Detección de mutaciones asociadas a cepas multidrogo resistente de Mycobacterium tuberculosis en Chile

Background: The incidence of acquired resistance to antituberculous drugs of Mycobacterium tuberculosis in Chile is approximately 23%. Aim: To analyze the mutations associated with drug resistance in drug resistant strains of Mycobacterium tuberculosis. Material and Methods: In 28 drug resistant Myc...

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Autores principales:	Araya,Pamela, Velasco,Maritza, Tognarelli,Javier, Arias,Fabiola, Leiva,Tamara, Sccapatticio,Angélica, Alviz,Pablo, Fernández,Jorge
Lenguaje:	Spanish / Castilian
Publicado:	Sociedad Médica de Santiago 2011
Materias:	Drug resistance microbial Mutation Mycobacterium tuberculosis
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872011000400008
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spelling	oai:scielo:S0034-988720110004000082011-08-25Detección de mutaciones asociadas a cepas multidrogo resistente de Mycobacterium tuberculosis en ChileAraya,PamelaVelasco,MaritzaTognarelli,JavierArias,FabiolaLeiva,TamaraSccapatticio,AngélicaAlviz,PabloFernández,Jorge Drug resistance microbial Mutation Mycobacterium tuberculosis Background: The incidence of acquired resistance to antituberculous drugs of Mycobacterium tuberculosis in Chile is approximately 23%. Aim: To analyze the mutations associated with drug resistance in drug resistant strains of Mycobacterium tuberculosis. Material and Methods: In 28 drug resistant Mycobacterium tuberculosis strains isolated in Chile, genes leading to drug resistance were studied. DNA was amplifed by polymerase chain reaction (PCR) and sequencing was carried out using the ABI PRISM big dye terminator cycle sequencing ready reaction kit. Results: In rifampicin-resistant strains, the mutations in rpoβ gene were in the codons S531W/L (56%), D516Y (16%) and D516V (16%). The predominant mutation in katG gene was in the codon S315L (73%) in isoniazid-resistant strains. The mutation S95T was found in the 71% of ciprofoxacin resistant strains. Only one ethambutol resistant strain had the M306I mutation. Three unreported mutations in katG were identifed. Conclusions: Drug resistance associated mutations of Mycobacterium tuberculosis isolated in Chile were similar to those reported abroad.info:eu-repo/semantics/openAccessSociedad Médica de SantiagoRevista médica de Chile v.139 n.4 20112011-04-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872011000400008es10.4067/S0034-98872011000400008
institution	Scielo Chile
collection	Scielo Chile
language	Spanish / Castilian
topic	Drug resistance microbial Mutation Mycobacterium tuberculosis
spellingShingle	Drug resistance microbial Mutation Mycobacterium tuberculosis Araya,Pamela Velasco,Maritza Tognarelli,Javier Arias,Fabiola Leiva,Tamara Sccapatticio,Angélica Alviz,Pablo Fernández,Jorge Detección de mutaciones asociadas a cepas multidrogo resistente de Mycobacterium tuberculosis en Chile
description	Background: The incidence of acquired resistance to antituberculous drugs of Mycobacterium tuberculosis in Chile is approximately 23%. Aim: To analyze the mutations associated with drug resistance in drug resistant strains of Mycobacterium tuberculosis. Material and Methods: In 28 drug resistant Mycobacterium tuberculosis strains isolated in Chile, genes leading to drug resistance were studied. DNA was amplifed by polymerase chain reaction (PCR) and sequencing was carried out using the ABI PRISM big dye terminator cycle sequencing ready reaction kit. Results: In rifampicin-resistant strains, the mutations in rpoβ gene were in the codons S531W/L (56%), D516Y (16%) and D516V (16%). The predominant mutation in katG gene was in the codon S315L (73%) in isoniazid-resistant strains. The mutation S95T was found in the 71% of ciprofoxacin resistant strains. Only one ethambutol resistant strain had the M306I mutation. Three unreported mutations in katG were identifed. Conclusions: Drug resistance associated mutations of Mycobacterium tuberculosis isolated in Chile were similar to those reported abroad.
author	Araya,Pamela Velasco,Maritza Tognarelli,Javier Arias,Fabiola Leiva,Tamara Sccapatticio,Angélica Alviz,Pablo Fernández,Jorge
author_facet	Araya,Pamela Velasco,Maritza Tognarelli,Javier Arias,Fabiola Leiva,Tamara Sccapatticio,Angélica Alviz,Pablo Fernández,Jorge
author_sort	Araya,Pamela
title	Detección de mutaciones asociadas a cepas multidrogo resistente de Mycobacterium tuberculosis en Chile
title_short	Detección de mutaciones asociadas a cepas multidrogo resistente de Mycobacterium tuberculosis en Chile
title_full	Detección de mutaciones asociadas a cepas multidrogo resistente de Mycobacterium tuberculosis en Chile
title_fullStr	Detección de mutaciones asociadas a cepas multidrogo resistente de Mycobacterium tuberculosis en Chile
title_full_unstemmed	Detección de mutaciones asociadas a cepas multidrogo resistente de Mycobacterium tuberculosis en Chile
title_sort	detección de mutaciones asociadas a cepas multidrogo resistente de mycobacterium tuberculosis en chile
publisher	Sociedad Médica de Santiago
publishDate	2011
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872011000400008
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Detección de mutaciones asociadas a cepas multidrogo resistente de Mycobacterium tuberculosis en Chile

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