopsy samples of g...

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Autores principales:	Roa S,Juan Carlos, García M,Patricia, Melo A,Angélica, Tapia E,Oscar, Villaseca H,Miguel, Araya O,Juan Carlos, Guzmán G,Pablo
Lenguaje:	Spanish / Castilian
Publicado:	Sociedad Médica de Santiago 2008
Materias:	Digestive system neoplasms Genes, supressor Methylation
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872008000400005
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spelling	oai:scielo:S0034-988720080004000052008-06-16Estudio de patrones de metilación génica en tumores del tubo digestivoRoa S,Juan CarlosGarcía M,PatriciaMelo A,AngélicaTapia E,OscarVillaseca H,MiguelAraya O,Juan CarlosGuzmán G,Pablo Digestive system neoplasms Genes, supressor Methylation Background: The loss of tumor suppresor gene function damages the defensive mechanisms that protect the indemnity of genetic material. Promoter gene methylation is one of the inactivation mechanisms of suppressor genes. Aim: To study the methylation pattern of a group of genes in biopsy samples of gastrointestinal tumors. Material and methods: Forty eight gastric, 25 gallbladder, 24 colon and 6 pancreas cancer biopsy samples were randomly selected. The methylation pattern of CDH1, FHIT, CDKN2A, APC and MLH1 genes, was studied using a specific polymerase chain reaction test for methylation. Demographic, morphological and follow up variables of patients bearing the tumors were also analyzed. Results: The general methylation frequency of CDH1, FHIT, CDKN2A, APC and MLH1 genes was 64.1, 56, 39.8, 18.1 and 34% respectively. In gastric cancer samples there was a correlation between APC gene methylation and well differentiated tumors; between CDH1 methylation and Lauren diffuse type and the presence of three or more metastasic lymph nodes; between FHIT, CDKN2A and CDH1 gene methylation and male gender. In ¡ess differentiated gallbladder tumors, the frequency of CDH1 methylation was higher. There was a tendency towards a lower survival in colon and gastric cancer when MLH1 (p =0.07) y CDKN2A (p= 0.06) were methylated, respectively. Conclusions: An abnormal methylation pattern was associated with morphological features in gastric and gallbladder cancer and with a tendency towards a lower survival in colon and gastric cancerinfo:eu-repo/semantics/openAccessSociedad Médica de SantiagoRevista médica de Chile v.136 n.4 20082008-04-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872008000400005es10.4067/S0034-98872008000400005
institution	Scielo Chile
collection	Scielo Chile
language	Spanish / Castilian
topic	Digestive system neoplasms Genes, supressor Methylation
spellingShingle	Digestive system neoplasms Genes, supressor Methylation Roa S,Juan Carlos García M,Patricia Melo A,Angélica Tapia E,Oscar Villaseca H,Miguel Araya O,Juan Carlos Guzmán G,Pablo Estudio de patrones de metilación génica en tumores del tubo digestivo
description	Background: The loss of tumor suppresor gene function damages the defensive mechanisms that protect the indemnity of genetic material. Promoter gene methylation is one of the inactivation mechanisms of suppressor genes. Aim: To study the methylation pattern of a group of genes in biopsy samples of gastrointestinal tumors. Material and methods: Forty eight gastric, 25 gallbladder, 24 colon and 6 pancreas cancer biopsy samples were randomly selected. The methylation pattern of CDH1, FHIT, CDKN2A, APC and MLH1 genes, was studied using a specific polymerase chain reaction test for methylation. Demographic, morphological and follow up variables of patients bearing the tumors were also analyzed. Results: The general methylation frequency of CDH1, FHIT, CDKN2A, APC and MLH1 genes was 64.1, 56, 39.8, 18.1 and 34% respectively. In gastric cancer samples there was a correlation between APC gene methylation and well differentiated tumors; between CDH1 methylation and Lauren diffuse type and the presence of three or more metastasic lymph nodes; between FHIT, CDKN2A and CDH1 gene methylation and male gender. In ¡ess differentiated gallbladder tumors, the frequency of CDH1 methylation was higher. There was a tendency towards a lower survival in colon and gastric cancer when MLH1 (p =0.07) y CDKN2A (p= 0.06) were methylated, respectively. Conclusions: An abnormal methylation pattern was associated with morphological features in gastric and gallbladder cancer and with a tendency towards a lower survival in colon and gastric cancer
author	Roa S,Juan Carlos García M,Patricia Melo A,Angélica Tapia E,Oscar Villaseca H,Miguel Araya O,Juan Carlos Guzmán G,Pablo
author_facet	Roa S,Juan Carlos García M,Patricia Melo A,Angélica Tapia E,Oscar Villaseca H,Miguel Araya O,Juan Carlos Guzmán G,Pablo
author_sort	Roa S,Juan Carlos
title	Estudio de patrones de metilación génica en tumores del tubo digestivo
title_short	Estudio de patrones de metilación génica en tumores del tubo digestivo
title_full	Estudio de patrones de metilación génica en tumores del tubo digestivo
title_fullStr	Estudio de patrones de metilación génica en tumores del tubo digestivo
title_full_unstemmed	Estudio de patrones de metilación génica en tumores del tubo digestivo
title_sort	estudio de patrones de metilación génica en tumores del tubo digestivo
publisher	Sociedad Médica de Santiago
publishDate	2008
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872008000400005
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Estudio de patrones de metilación génica en tumores del tubo digestivo

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