Isla de patogenicidad de Vibrio parahaemolyticus en cepas chilenas clínicas y ambientales
Background: Most clinical isolates of Vibrio parahaemolyticus produce a major virulence factor known as the thermostable direct hemolysin (TDH). TDH is encoded by the tdh gene which is located in a genomic pathogenicity island (PAI). Most environmental isolates are described as tdh negative. Aim: To...
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Sociedad Médica de Santiago
2009
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oai:scielo:S0034-988720090002000042009-06-10Isla de patogenicidad de Vibrio parahaemolyticus en cepas chilenas clínicas y ambientalesNúñez,HaroldUlloa,María TeresaGuerra,FabiolaOsorio,Carlos G Genome, viral Hemolysis, Vibrio Vibrio parahaemolyticus Background: Most clinical isolates of Vibrio parahaemolyticus produce a major virulence factor known as the thermostable direct hemolysin (TDH). TDH is encoded by the tdh gene which is located in a genomic pathogenicity island (PAI). Most environmental isolates are described as tdh negative. Aim: To assess if environmental strains lack the full pathogenicity island or if only the tdh gene is deleted. Material and methods: Thirty eight clinical and 66 environmental strains of Vibrio parahaemolyticus were studied. PAI was characterized by polymerase chain reaction (PCR). The presence of tdhA and tdhS genes, was determined by Southern blot. Results: Fifty three environmental strains (80%) lacked a full PAI when compared with clinical strains. In environmental strains, Southern blot and sequence analysis showed that a genetic región of 80 kilobase pairs including genes from VPA1310 to VPA1396 was missing. Conclusions: These results highlight the genetic dynamism of Vibrio parahaemolyticus pathogenecity island región and suggest that new pathogenic strains could appear by horizontal transfer of the island between toxigenic and non-toxigenic strains.info:eu-repo/semantics/openAccessSociedad Médica de SantiagoRevista médica de Chile v.137 n.2 20092009-02-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872009000200004es10.4067/S0034-98872009000200004 |
institution |
Scielo Chile |
collection |
Scielo Chile |
language |
Spanish / Castilian |
topic |
Genome, viral Hemolysis, Vibrio Vibrio parahaemolyticus |
spellingShingle |
Genome, viral Hemolysis, Vibrio Vibrio parahaemolyticus Núñez,Harold Ulloa,María Teresa Guerra,Fabiola Osorio,Carlos G Isla de patogenicidad de Vibrio parahaemolyticus en cepas chilenas clínicas y ambientales |
description |
Background: Most clinical isolates of Vibrio parahaemolyticus produce a major virulence factor known as the thermostable direct hemolysin (TDH). TDH is encoded by the tdh gene which is located in a genomic pathogenicity island (PAI). Most environmental isolates are described as tdh negative. Aim: To assess if environmental strains lack the full pathogenicity island or if only the tdh gene is deleted. Material and methods: Thirty eight clinical and 66 environmental strains of Vibrio parahaemolyticus were studied. PAI was characterized by polymerase chain reaction (PCR). The presence of tdhA and tdhS genes, was determined by Southern blot. Results: Fifty three environmental strains (80%) lacked a full PAI when compared with clinical strains. In environmental strains, Southern blot and sequence analysis showed that a genetic región of 80 kilobase pairs including genes from VPA1310 to VPA1396 was missing. Conclusions: These results highlight the genetic dynamism of Vibrio parahaemolyticus pathogenecity island región and suggest that new pathogenic strains could appear by horizontal transfer of the island between toxigenic and non-toxigenic strains. |
author |
Núñez,Harold Ulloa,María Teresa Guerra,Fabiola Osorio,Carlos G |
author_facet |
Núñez,Harold Ulloa,María Teresa Guerra,Fabiola Osorio,Carlos G |
author_sort |
Núñez,Harold |
title |
Isla de patogenicidad de Vibrio parahaemolyticus en cepas chilenas clínicas y ambientales |
title_short |
Isla de patogenicidad de Vibrio parahaemolyticus en cepas chilenas clínicas y ambientales |
title_full |
Isla de patogenicidad de Vibrio parahaemolyticus en cepas chilenas clínicas y ambientales |
title_fullStr |
Isla de patogenicidad de Vibrio parahaemolyticus en cepas chilenas clínicas y ambientales |
title_full_unstemmed |
Isla de patogenicidad de Vibrio parahaemolyticus en cepas chilenas clínicas y ambientales |
title_sort |
isla de patogenicidad de vibrio parahaemolyticus en cepas chilenas clínicas y ambientales |
publisher |
Sociedad Médica de Santiago |
publishDate |
2009 |
url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872009000200004 |
work_keys_str_mv |
AT nunezharold isladepatogenicidaddevibrioparahaemolyticusencepaschilenasclinicasyambientales AT ulloamariateresa isladepatogenicidaddevibrioparahaemolyticusencepaschilenasclinicasyambientales AT guerrafabiola isladepatogenicidaddevibrioparahaemolyticusencepaschilenasclinicasyambientales AT osoriocarlosg isladepatogenicidaddevibrioparahaemolyticusencepaschilenasclinicasyambientales |
_version_ |
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