Isla de patogenicidad de Vibrio parahaemolyticus en cepas chilenas clínicas y ambientales

Background: Most clinical isolates of Vibrio parahaemolyticus produce a major virulence factor known as the thermostable direct hemolysin (TDH). TDH is encoded by the tdh gene which is located in a genomic pathogenicity island (PAI). Most environmental isolates are described as tdh negative. Aim: To...

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Autores principales:	Núñez,Harold, Ulloa,María Teresa, Guerra,Fabiola, Osorio,Carlos G
Lenguaje:	Spanish / Castilian
Publicado:	Sociedad Médica de Santiago 2009
Materias:	Genome, viral Hemolysis, Vibrio Vibrio parahaemolyticus
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872009000200004
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id	oai:scielo:S0034-98872009000200004
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spelling	oai:scielo:S0034-988720090002000042009-06-10Isla de patogenicidad de Vibrio parahaemolyticus en cepas chilenas clínicas y ambientalesNúñez,HaroldUlloa,María TeresaGuerra,FabiolaOsorio,Carlos G Genome, viral Hemolysis, Vibrio Vibrio parahaemolyticus Background: Most clinical isolates of Vibrio parahaemolyticus produce a major virulence factor known as the thermostable direct hemolysin (TDH). TDH is encoded by the tdh gene which is located in a genomic pathogenicity island (PAI). Most environmental isolates are described as tdh negative. Aim: To assess if environmental strains lack the full pathogenicity island or if only the tdh gene is deleted. Material and methods: Thirty eight clinical and 66 environmental strains of Vibrio parahaemolyticus were studied. PAI was characterized by polymerase chain reaction (PCR). The presence of tdhA and tdhS genes, was determined by Southern blot. Results: Fifty three environmental strains (80%) lacked a full PAI when compared with clinical strains. In environmental strains, Southern blot and sequence analysis showed that a genetic región of 80 kilobase pairs including genes from VPA1310 to VPA1396 was missing. Conclusions: These results highlight the genetic dynamism of Vibrio parahaemolyticus pathogenecity island región and suggest that new pathogenic strains could appear by horizontal transfer of the island between toxigenic and non-toxigenic strains.info:eu-repo/semantics/openAccessSociedad Médica de SantiagoRevista médica de Chile v.137 n.2 20092009-02-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872009000200004es10.4067/S0034-98872009000200004
institution	Scielo Chile
collection	Scielo Chile
language	Spanish / Castilian
topic	Genome, viral Hemolysis, Vibrio Vibrio parahaemolyticus
spellingShingle	Genome, viral Hemolysis, Vibrio Vibrio parahaemolyticus Núñez,Harold Ulloa,María Teresa Guerra,Fabiola Osorio,Carlos G Isla de patogenicidad de Vibrio parahaemolyticus en cepas chilenas clínicas y ambientales
description	Background: Most clinical isolates of Vibrio parahaemolyticus produce a major virulence factor known as the thermostable direct hemolysin (TDH). TDH is encoded by the tdh gene which is located in a genomic pathogenicity island (PAI). Most environmental isolates are described as tdh negative. Aim: To assess if environmental strains lack the full pathogenicity island or if only the tdh gene is deleted. Material and methods: Thirty eight clinical and 66 environmental strains of Vibrio parahaemolyticus were studied. PAI was characterized by polymerase chain reaction (PCR). The presence of tdhA and tdhS genes, was determined by Southern blot. Results: Fifty three environmental strains (80%) lacked a full PAI when compared with clinical strains. In environmental strains, Southern blot and sequence analysis showed that a genetic región of 80 kilobase pairs including genes from VPA1310 to VPA1396 was missing. Conclusions: These results highlight the genetic dynamism of Vibrio parahaemolyticus pathogenecity island región and suggest that new pathogenic strains could appear by horizontal transfer of the island between toxigenic and non-toxigenic strains.
author	Núñez,Harold Ulloa,María Teresa Guerra,Fabiola Osorio,Carlos G
author_facet	Núñez,Harold Ulloa,María Teresa Guerra,Fabiola Osorio,Carlos G
author_sort	Núñez,Harold
title	Isla de patogenicidad de Vibrio parahaemolyticus en cepas chilenas clínicas y ambientales
title_short	Isla de patogenicidad de Vibrio parahaemolyticus en cepas chilenas clínicas y ambientales
title_full	Isla de patogenicidad de Vibrio parahaemolyticus en cepas chilenas clínicas y ambientales
title_fullStr	Isla de patogenicidad de Vibrio parahaemolyticus en cepas chilenas clínicas y ambientales
title_full_unstemmed	Isla de patogenicidad de Vibrio parahaemolyticus en cepas chilenas clínicas y ambientales
title_sort	isla de patogenicidad de vibrio parahaemolyticus en cepas chilenas clínicas y ambientales
publisher	Sociedad Médica de Santiago
publishDate	2009
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872009000200004
work_keys_str_mv	AT nunezharold isladepatogenicidaddevibrioparahaemolyticusencepaschilenasclinicasyambientales AT ulloamariateresa isladepatogenicidaddevibrioparahaemolyticusencepaschilenasclinicasyambientales AT guerrafabiola isladepatogenicidaddevibrioparahaemolyticusencepaschilenasclinicasyambientales AT osoriocarlosg isladepatogenicidaddevibrioparahaemolyticusencepaschilenasclinicasyambientales
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Isla de patogenicidad de Vibrio parahaemolyticus en cepas chilenas clínicas y ambientales

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