La melatonina reduce la respuesta de cortisol al ACTH en humanos

Background: Melatonin receptors are widely distributed in human tissues but they have not been reported in human adrenal gland. Aim: To assess if the human adrenal gland expresses melatonin receptors and if melatonin affeets cortisol response to ACTH in dexamethasone suppressed volunteers. Material...

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Autores principales:	Campino,Carmen, Valenzuela,Francisco, Arteaga,Eugenio, Torres-Farfán,Claudia, Trucco,Cristián, Velasco,Alfredo, Guzmán,Sergio, Serón-Ferré,María
Lenguaje:	Spanish / Castilian
Publicado:	Sociedad Médica de Santiago 2008
Materias:	Adrenocorticotropic hormone Hydrocortisone Melatonin
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872008001100004
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spelling	oai:scielo:S0034-988720080011000042009-03-23La melatonina reduce la respuesta de cortisol al ACTH en humanosCampino,CarmenValenzuela,FranciscoArteaga,EugenioTorres-Farfán,ClaudiaTrucco,CristiánVelasco,AlfredoGuzmán,SergioSerón-Ferré,María Adrenocorticotropic hormone Hydrocortisone Melatonin Background: Melatonin receptors are widely distributed in human tissues but they have not been reported in human adrenal gland. Aim: To assess if the human adrenal gland expresses melatonin receptors and if melatonin affeets cortisol response to ACTH in dexamethasone suppressed volunteers. Material and methods: Adrenal glands were obtained from 4 patients undergoing unilateral nephrectomy-adrenalectomy for renal cáncer. Expression of mRNA MT1 and MT2 melatonin receptors was measured by Reverse Transcriptase Polymerase Chain Reaction (RT-PCR). The effect of melatonin on the response to intravenous (i.v.) ACTH was tested (randomized cross-over, double-blind, placebo-controlled tríal) in eight young healthy males pretreated with dexamethasone (1 mg) at 23:00 h. On the next day at 08:00 h, an i.v. Une was inserted, at 08:30 h, and after a blood sample, subjeets ingested 6 mg melatonin or placebo. At 09:00 h, 1-24 ACTH (Cortrosyn, 1µg/1.73 m² body surface área) was injected, drawing samples at 0, 15, 30, 45 and 60 minutes after. Melatonin, cortisol, cortisone, progesterone, aldosterone, DHEA-S, testosterone and prolactin were measured by immunoassay. Results: The four adrenal glands expressed only MT1 receptor mRNA. Melatonin ingestión reduced the cortisol response to ACTH from 14.6+1.45µg/dl at 60 min in the placebo group to 10.8+1.2µg/dl in the melatonin group (p <0.01 mixed model test). It did not affect other steroid hormone levels and abolished the morningphysiological decline of prolactin. Conclusions: The expression ofMTl melatonin receptor in the human adrenal, and the melatonin reduction of ACTH-stimulated cortisol production suggest a direct melatonin action on the adrenal gland .info:eu-repo/semantics/openAccessSociedad Médica de SantiagoRevista médica de Chile v.136 n.11 20082008-11-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872008001100004es10.4067/S0034-98872008001100004
institution	Scielo Chile
collection	Scielo Chile
language	Spanish / Castilian
topic	Adrenocorticotropic hormone Hydrocortisone Melatonin
spellingShingle	Adrenocorticotropic hormone Hydrocortisone Melatonin Campino,Carmen Valenzuela,Francisco Arteaga,Eugenio Torres-Farfán,Claudia Trucco,Cristián Velasco,Alfredo Guzmán,Sergio Serón-Ferré,María La melatonina reduce la respuesta de cortisol al ACTH en humanos
description	Background: Melatonin receptors are widely distributed in human tissues but they have not been reported in human adrenal gland. Aim: To assess if the human adrenal gland expresses melatonin receptors and if melatonin affeets cortisol response to ACTH in dexamethasone suppressed volunteers. Material and methods: Adrenal glands were obtained from 4 patients undergoing unilateral nephrectomy-adrenalectomy for renal cáncer. Expression of mRNA MT1 and MT2 melatonin receptors was measured by Reverse Transcriptase Polymerase Chain Reaction (RT-PCR). The effect of melatonin on the response to intravenous (i.v.) ACTH was tested (randomized cross-over, double-blind, placebo-controlled tríal) in eight young healthy males pretreated with dexamethasone (1 mg) at 23:00 h. On the next day at 08:00 h, an i.v. Une was inserted, at 08:30 h, and after a blood sample, subjeets ingested 6 mg melatonin or placebo. At 09:00 h, 1-24 ACTH (Cortrosyn, 1µg/1.73 m² body surface área) was injected, drawing samples at 0, 15, 30, 45 and 60 minutes after. Melatonin, cortisol, cortisone, progesterone, aldosterone, DHEA-S, testosterone and prolactin were measured by immunoassay. Results: The four adrenal glands expressed only MT1 receptor mRNA. Melatonin ingestión reduced the cortisol response to ACTH from 14.6+1.45µg/dl at 60 min in the placebo group to 10.8+1.2µg/dl in the melatonin group (p <0.01 mixed model test). It did not affect other steroid hormone levels and abolished the morningphysiological decline of prolactin. Conclusions: The expression ofMTl melatonin receptor in the human adrenal, and the melatonin reduction of ACTH-stimulated cortisol production suggest a direct melatonin action on the adrenal gland .
author	Campino,Carmen Valenzuela,Francisco Arteaga,Eugenio Torres-Farfán,Claudia Trucco,Cristián Velasco,Alfredo Guzmán,Sergio Serón-Ferré,María
author_facet	Campino,Carmen Valenzuela,Francisco Arteaga,Eugenio Torres-Farfán,Claudia Trucco,Cristián Velasco,Alfredo Guzmán,Sergio Serón-Ferré,María
author_sort	Campino,Carmen
title	La melatonina reduce la respuesta de cortisol al ACTH en humanos
title_short	La melatonina reduce la respuesta de cortisol al ACTH en humanos
title_full	La melatonina reduce la respuesta de cortisol al ACTH en humanos
title_fullStr	La melatonina reduce la respuesta de cortisol al ACTH en humanos
title_full_unstemmed	La melatonina reduce la respuesta de cortisol al ACTH en humanos
title_sort	la melatonina reduce la respuesta de cortisol al acth en humanos
publisher	Sociedad Médica de Santiago
publishDate	2008
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872008001100004
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La melatonina reduce la respuesta de cortisol al ACTH en humanos

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