Expresión y amplificación del gen HER2 en el cáncer gástrico avanzado

Background: Overexpression/amplification of the HER2 gene in advanced gastric cancer is a predictor of response to adjuvant therapy with monoclonal antibodies. Aim: To determine the frequency of HER2 gene overexpression and amplification in advanced gastric cancer. Material and Methods: One hundred...

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Autores principales:	Roa,Iván, Slater,Jeannie, Carvajal,Daniel, Schalper,Kurt, Toro,Gonzalo de, Ares,Raúl, Game,Anakaren, León,Jorge, Aretxabala,Xabier de
Lenguaje:	Spanish / Castilian
Publicado:	Sociedad Médica de Santiago 2013
Materias:	Gene amplification HER2, protein, human in situ hibridization, fluorescence Immunohistochemistry
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872013001100007
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spelling	oai:scielo:S0034-988720130011000072014-09-01Expresión y amplificación del gen HER2 en el cáncer gástrico avanzadoRoa,IvánSlater,JeannieCarvajal,DanielSchalper,KurtToro,Gonzalo deAres,RaúlGame,AnakarenLeón,JorgeAretxabala,Xabier de Gene amplification HER2, protein, human in situ hibridization, fluorescence Immunohistochemistry Background: Overexpression/amplification of the HER2 gene in advanced gastric cancer is a predictor of response to adjuvant therapy with monoclonal antibodies. Aim: To determine the frequency of HER2 gene overexpression and amplification in advanced gastric cancer. Material and Methods: One hundred nine advanced gastric cancer biopsy specimens, from 76 men and 33 women aged 67 ± 14 and 62 ± 12 years respectively, were selected. Three histological patterns (diffuse, intestinal and mixed) were recognized. Automated immunohistochemistry was performed with monoclonal c-erbB-2 (NCL-356) Novocastra. Fluorescent in situ hybridization (FISH) for HER2 was performed in positive cases. Results: In 39% of cases, immunohistochemical staining was negative. It was 1+, 2+ and 3+ positive in 15, 36 and 11% of cases, respectively. It was positive in 16% and 3% of intestinal type and mixed carcinomas, respectively. It was negative in all diffuse carcinomas. FISH was performed in 39 (2 +) cases and in 11 (3 +) cases. The gene amplification was positive in two (2 +) and 11 (3 +) cases (11.9%). The overall concordance between immunohistochemical staining and in situ hybridization was 85%. Conclusions: In advanced gastric cancer, HER2 gene overexpression or amplification was observed in 11% and 12% of cases, respectively.info:eu-repo/semantics/openAccessSociedad Médica de SantiagoRevista médica de Chile v.141 n.11 20132013-11-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872013001100007es10.4067/S0034-98872013001100007
institution	Scielo Chile
collection	Scielo Chile
language	Spanish / Castilian
topic	Gene amplification HER2, protein, human in situ hibridization, fluorescence Immunohistochemistry
spellingShingle	Gene amplification HER2, protein, human in situ hibridization, fluorescence Immunohistochemistry Roa,Iván Slater,Jeannie Carvajal,Daniel Schalper,Kurt Toro,Gonzalo de Ares,Raúl Game,Anakaren León,Jorge Aretxabala,Xabier de Expresión y amplificación del gen HER2 en el cáncer gástrico avanzado
description	Background: Overexpression/amplification of the HER2 gene in advanced gastric cancer is a predictor of response to adjuvant therapy with monoclonal antibodies. Aim: To determine the frequency of HER2 gene overexpression and amplification in advanced gastric cancer. Material and Methods: One hundred nine advanced gastric cancer biopsy specimens, from 76 men and 33 women aged 67 ± 14 and 62 ± 12 years respectively, were selected. Three histological patterns (diffuse, intestinal and mixed) were recognized. Automated immunohistochemistry was performed with monoclonal c-erbB-2 (NCL-356) Novocastra. Fluorescent in situ hybridization (FISH) for HER2 was performed in positive cases. Results: In 39% of cases, immunohistochemical staining was negative. It was 1+, 2+ and 3+ positive in 15, 36 and 11% of cases, respectively. It was positive in 16% and 3% of intestinal type and mixed carcinomas, respectively. It was negative in all diffuse carcinomas. FISH was performed in 39 (2 +) cases and in 11 (3 +) cases. The gene amplification was positive in two (2 +) and 11 (3 +) cases (11.9%). The overall concordance between immunohistochemical staining and in situ hybridization was 85%. Conclusions: In advanced gastric cancer, HER2 gene overexpression or amplification was observed in 11% and 12% of cases, respectively.
author	Roa,Iván Slater,Jeannie Carvajal,Daniel Schalper,Kurt Toro,Gonzalo de Ares,Raúl Game,Anakaren León,Jorge Aretxabala,Xabier de
author_facet	Roa,Iván Slater,Jeannie Carvajal,Daniel Schalper,Kurt Toro,Gonzalo de Ares,Raúl Game,Anakaren León,Jorge Aretxabala,Xabier de
author_sort	Roa,Iván
title	Expresión y amplificación del gen HER2 en el cáncer gástrico avanzado
title_short	Expresión y amplificación del gen HER2 en el cáncer gástrico avanzado
title_full	Expresión y amplificación del gen HER2 en el cáncer gástrico avanzado
title_fullStr	Expresión y amplificación del gen HER2 en el cáncer gástrico avanzado
title_full_unstemmed	Expresión y amplificación del gen HER2 en el cáncer gástrico avanzado
title_sort	expresión y amplificación del gen her2 en el cáncer gástrico avanzado
publisher	Sociedad Médica de Santiago
publishDate	2013
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872013001100007
work_keys_str_mv	AT roaivan expresionyamplificaciondelgenher2enelcancergastricoavanzado AT slaterjeannie expresionyamplificaciondelgenher2enelcancergastricoavanzado AT carvajaldaniel expresionyamplificaciondelgenher2enelcancergastricoavanzado AT schalperkurt expresionyamplificaciondelgenher2enelcancergastricoavanzado AT torogonzalode expresionyamplificaciondelgenher2enelcancergastricoavanzado AT aresraul expresionyamplificaciondelgenher2enelcancergastricoavanzado AT gameanakaren expresionyamplificaciondelgenher2enelcancergastricoavanzado AT leonjorge expresionyamplificaciondelgenher2enelcancergastricoavanzado AT aretxabalaxabierde expresionyamplificaciondelgenher2enelcancergastricoavanzado
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Expresión y amplificación del gen HER2 en el cáncer gástrico avanzado

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