Caracterización molecular en aislados chilenos de Streptococcus agalactiae

Streptococcus agalactiae is the main causing organism of invasive infections such as sepsis and meningitis in the newborn. Aim: To perform a genotype characterization of Streptococcus agalactiae strains coming form invasive infections of newborns and colonized pregnant women. Material and methods: A...

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Autores principales:	Rojo,Patricia, Araya,Pamela, Martínez T,M Angélica, Hormazábal,Juan Carlos, Maldonado,Aurora, Fernández,Jorge
Lenguaje:	Spanish / Castilian
Publicado:	Sociedad Médica de Santiago 2008
Materias:	Clindamycin Drug resistance bacterial Erythromycin Streptococcus agalactiae
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872008000500009
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spelling	oai:scielo:S0034-988720080005000092008-07-30Caracterización molecular en aislados chilenos de Streptococcus agalactiaeRojo,PatriciaAraya,PamelaMartínez T,M AngélicaHormazábal,Juan CarlosMaldonado,AuroraFernández,Jorge Clindamycin Drug resistance bacterial Erythromycin Streptococcus agalactiae Streptococcus agalactiae is the main causing organism of invasive infections such as sepsis and meningitis in the newborn. Aim: To perform a genotype characterization of Streptococcus agalactiae strains coming form invasive infections of newborns and colonized pregnant women. Material and methods: A group of 58 strains not related epidemiologically isolated from colonized pregnant women and invasive infections in newborns, were studied. Pulsed field electrophoresis (PFGE) and polymerase chain reaction amplification of hylB and IS 1548 genes, as possible virulence markers, were performed. Results: Among the studied strains, 37 genetic subtypes were observed. There were nine groups of identical PFGE patterns. Three corresponded to serotype la and six to serotype III. An erythromycin and clindamycin resistant clone was identified in three colonized women and a newborn with sepsis, which were not epidemiologically related. The hylB gene was equally present in cases of neonatal meningitis or colonized pregnant women. Conclusions: There was a great degree of polymorphism among the studied strains. The ample presence of hylB gene and the absence of the insertion element IS1548 in the hylB gene in invasive and colonizing strains, indicates that both groups of strains are potentially pathogenicinfo:eu-repo/semantics/openAccessSociedad Médica de SantiagoRevista médica de Chile v.136 n.5 20082008-05-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872008000500009es10.4067/S0034-98872008000500009
institution	Scielo Chile
collection	Scielo Chile
language	Spanish / Castilian
topic	Clindamycin Drug resistance bacterial Erythromycin Streptococcus agalactiae
spellingShingle	Clindamycin Drug resistance bacterial Erythromycin Streptococcus agalactiae Rojo,Patricia Araya,Pamela Martínez T,M Angélica Hormazábal,Juan Carlos Maldonado,Aurora Fernández,Jorge Caracterización molecular en aislados chilenos de Streptococcus agalactiae
description	Streptococcus agalactiae is the main causing organism of invasive infections such as sepsis and meningitis in the newborn. Aim: To perform a genotype characterization of Streptococcus agalactiae strains coming form invasive infections of newborns and colonized pregnant women. Material and methods: A group of 58 strains not related epidemiologically isolated from colonized pregnant women and invasive infections in newborns, were studied. Pulsed field electrophoresis (PFGE) and polymerase chain reaction amplification of hylB and IS 1548 genes, as possible virulence markers, were performed. Results: Among the studied strains, 37 genetic subtypes were observed. There were nine groups of identical PFGE patterns. Three corresponded to serotype la and six to serotype III. An erythromycin and clindamycin resistant clone was identified in three colonized women and a newborn with sepsis, which were not epidemiologically related. The hylB gene was equally present in cases of neonatal meningitis or colonized pregnant women. Conclusions: There was a great degree of polymorphism among the studied strains. The ample presence of hylB gene and the absence of the insertion element IS1548 in the hylB gene in invasive and colonizing strains, indicates that both groups of strains are potentially pathogenic
author	Rojo,Patricia Araya,Pamela Martínez T,M Angélica Hormazábal,Juan Carlos Maldonado,Aurora Fernández,Jorge
author_facet	Rojo,Patricia Araya,Pamela Martínez T,M Angélica Hormazábal,Juan Carlos Maldonado,Aurora Fernández,Jorge
author_sort	Rojo,Patricia
title	Caracterización molecular en aislados chilenos de Streptococcus agalactiae
title_short	Caracterización molecular en aislados chilenos de Streptococcus agalactiae
title_full	Caracterización molecular en aislados chilenos de Streptococcus agalactiae
title_fullStr	Caracterización molecular en aislados chilenos de Streptococcus agalactiae
title_full_unstemmed	Caracterización molecular en aislados chilenos de Streptococcus agalactiae
title_sort	caracterización molecular en aislados chilenos de streptococcus agalactiae
publisher	Sociedad Médica de Santiago
publishDate	2008
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872008000500009
work_keys_str_mv	AT rojopatricia caracterizacionmolecularenaisladoschilenosdestreptococcusagalactiae AT arayapamela caracterizacionmolecularenaisladoschilenosdestreptococcusagalactiae AT martineztmangelica caracterizacionmolecularenaisladoschilenosdestreptococcusagalactiae AT hormazabaljuancarlos caracterizacionmolecularenaisladoschilenosdestreptococcusagalactiae AT maldonadoaurora caracterizacionmolecularenaisladoschilenosdestreptococcusagalactiae AT fernandezjorge caracterizacionmolecularenaisladoschilenosdestreptococcusagalactiae
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Caracterización molecular en aislados chilenos de Streptococcus agalactiae

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