Inicio del brote de Influenza A (H1N1) pandémica en Chile: caracterización genética de los primeros casos detectados

Background: Following the announcement of the Influenza A(H1N1) pandemic by the World Health Organization in April 2009, a surveillance program was carried out in Chile to detect the introduction of the virus in the country and to monitor its propagation and impact. Aim: To describe the onset of the...

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Autores principales:	FASCE,RODRIGO A, TOGNARELLI,JAVIER, MORA,JUDITH, TORRES,GRACIELA, ANDRADE,WINSTON, BUSTOS,PATRICIA, ARAYA,PAMELA, BALANDA,MONSERRAT, AGUAYO,CAROLINA, SEIN,JORGE, RAMÍREZ,EUGENIO, FERNÁNDEZ,JORGE
Lenguaje:	Spanish / Castilian
Publicado:	Sociedad Médica de Santiago 2011
Materias:	Influenza A virus H1N1 subtype Pandemics
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872011000700002
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spelling	oai:scielo:S0034-988720110007000022011-09-16Inicio del brote de Influenza A (H1N1) pandémica en Chile: caracterización genética de los primeros casos detectadosFASCE,RODRIGO ATOGNARELLI,JAVIERMORA,JUDITHTORRES,GRACIELAANDRADE,WINSTONBUSTOS,PATRICIAARAYA,PAMELABALANDA,MONSERRATAGUAYO,CAROLINASEIN,JORGERAMÍREZ,EUGENIOFERNÁNDEZ,JORGE Influenza A virus H1N1 subtype Pandemics Background: Following the announcement of the Influenza A(H1N1) pandemic by the World Health Organization in April 2009, a surveillance program was carried out in Chile to detect the introduction of the virus in the country and to monitor its propagation and impact. Aim: To describe the onset of the outbreak and the genetic characterization of the pandemic H1N1 influenza virus in the first detected cases in Chile. Material and Methods: Analysis of18 clinical samples coming from suspicious patients, received in a National Reference Laboratory. RNA reverse transcription and real time influenza gene DNA amplification was carried out in a 7500 Fast and Step One Real Time PCR Systems of Applied Biosystems and MxPro-Mx3000P thermocycler from Stratagene. Super Script III Platinum One-Step Quantitative RT-PCR was used. Results: The virus was first detected in three persons returning from the Dominican Republic via Panamá and a child from the east zone of Santiago. Genetic characterization of the virus showed that the child was infected by a different variant of the pandemic virus than the three persons returning from the Caribbean. Conclusions: The onset of the Influenza outbreak in Chile apparently carne from two different epidemiological groups. The spread of the virus detected in the voyagers was limited immediately However the virus of the fourth case was found in different regions of Chile.info:eu-repo/semantics/openAccessSociedad Médica de SantiagoRevista médica de Chile v.139 n.7 20112011-07-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872011000700002es10.4067/S0034-98872011000700002
institution	Scielo Chile
collection	Scielo Chile
language	Spanish / Castilian
topic	Influenza A virus H1N1 subtype Pandemics
spellingShingle	Influenza A virus H1N1 subtype Pandemics FASCE,RODRIGO A TOGNARELLI,JAVIER MORA,JUDITH TORRES,GRACIELA ANDRADE,WINSTON BUSTOS,PATRICIA ARAYA,PAMELA BALANDA,MONSERRAT AGUAYO,CAROLINA SEIN,JORGE RAMÍREZ,EUGENIO FERNÁNDEZ,JORGE Inicio del brote de Influenza A (H1N1) pandémica en Chile: caracterización genética de los primeros casos detectados
description	Background: Following the announcement of the Influenza A(H1N1) pandemic by the World Health Organization in April 2009, a surveillance program was carried out in Chile to detect the introduction of the virus in the country and to monitor its propagation and impact. Aim: To describe the onset of the outbreak and the genetic characterization of the pandemic H1N1 influenza virus in the first detected cases in Chile. Material and Methods: Analysis of18 clinical samples coming from suspicious patients, received in a National Reference Laboratory. RNA reverse transcription and real time influenza gene DNA amplification was carried out in a 7500 Fast and Step One Real Time PCR Systems of Applied Biosystems and MxPro-Mx3000P thermocycler from Stratagene. Super Script III Platinum One-Step Quantitative RT-PCR was used. Results: The virus was first detected in three persons returning from the Dominican Republic via Panamá and a child from the east zone of Santiago. Genetic characterization of the virus showed that the child was infected by a different variant of the pandemic virus than the three persons returning from the Caribbean. Conclusions: The onset of the Influenza outbreak in Chile apparently carne from two different epidemiological groups. The spread of the virus detected in the voyagers was limited immediately However the virus of the fourth case was found in different regions of Chile.
author	FASCE,RODRIGO A TOGNARELLI,JAVIER MORA,JUDITH TORRES,GRACIELA ANDRADE,WINSTON BUSTOS,PATRICIA ARAYA,PAMELA BALANDA,MONSERRAT AGUAYO,CAROLINA SEIN,JORGE RAMÍREZ,EUGENIO FERNÁNDEZ,JORGE
author_facet	FASCE,RODRIGO A TOGNARELLI,JAVIER MORA,JUDITH TORRES,GRACIELA ANDRADE,WINSTON BUSTOS,PATRICIA ARAYA,PAMELA BALANDA,MONSERRAT AGUAYO,CAROLINA SEIN,JORGE RAMÍREZ,EUGENIO FERNÁNDEZ,JORGE
author_sort	FASCE,RODRIGO A
title	Inicio del brote de Influenza A (H1N1) pandémica en Chile: caracterización genética de los primeros casos detectados
title_short	Inicio del brote de Influenza A (H1N1) pandémica en Chile: caracterización genética de los primeros casos detectados
title_full	Inicio del brote de Influenza A (H1N1) pandémica en Chile: caracterización genética de los primeros casos detectados
title_fullStr	Inicio del brote de Influenza A (H1N1) pandémica en Chile: caracterización genética de los primeros casos detectados
title_full_unstemmed	Inicio del brote de Influenza A (H1N1) pandémica en Chile: caracterización genética de los primeros casos detectados
title_sort	inicio del brote de influenza a (h1n1) pandémica en chile: caracterización genética de los primeros casos detectados
publisher	Sociedad Médica de Santiago
publishDate	2011
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872011000700002
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Inicio del brote de Influenza A (H1N1) pandémica en Chile: caracterización genética de los primeros casos detectados

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