Efecto in vitro de los terpenos lupeol y casearina G sobre células sanguíneas y tumorales

Background: The rainforest is an important source of natural compounds with therapeutic properties. Although there are many anti-inflammatory and antineoplastic drugs available to the clinician, there is an ongoing need for new therapeutic drugs with fewer serious adverse effects. Aim: To evaluate t...

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Autores principales:	Dupuy L,Omar A, Bonilla V,José A, Murillo,Renato, Taylor,Peter, Abad,María Jesús, González,Lorena, Juliao A,Johanna
Lenguaje:	Spanish / Castilian
Publicado:	Sociedad Médica de Santiago 2013
Materias:	Antineoplastic agents phytogenic Diterpenes clerodane Pentacyclic triterpenes
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872013000900007
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spelling	oai:scielo:S0034-988720130009000072014-09-02Efecto in vitro de los terpenos lupeol y casearina G sobre células sanguíneas y tumoralesDupuy L,Omar ABonilla V,José AMurillo,RenatoTaylor,PeterAbad,María JesúsGonzález,LorenaJuliao A,Johanna Antineoplastic agents phytogenic Diterpenes clerodane Pentacyclic triterpenes Background: The rainforest is an important source of natural compounds with therapeutic properties. Although there are many anti-inflammatory and antineoplastic drugs available to the clinician, there is an ongoing need for new therapeutic drugs with fewer serious adverse effects. Aim: To evaluate the in vitro cytotoxic effects of lupeol and casearin G on tumor cells, on phagocytic activity and nitric oxide (NO) production by blood mononuclear cells. Material and Methods: The cytotoxic effect of these compounds on cell lines MCF-7 (human breast adenocarcinoma) and PC-3 (human prostate cancer) was measured by a colorimetric assay (MTS/PMS) and the sulphorhodamine B assay. Peripheral blood mononuclear cells were obtained from eight healthy volunteers. The effect of these compounds on nitric oxide (NO) production was measured using the Griess reaction. Their effect on phagocytic activity of PBMC was also evaluated. Results: Lupeol (≥ 2 mM) resulted in a reduction of both the phagocytic index and the percentage of phagocytic monocytes and macrophages. Treatment of monocytes/macrophages with lupeol (72 µM) and casearin G (4 µM) reduced the production of NO. Neither lupeol (< 969 µM) nor casearin G (< 55 µM) had cytotoxic effects on PBMC. Casearin G showed both cytotoxic (IC50, LC50) and cytostatic (GI50) effects against tumor cells, PC-3 (IC50 = 12.5 µM; GI50 = 13.3 µM; LC50 = 51.9 µM) and MCF-7 (IC50 = 112.8 µM; GI50 = 11.8 µM; LC50 = 49.4 µM), as well as a hemolytic effect (≥ 182 µM). Conclusions: These observations indicate that lupeol and casearin G might be useful compounds in the preparation of anti-inflammatory drugs, whereas casearin G might be useful in the elaboration of antitumor drugs.info:eu-repo/semantics/openAccessSociedad Médica de SantiagoRevista médica de Chile v.141 n.9 20132013-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872013000900007es10.4067/S0034-98872013000900007
institution	Scielo Chile
collection	Scielo Chile
language	Spanish / Castilian
topic	Antineoplastic agents phytogenic Diterpenes clerodane Pentacyclic triterpenes
spellingShingle	Antineoplastic agents phytogenic Diterpenes clerodane Pentacyclic triterpenes Dupuy L,Omar A Bonilla V,José A Murillo,Renato Taylor,Peter Abad,María Jesús González,Lorena Juliao A,Johanna Efecto in vitro de los terpenos lupeol y casearina G sobre células sanguíneas y tumorales
description	Background: The rainforest is an important source of natural compounds with therapeutic properties. Although there are many anti-inflammatory and antineoplastic drugs available to the clinician, there is an ongoing need for new therapeutic drugs with fewer serious adverse effects. Aim: To evaluate the in vitro cytotoxic effects of lupeol and casearin G on tumor cells, on phagocytic activity and nitric oxide (NO) production by blood mononuclear cells. Material and Methods: The cytotoxic effect of these compounds on cell lines MCF-7 (human breast adenocarcinoma) and PC-3 (human prostate cancer) was measured by a colorimetric assay (MTS/PMS) and the sulphorhodamine B assay. Peripheral blood mononuclear cells were obtained from eight healthy volunteers. The effect of these compounds on nitric oxide (NO) production was measured using the Griess reaction. Their effect on phagocytic activity of PBMC was also evaluated. Results: Lupeol (≥ 2 mM) resulted in a reduction of both the phagocytic index and the percentage of phagocytic monocytes and macrophages. Treatment of monocytes/macrophages with lupeol (72 µM) and casearin G (4 µM) reduced the production of NO. Neither lupeol (< 969 µM) nor casearin G (< 55 µM) had cytotoxic effects on PBMC. Casearin G showed both cytotoxic (IC50, LC50) and cytostatic (GI50) effects against tumor cells, PC-3 (IC50 = 12.5 µM; GI50 = 13.3 µM; LC50 = 51.9 µM) and MCF-7 (IC50 = 112.8 µM; GI50 = 11.8 µM; LC50 = 49.4 µM), as well as a hemolytic effect (≥ 182 µM). Conclusions: These observations indicate that lupeol and casearin G might be useful compounds in the preparation of anti-inflammatory drugs, whereas casearin G might be useful in the elaboration of antitumor drugs.
author	Dupuy L,Omar A Bonilla V,José A Murillo,Renato Taylor,Peter Abad,María Jesús González,Lorena Juliao A,Johanna
author_facet	Dupuy L,Omar A Bonilla V,José A Murillo,Renato Taylor,Peter Abad,María Jesús González,Lorena Juliao A,Johanna
author_sort	Dupuy L,Omar A
title	Efecto in vitro de los terpenos lupeol y casearina G sobre células sanguíneas y tumorales
title_short	Efecto in vitro de los terpenos lupeol y casearina G sobre células sanguíneas y tumorales
title_full	Efecto in vitro de los terpenos lupeol y casearina G sobre células sanguíneas y tumorales
title_fullStr	Efecto in vitro de los terpenos lupeol y casearina G sobre células sanguíneas y tumorales
title_full_unstemmed	Efecto in vitro de los terpenos lupeol y casearina G sobre células sanguíneas y tumorales
title_sort	efecto in vitro de los terpenos lupeol y casearina g sobre células sanguíneas y tumorales
publisher	Sociedad Médica de Santiago
publishDate	2013
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872013000900007
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Efecto in vitro de los terpenos lupeol y casearina G sobre células sanguíneas y tumorales

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