Isla de patogenicidad de Vibrio parahaemolyticus en cepas chilenas clínicas y ambientales

Background: Most clinical isolates of Vibrio parahaemolyticus produce a major virulence factor known as the thermostable direct hemolysin (TDH). TDH is encoded by the tdh gene which is located in a genomic pathogenicity island (PAI). Most environmental isolates are described as tdh negative. Aim: To...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Núñez,Harold, Ulloa,María Teresa, Guerra,Fabiola, Osorio,Carlos G
Lenguaje:Spanish / Castilian
Publicado: Sociedad Médica de Santiago 2009
Materias:
Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872009000200004
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:scielo:S0034-98872009000200004
record_format dspace
spelling oai:scielo:S0034-988720090002000042009-06-10Isla de patogenicidad de Vibrio parahaemolyticus en cepas chilenas clínicas y ambientalesNúñez,HaroldUlloa,María TeresaGuerra,FabiolaOsorio,Carlos G Genome, viral Hemolysis, Vibrio Vibrio parahaemolyticus Background: Most clinical isolates of Vibrio parahaemolyticus produce a major virulence factor known as the thermostable direct hemolysin (TDH). TDH is encoded by the tdh gene which is located in a genomic pathogenicity island (PAI). Most environmental isolates are described as tdh negative. Aim: To assess if environmental strains lack the full pathogenicity island or if only the tdh gene is deleted. Material and methods: Thirty eight clinical and 66 environmental strains of Vibrio parahaemolyticus were studied. PAI was characterized by polymerase chain reaction (PCR). The presence of tdhA and tdhS genes, was determined by Southern blot. Results: Fifty three environmental strains (80%) lacked a full PAI when compared with clinical strains. In environmental strains, Southern blot and sequence analysis showed that a genetic región of 80 kilobase pairs including genes from VPA1310 to VPA1396 was missing. Conclusions: These results highlight the genetic dynamism of Vibrio parahaemolyticus pathogenecity island región and suggest that new pathogenic strains could appear by horizontal transfer of the island between toxigenic and non-toxigenic strains.info:eu-repo/semantics/openAccessSociedad Médica de SantiagoRevista médica de Chile v.137 n.2 20092009-02-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872009000200004es10.4067/S0034-98872009000200004
institution Scielo Chile
collection Scielo Chile
language Spanish / Castilian
topic Genome, viral
Hemolysis, Vibrio
Vibrio parahaemolyticus
spellingShingle Genome, viral
Hemolysis, Vibrio
Vibrio parahaemolyticus
Núñez,Harold
Ulloa,María Teresa
Guerra,Fabiola
Osorio,Carlos G
Isla de patogenicidad de Vibrio parahaemolyticus en cepas chilenas clínicas y ambientales
description Background: Most clinical isolates of Vibrio parahaemolyticus produce a major virulence factor known as the thermostable direct hemolysin (TDH). TDH is encoded by the tdh gene which is located in a genomic pathogenicity island (PAI). Most environmental isolates are described as tdh negative. Aim: To assess if environmental strains lack the full pathogenicity island or if only the tdh gene is deleted. Material and methods: Thirty eight clinical and 66 environmental strains of Vibrio parahaemolyticus were studied. PAI was characterized by polymerase chain reaction (PCR). The presence of tdhA and tdhS genes, was determined by Southern blot. Results: Fifty three environmental strains (80%) lacked a full PAI when compared with clinical strains. In environmental strains, Southern blot and sequence analysis showed that a genetic región of 80 kilobase pairs including genes from VPA1310 to VPA1396 was missing. Conclusions: These results highlight the genetic dynamism of Vibrio parahaemolyticus pathogenecity island región and suggest that new pathogenic strains could appear by horizontal transfer of the island between toxigenic and non-toxigenic strains.
author Núñez,Harold
Ulloa,María Teresa
Guerra,Fabiola
Osorio,Carlos G
author_facet Núñez,Harold
Ulloa,María Teresa
Guerra,Fabiola
Osorio,Carlos G
author_sort Núñez,Harold
title Isla de patogenicidad de Vibrio parahaemolyticus en cepas chilenas clínicas y ambientales
title_short Isla de patogenicidad de Vibrio parahaemolyticus en cepas chilenas clínicas y ambientales
title_full Isla de patogenicidad de Vibrio parahaemolyticus en cepas chilenas clínicas y ambientales
title_fullStr Isla de patogenicidad de Vibrio parahaemolyticus en cepas chilenas clínicas y ambientales
title_full_unstemmed Isla de patogenicidad de Vibrio parahaemolyticus en cepas chilenas clínicas y ambientales
title_sort isla de patogenicidad de vibrio parahaemolyticus en cepas chilenas clínicas y ambientales
publisher Sociedad Médica de Santiago
publishDate 2009
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872009000200004
work_keys_str_mv AT nunezharold isladepatogenicidaddevibrioparahaemolyticusencepaschilenasclinicasyambientales
AT ulloamariateresa isladepatogenicidaddevibrioparahaemolyticusencepaschilenasclinicasyambientales
AT guerrafabiola isladepatogenicidaddevibrioparahaemolyticusencepaschilenasclinicasyambientales
AT osoriocarlosg isladepatogenicidaddevibrioparahaemolyticusencepaschilenasclinicasyambientales
_version_ 1718436426497916928