Caracterización de la región variable de integrones clase 1 presentes en cepas nosocomiales de Klebsiella pneumoniae

Background: Integrons are responsible for the capture and dissemination of resistance genes in Gram-negative bacteria. Aim: To characterize the variable region within class 1 integrons in nosocomial strains of Klebsiella pneumoniae. Material and Methods: Twenty-nine Klebsiella pneumoniae strains iso...

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Autores principales:	GUZMÁN,MILITZA, ALONSO,GUILLERMINA
Lenguaje:	Spanish / Castilian
Publicado:	Sociedad Médica de Santiago 2010
Materias:	Bacterial infections Integrons Klebsiella pneumoniae
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872010000300010
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spelling	oai:scielo:S0034-988720100003000102010-05-19Caracterización de la región variable de integrones clase 1 presentes en cepas nosocomiales de Klebsiella pneumoniaeGUZMÁN,MILITZAALONSO,GUILLERMINA Bacterial infections Integrons Klebsiella pneumoniae Background: Integrons are responsible for the capture and dissemination of resistance genes in Gram-negative bacteria. Aim: To characterize the variable region within class 1 integrons in nosocomial strains of Klebsiella pneumoniae. Material and Methods: Twenty-nine Klebsiella pneumoniae strains isolated from hospitalized patients were analyzed. The variable region of class 1 integrons was characterized by polymerase chain reaction and direct sequencing. Genetic localization of class 1 integrons was determined by bacterial conjugation. Results: Ten strains contained class 1 integrons, with sizes ranging from 750 to 2000 base pairs. One integrant element was present in nine strains and two elements in one single strain. Integrons were associated to plasmids. Cassettes aadA, aac(6)-Iq, orfD, dfrA]7, aadA5 and aadB were found. Conclusions: The presence of class 1 integrons may play an important role in the dissemination of hospital resistance against amino glycosides.info:eu-repo/semantics/openAccessSociedad Médica de SantiagoRevista médica de Chile v.138 n.3 20102010-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872010000300010es10.4067/S0034-98872010000300010
institution	Scielo Chile
collection	Scielo Chile
language	Spanish / Castilian
topic	Bacterial infections Integrons Klebsiella pneumoniae
spellingShingle	Bacterial infections Integrons Klebsiella pneumoniae GUZMÁN,MILITZA ALONSO,GUILLERMINA Caracterización de la región variable de integrones clase 1 presentes en cepas nosocomiales de Klebsiella pneumoniae
description	Background: Integrons are responsible for the capture and dissemination of resistance genes in Gram-negative bacteria. Aim: To characterize the variable region within class 1 integrons in nosocomial strains of Klebsiella pneumoniae. Material and Methods: Twenty-nine Klebsiella pneumoniae strains isolated from hospitalized patients were analyzed. The variable region of class 1 integrons was characterized by polymerase chain reaction and direct sequencing. Genetic localization of class 1 integrons was determined by bacterial conjugation. Results: Ten strains contained class 1 integrons, with sizes ranging from 750 to 2000 base pairs. One integrant element was present in nine strains and two elements in one single strain. Integrons were associated to plasmids. Cassettes aadA, aac(6)-Iq, orfD, dfrA]7, aadA5 and aadB were found. Conclusions: The presence of class 1 integrons may play an important role in the dissemination of hospital resistance against amino glycosides.
author	GUZMÁN,MILITZA ALONSO,GUILLERMINA
author_facet	GUZMÁN,MILITZA ALONSO,GUILLERMINA
author_sort	GUZMÁN,MILITZA
title	Caracterización de la región variable de integrones clase 1 presentes en cepas nosocomiales de Klebsiella pneumoniae
title_short	Caracterización de la región variable de integrones clase 1 presentes en cepas nosocomiales de Klebsiella pneumoniae
title_full	Caracterización de la región variable de integrones clase 1 presentes en cepas nosocomiales de Klebsiella pneumoniae
title_fullStr	Caracterización de la región variable de integrones clase 1 presentes en cepas nosocomiales de Klebsiella pneumoniae
title_full_unstemmed	Caracterización de la región variable de integrones clase 1 presentes en cepas nosocomiales de Klebsiella pneumoniae
title_sort	caracterización de la región variable de integrones clase 1 presentes en cepas nosocomiales de klebsiella pneumoniae
publisher	Sociedad Médica de Santiago
publishDate	2010
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872010000300010
work_keys_str_mv	AT guzmanmilitza caracterizaciondelaregionvariabledeintegronesclase1presentesencepasnosocomialesdeklebsiellapneumoniae AT alonsoguillermina caracterizaciondelaregionvariabledeintegronesclase1presentesencepasnosocomialesdeklebsiellapneumoniae
_version_	1718436494593490944

Caracterización de la región variable de integrones clase 1 presentes en cepas nosocomiales de Klebsiella pneumoniae

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