Banco de tumores en Chile, su aporte a la investigación: resultados de un proyecto piloto

Background: Despite having the technical facilities and the knowledge, Chile did not have a tumor bank until recently. Aimn: To describe the results of the first three years of a tumor bank. Material and methods: All cases stored in a tumor bank from June 2004 tojune 2007 were included. Samples were...

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Autores principales:	Roa E,Iván, Artigas A,Carmen G
Lenguaje:	Spanish / Castilian
Publicado:	Sociedad Médica de Santiago 2008
Materias:	Breast neoplasms Cryopreservation Human tumour banking Tissue banks
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872008000600007
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spelling	oai:scielo:S0034-988720080006000072008-08-26Banco de tumores en Chile, su aporte a la investigación: resultados de un proyecto pilotoRoa E,IvánArtigas A,Carmen G Breast neoplasms Cryopreservation Human tumour banking Tissue banks Background: Despite having the technical facilities and the knowledge, Chile did not have a tumor bank until recently. Aimn: To describe the results of the first three years of a tumor bank. Material and methods: All cases stored in a tumor bank from June 2004 tojune 2007 were included. Samples were frozen in isopentane, afterwards in liquid nitrogen and finally transferred to freezers at -80°C. Quality controls with DNA and RNA extraction and immunohistochemistry, were per formed. Results: In the study period, 1239 cases were collected and 79% were malignant tumors. In 78% of cases, samples from the tumor and of normal neighaboring tissue, were stored. Twenty six percent of samples were from breast cáncer and 22% for digestive tumors. Immunohistochemical expression ofvimentin was measured in 30 cases and the expression of Ki67 an p53 in 20 cases. Thirteen of 15 breast cáncer samples had expression of estrogen receptors. In 30 cases, DNA and RNA extraction was carried out, amplifying B-globin and B-actin. Moreover RNA was extracted from 63 gastríc cáncer, 30 colon cáncer and gallbladder cáncer samples, for specific projects. Conclusions: The creation of a tumor bank is feasible, preserving samples ofhigh biológical qualityinfo:eu-repo/semantics/openAccessSociedad Médica de SantiagoRevista médica de Chile v.136 n.6 20082008-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872008000600007es10.4067/S0034-98872008000600007
institution	Scielo Chile
collection	Scielo Chile
language	Spanish / Castilian
topic	Breast neoplasms Cryopreservation Human tumour banking Tissue banks
spellingShingle	Breast neoplasms Cryopreservation Human tumour banking Tissue banks Roa E,Iván Artigas A,Carmen G Banco de tumores en Chile, su aporte a la investigación: resultados de un proyecto piloto
description	Background: Despite having the technical facilities and the knowledge, Chile did not have a tumor bank until recently. Aimn: To describe the results of the first three years of a tumor bank. Material and methods: All cases stored in a tumor bank from June 2004 tojune 2007 were included. Samples were frozen in isopentane, afterwards in liquid nitrogen and finally transferred to freezers at -80°C. Quality controls with DNA and RNA extraction and immunohistochemistry, were per formed. Results: In the study period, 1239 cases were collected and 79% were malignant tumors. In 78% of cases, samples from the tumor and of normal neighaboring tissue, were stored. Twenty six percent of samples were from breast cáncer and 22% for digestive tumors. Immunohistochemical expression ofvimentin was measured in 30 cases and the expression of Ki67 an p53 in 20 cases. Thirteen of 15 breast cáncer samples had expression of estrogen receptors. In 30 cases, DNA and RNA extraction was carried out, amplifying B-globin and B-actin. Moreover RNA was extracted from 63 gastríc cáncer, 30 colon cáncer and gallbladder cáncer samples, for specific projects. Conclusions: The creation of a tumor bank is feasible, preserving samples ofhigh biológical quality
author	Roa E,Iván Artigas A,Carmen G
author_facet	Roa E,Iván Artigas A,Carmen G
author_sort	Roa E,Iván
title	Banco de tumores en Chile, su aporte a la investigación: resultados de un proyecto piloto
title_short	Banco de tumores en Chile, su aporte a la investigación: resultados de un proyecto piloto
title_full	Banco de tumores en Chile, su aporte a la investigación: resultados de un proyecto piloto
title_fullStr	Banco de tumores en Chile, su aporte a la investigación: resultados de un proyecto piloto
title_full_unstemmed	Banco de tumores en Chile, su aporte a la investigación: resultados de un proyecto piloto
title_sort	banco de tumores en chile, su aporte a la investigación: resultados de un proyecto piloto
publisher	Sociedad Médica de Santiago
publishDate	2008
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872008000600007
work_keys_str_mv	AT roaeivan bancodetumoresenchilesuaportealainvestigacionresultadosdeunproyectopiloto AT artigasacarmeng bancodetumoresenchilesuaportealainvestigacionresultadosdeunproyectopiloto
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Banco de tumores en Chile, su aporte a la investigación: resultados de un proyecto piloto

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