Comparación de los criterios de Nugent y Spiegel para el diagnóstico de vaginosis bacteriana y análisis de los resultados discordantes por el método de Ison y Hay

Background: Vaginal infection is the commonest cause of genital symptoms and has obstetric and gynecological implications. Aim: To compare the Nugent and Spiegel methods for the diagnosis of bacterial vaginosis (BV) and to analyze discordant specimens using Ison and Hay (Ison/Hay) criteria. Material...

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Autores principales:	MARTÍNEZ,MARÍA ANGÉLICA, OVALLE,ALFREDO, GAETE,ANA MARÍA, LILL0,EDUARDO, DE LA FUENTE,FELIPE, ARANEDA,FABIÁN, VILLASECA,RODRIGO, SALINAS,HUGO
Lenguaje:	Spanish / Castilian
Publicado:	Sociedad Médica de Santiago 2011
Materias:	Gram's stain Staining and labeling Vaginosis bacterial
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872011000100009
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spelling	oai:scielo:S0034-988720110001000092011-04-11Comparación de los criterios de Nugent y Spiegel para el diagnóstico de vaginosis bacteriana y análisis de los resultados discordantes por el método de Ison y HayMARTÍNEZ,MARÍA ANGÉLICAOVALLE,ALFREDOGAETE,ANA MARÍALILL0,EDUARDODE LA FUENTE,FELIPEARANEDA,FABIÁNVILLASECA,RODRIGOSALINAS,HUGO Gram's stain Staining and labeling Vaginosis bacterial Background: Vaginal infection is the commonest cause of genital symptoms and has obstetric and gynecological implications. Aim: To compare the Nugent and Spiegel methods for the diagnosis of bacterial vaginosis (BV) and to analyze discordant specimens using Ison and Hay (Ison/Hay) criteria. Material and Methods: After discardingcases with Candidiasis, deficientspecimens or those lacking bacteria, a total of348 Gram-stained smears vaginal specimens receivedfor the diagnosis of BV, were analyzed. Results: Vaginal microbiota was classified as normal in 203 and 237 samples (58 and 68% of samples), accordingto Nugent and Spiegel criteria, respectively One hundred andfive (30%) and 111 samples (32%), were classified as VB accordingto Nugent and Spiegel criteria, respectively. Both criteria were concordant in 308 samples (88.5%). The 40 (11.5%) discordant specimens were classified as intermedíate microflora by the Nugent system and as normal or BV by Spiegel. Among these, the Ison/Hay procedure identified four categories of microbiota. Ten (25%) specimens were classified as grade II microbiota, confirming their categorization by Nugent as intermedíate microbiota, six (15%) were classified in the III category, confirming the diagnosis ofBV by Spiegel, 13 (32.5%) corresponded to the category III, that does not exist in the Nugent and Spiegel categorization systems. Finally, 11 specimens could not be assigned to one category due to microscopic limitations to distinguish bacterial morphotypes. Conclusions: The systems proposed by Spiegel, Nugent and Ison I Hay are comparable for the diagnosis of BV. However, we recommend the use of Ison/ Hay procedure to evalúate vaginal microbiota, due to its wider range of categories, allowing a better discrimination ofthe vaginal microbiota.info:eu-repo/semantics/openAccessSociedad Médica de SantiagoRevista médica de Chile v.139 n.1 20112011-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872011000100009es10.4067/S0034-98872011000100009
institution	Scielo Chile
collection	Scielo Chile
language	Spanish / Castilian
topic	Gram's stain Staining and labeling Vaginosis bacterial
spellingShingle	Gram's stain Staining and labeling Vaginosis bacterial MARTÍNEZ,MARÍA ANGÉLICA OVALLE,ALFREDO GAETE,ANA MARÍA LILL0,EDUARDO DE LA FUENTE,FELIPE ARANEDA,FABIÁN VILLASECA,RODRIGO SALINAS,HUGO Comparación de los criterios de Nugent y Spiegel para el diagnóstico de vaginosis bacteriana y análisis de los resultados discordantes por el método de Ison y Hay
description	Background: Vaginal infection is the commonest cause of genital symptoms and has obstetric and gynecological implications. Aim: To compare the Nugent and Spiegel methods for the diagnosis of bacterial vaginosis (BV) and to analyze discordant specimens using Ison and Hay (Ison/Hay) criteria. Material and Methods: After discardingcases with Candidiasis, deficientspecimens or those lacking bacteria, a total of348 Gram-stained smears vaginal specimens receivedfor the diagnosis of BV, were analyzed. Results: Vaginal microbiota was classified as normal in 203 and 237 samples (58 and 68% of samples), accordingto Nugent and Spiegel criteria, respectively One hundred andfive (30%) and 111 samples (32%), were classified as VB accordingto Nugent and Spiegel criteria, respectively. Both criteria were concordant in 308 samples (88.5%). The 40 (11.5%) discordant specimens were classified as intermedíate microflora by the Nugent system and as normal or BV by Spiegel. Among these, the Ison/Hay procedure identified four categories of microbiota. Ten (25%) specimens were classified as grade II microbiota, confirming their categorization by Nugent as intermedíate microbiota, six (15%) were classified in the III category, confirming the diagnosis ofBV by Spiegel, 13 (32.5%) corresponded to the category III, that does not exist in the Nugent and Spiegel categorization systems. Finally, 11 specimens could not be assigned to one category due to microscopic limitations to distinguish bacterial morphotypes. Conclusions: The systems proposed by Spiegel, Nugent and Ison I Hay are comparable for the diagnosis of BV. However, we recommend the use of Ison/ Hay procedure to evalúate vaginal microbiota, due to its wider range of categories, allowing a better discrimination ofthe vaginal microbiota.
author	MARTÍNEZ,MARÍA ANGÉLICA OVALLE,ALFREDO GAETE,ANA MARÍA LILL0,EDUARDO DE LA FUENTE,FELIPE ARANEDA,FABIÁN VILLASECA,RODRIGO SALINAS,HUGO
author_facet	MARTÍNEZ,MARÍA ANGÉLICA OVALLE,ALFREDO GAETE,ANA MARÍA LILL0,EDUARDO DE LA FUENTE,FELIPE ARANEDA,FABIÁN VILLASECA,RODRIGO SALINAS,HUGO
author_sort	MARTÍNEZ,MARÍA ANGÉLICA
title	Comparación de los criterios de Nugent y Spiegel para el diagnóstico de vaginosis bacteriana y análisis de los resultados discordantes por el método de Ison y Hay
title_short	Comparación de los criterios de Nugent y Spiegel para el diagnóstico de vaginosis bacteriana y análisis de los resultados discordantes por el método de Ison y Hay
title_full	Comparación de los criterios de Nugent y Spiegel para el diagnóstico de vaginosis bacteriana y análisis de los resultados discordantes por el método de Ison y Hay
title_fullStr	Comparación de los criterios de Nugent y Spiegel para el diagnóstico de vaginosis bacteriana y análisis de los resultados discordantes por el método de Ison y Hay
title_full_unstemmed	Comparación de los criterios de Nugent y Spiegel para el diagnóstico de vaginosis bacteriana y análisis de los resultados discordantes por el método de Ison y Hay
title_sort	comparación de los criterios de nugent y spiegel para el diagnóstico de vaginosis bacteriana y análisis de los resultados discordantes por el método de ison y hay
publisher	Sociedad Médica de Santiago
publishDate	2011
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872011000100009
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Comparación de los criterios de Nugent y Spiegel para el diagnóstico de vaginosis bacteriana y análisis de los resultados discordantes por el método de Ison y Hay

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