Mutación del gen KRAS en el cáncer de colon y recto

Background: KRAS oncogene is involved in colorectal carcinogenesis in 22 to 45% of cases. Aim: To determine the frequency, types and distribution of KRAS mutations in colorectal cancer. Material and Methods: KRAS mutations studies were carried out in primary tumors and metastases of colo-rectal canc...

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Autores principales:	Roa,Iván, Sánchez,Tamara, Majlis,Alejandro, Schalper,Kurt
Lenguaje:	Spanish / Castilian
Publicado:	Sociedad Médica de Santiago 2013
Materias:	Colonic neoplasms Rectal neoplasms Sarcospan (Kras oncogene-associated gene) protein, human
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872013000900009
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spelling	oai:scielo:S0034-988720130009000092014-09-02Mutación del gen KRAS en el cáncer de colon y rectoRoa,IvánSánchez,TamaraMajlis,AlejandroSchalper,Kurt Colonic neoplasms Rectal neoplasms Sarcospan (Kras oncogene-associated gene) protein, human Background: KRAS oncogene is involved in colorectal carcinogenesis in 22 to 45% of cases. Aim: To determine the frequency, types and distribution of KRAS mutations in colorectal cancer. Material and Methods: KRAS mutations studies were carried out in primary tumors and metastases of colo-rectal cancer from 56 women aged 60 ± 14 years and 53 men aged 61 ± 11 years. Formalin fixed and paraffin embedded tissue samples were evaluated using RFLP (Restriction Fragment Length Polymorphism) and direct sequencing. Results: Primary tumors were located in the colon and rectum in 82 (75.2%) and 24 cases (20%), respectively. In three cases the extraction site of the tumor sample was unknown. In 46 cases (42.2%) KRAS mutations were demonstrated. The main point mutations were located in codon 12 (80.4%), G12D (39.1%), G12V (24.2%), G12S (6.5%), G12A (4.3%); G12C (4.3%), G12R (2.1%) and 19.6% at codon 13 (G13D). No differences were demonstrated in the frequency and distribution of mutations by gender, age, primary versus metastatic tumors or tumor location. Conclusions: In this series, 42% of colorectal cancer tissue samples had KRAS mutations. Their frequency and distribution are similar to those reported in the literature, except for G12C mutation.info:eu-repo/semantics/openAccessSociedad Médica de SantiagoRevista médica de Chile v.141 n.9 20132013-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872013000900009es10.4067/S0034-98872013000900009
institution	Scielo Chile
collection	Scielo Chile
language	Spanish / Castilian
topic	Colonic neoplasms Rectal neoplasms Sarcospan (Kras oncogene-associated gene) protein, human
spellingShingle	Colonic neoplasms Rectal neoplasms Sarcospan (Kras oncogene-associated gene) protein, human Roa,Iván Sánchez,Tamara Majlis,Alejandro Schalper,Kurt Mutación del gen KRAS en el cáncer de colon y recto
description	Background: KRAS oncogene is involved in colorectal carcinogenesis in 22 to 45% of cases. Aim: To determine the frequency, types and distribution of KRAS mutations in colorectal cancer. Material and Methods: KRAS mutations studies were carried out in primary tumors and metastases of colo-rectal cancer from 56 women aged 60 ± 14 years and 53 men aged 61 ± 11 years. Formalin fixed and paraffin embedded tissue samples were evaluated using RFLP (Restriction Fragment Length Polymorphism) and direct sequencing. Results: Primary tumors were located in the colon and rectum in 82 (75.2%) and 24 cases (20%), respectively. In three cases the extraction site of the tumor sample was unknown. In 46 cases (42.2%) KRAS mutations were demonstrated. The main point mutations were located in codon 12 (80.4%), G12D (39.1%), G12V (24.2%), G12S (6.5%), G12A (4.3%); G12C (4.3%), G12R (2.1%) and 19.6% at codon 13 (G13D). No differences were demonstrated in the frequency and distribution of mutations by gender, age, primary versus metastatic tumors or tumor location. Conclusions: In this series, 42% of colorectal cancer tissue samples had KRAS mutations. Their frequency and distribution are similar to those reported in the literature, except for G12C mutation.
author	Roa,Iván Sánchez,Tamara Majlis,Alejandro Schalper,Kurt
author_facet	Roa,Iván Sánchez,Tamara Majlis,Alejandro Schalper,Kurt
author_sort	Roa,Iván
title	Mutación del gen KRAS en el cáncer de colon y recto
title_short	Mutación del gen KRAS en el cáncer de colon y recto
title_full	Mutación del gen KRAS en el cáncer de colon y recto
title_fullStr	Mutación del gen KRAS en el cáncer de colon y recto
title_full_unstemmed	Mutación del gen KRAS en el cáncer de colon y recto
title_sort	mutación del gen kras en el cáncer de colon y recto
publisher	Sociedad Médica de Santiago
publishDate	2013
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872013000900009
work_keys_str_mv	AT roaivan mutaciondelgenkrasenelcancerdecolonyrecto AT sancheztamara mutaciondelgenkrasenelcancerdecolonyrecto AT majlisalejandro mutaciondelgenkrasenelcancerdecolonyrecto AT schalperkurt mutaciondelgenkrasenelcancerdecolonyrecto
_version_	1718436711811252224

Mutación del gen KRAS en el cáncer de colon y recto

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