Detección de ADN de parvovirus B19 en donantes de sangre de tres hospitales en Santiago, Chile
Background: Parvovirus B19 (B19) is associated with a wide range of diseases in humans, whose severity depends on the immunological and hematological status of the host. It is transmitted mainly through the airway but also by transfusions. Aim: To determine the B19 DNA carrier frequency in a populat...
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| Lenguaje: | Spanish / Castilian |
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Sociedad Médica de Santiago
2011
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| Acceso en línea: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872011000200001 |
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oai:scielo:S0034-988720110002000012011-08-01Detección de ADN de parvovirus B19 en donantes de sangre de tres hospitales en Santiago, ChileLÉVICAN,JORGETORRES,MAURICIOGAGGERO,NICOLÁSCORVALÁN,ROSAGAGGERO,ALDO Blood banks Genome, human Parvovirus B19, human Background: Parvovirus B19 (B19) is associated with a wide range of diseases in humans, whose severity depends on the immunological and hematological status of the host. It is transmitted mainly through the airway but also by transfusions. Aim: To determine the B19 DNA carrier frequency in a population of volunteer blood donors from three hospitals blood banks in Santiago, Chile, and to determine the viral load in DNA positive cases. Material and Methods: A total of477 serum samples were analyzed. The screening of B19 DNA was carried out by nested polymerase chain reaction (PCR) directed to the non-structural region of the virus (NS1). The viral load in positives cases was quantified by NS1 Real Time PCR. Results: Parvovirus B19 was detected in four samples, rendering a frequency of 1:119. The viral loads ranged from less than 2000 to 5,626 x 10(5) genome equivalents/ml. Conclusions: Parvovirus B19 was present in four of 477 blood bank blood donors from three hospitals in Santiago.info:eu-repo/semantics/openAccessSociedad Médica de SantiagoRevista médica de Chile v.139 n.2 20112011-02-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872011000200001es10.4067/S0034-98872011000200001 |
| institution |
Scielo Chile |
| collection |
Scielo Chile |
| language |
Spanish / Castilian |
| topic |
Blood banks Genome, human Parvovirus B19, human |
| spellingShingle |
Blood banks Genome, human Parvovirus B19, human LÉVICAN,JORGE TORRES,MAURICIO GAGGERO,NICOLÁS CORVALÁN,ROSA GAGGERO,ALDO Detección de ADN de parvovirus B19 en donantes de sangre de tres hospitales en Santiago, Chile |
| description |
Background: Parvovirus B19 (B19) is associated with a wide range of diseases in humans, whose severity depends on the immunological and hematological status of the host. It is transmitted mainly through the airway but also by transfusions. Aim: To determine the B19 DNA carrier frequency in a population of volunteer blood donors from three hospitals blood banks in Santiago, Chile, and to determine the viral load in DNA positive cases. Material and Methods: A total of477 serum samples were analyzed. The screening of B19 DNA was carried out by nested polymerase chain reaction (PCR) directed to the non-structural region of the virus (NS1). The viral load in positives cases was quantified by NS1 Real Time PCR. Results: Parvovirus B19 was detected in four samples, rendering a frequency of 1:119. The viral loads ranged from less than 2000 to 5,626 x 10(5) genome equivalents/ml. Conclusions: Parvovirus B19 was present in four of 477 blood bank blood donors from three hospitals in Santiago. |
| author |
LÉVICAN,JORGE TORRES,MAURICIO GAGGERO,NICOLÁS CORVALÁN,ROSA GAGGERO,ALDO |
| author_facet |
LÉVICAN,JORGE TORRES,MAURICIO GAGGERO,NICOLÁS CORVALÁN,ROSA GAGGERO,ALDO |
| author_sort |
LÉVICAN,JORGE |
| title |
Detección de ADN de parvovirus B19 en donantes de sangre de tres hospitales en Santiago, Chile |
| title_short |
Detección de ADN de parvovirus B19 en donantes de sangre de tres hospitales en Santiago, Chile |
| title_full |
Detección de ADN de parvovirus B19 en donantes de sangre de tres hospitales en Santiago, Chile |
| title_fullStr |
Detección de ADN de parvovirus B19 en donantes de sangre de tres hospitales en Santiago, Chile |
| title_full_unstemmed |
Detección de ADN de parvovirus B19 en donantes de sangre de tres hospitales en Santiago, Chile |
| title_sort |
detección de adn de parvovirus b19 en donantes de sangre de tres hospitales en santiago, chile |
| publisher |
Sociedad Médica de Santiago |
| publishDate |
2011 |
| url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872011000200001 |
| work_keys_str_mv |
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| _version_ |
1718436550724812800 |